Production of alkaline proteases by alkalophilic Bacillus subtilis during recycling animal and plant wastes

2016 ◽  
Vol 15 (47) ◽  
pp. 2698-2702 ◽  
Author(s):  
Hassan Al-Abdalall Amira ◽  
Marshid Al-Khaldi Eida





2010 ◽  
Vol 41 (2) ◽  
pp. 467-476 ◽  
Author(s):  
Ashwani Sanghi ◽  
Neelam Garg ◽  
V.K. Gupta ◽  
Ashwani Mittal ◽  
R.C. Kuhad


1988 ◽  
Vol 27 (4) ◽  
Author(s):  
Kenji Kimura ◽  
Akira Tsukamoto ◽  
Yasumasa Ishii ◽  
Toshiya Takano ◽  
Kunio Yamane


2021 ◽  
Author(s):  
Tayachew Desalegn ◽  
◽  
Ketema Bacha ◽  
Mesfin Tafesse ◽  
Chandran Masi ◽  
...  

Protease also called proteinase or peptidase is a digestive enzyme that is categorized under proteolytic enzymes and it has great potential in industrial application. Extracellular proteases are used in a variety of industries because they exhibit practically all of the characteristics needed for biotech applications such as detergent, bioremediation, food, and leather processing. In the synthesis of all three major types of acidic, neutral, and alkaline proteases, microbial sources have dominated an unbeatable area. Alkaline proteases are a large group of industrial enzymes formed by a wide variety of species, including animals, fungi, and bacteria. The fermentation method serves to make bacteria, fungi, and yeast alkaline proteases. Proteases are produced in large quantities by Gram-positive bacteria, especially those belonging to the Bacillus genus. Following standard procedures, the bacterial isolates PMOJ-01 and PMOJ-05 with the prominent zone of clearance and efficient enzyme development were further characterized to the genus level. Moreover, the growth conditions for the highest protease production were optimized with different pH, temperatures, and NaCl concentrations, in the results of PMOJ-01 and PMOJ- 05 pH (7 and 8), temperatures 45oC, and 1% NaCl concentrations both cases respectively. The proteases activities from PMOJ-01, Pseudomonas aeruginosa, and PMOJ-05, Bacillus subtilis were most active at pH 7.0 and pH 8.0 and temperature at 35oC and 45oC, respectively. The enzyme activity and the total solid protease sample of the crude enzyme of Pseudomonas aeruginosa and Bacillus subtilis were 0.299 U/ mL and 0.289 U/ mL, 1.37±0.14 U/mg, and 1.199 U/mg respectively. The effect on dehairing, distaining, and scum removal revealed that the purified protease enzyme of PMOJ-01 and PMOJ-05 can be used in detergent and leather industries.



1997 ◽  
Vol 83 (6) ◽  
pp. 758-763 ◽  
Author(s):  
C.M. Yeh ◽  
H.K. Chang ◽  
H.M. Hsieh ◽  
K. Yoda ◽  
M. Yamasaki ◽  
...  


1985 ◽  
Vol 161 (1) ◽  
pp. 158-163 ◽  
Author(s):  
T Kudo ◽  
J Yoshitake ◽  
C Kato ◽  
R Usami ◽  
K Horikoshi




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