Molecular identification of original plants of Spica prunellae based on ITS sequence

Ficus spp. belongs to the tribe Ficeae in the Moraceae family. Many members of this genus have been collected and grown in Bogor Botanic Gardens. There are 519 living collections of Ficus conserved since 1817, and 13 of them have not been identified until the species level. This research aimed to identify the Ficus sp. originated from Kaur Selatan (Bengkulu) using morphological and molecular approaches. Morphological characterization and herbarium specimen observation have been carried out to identify the Ficus sp. The molecular approach was conducted through DNA barcoding using ITS primer. The molecular identification using ITS sequence showed that Ficus sp. is Ficus crassiramea with 99.87% similarity to the sequence in NCBI. Morphological observation through herbarium specimen showed that there are 9 vegetative characters specific to Ficus crassiramea.

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WildTermitomycesSpecies Collected from Ondo and Ekiti States Are More Related to African Species as Revealed by ITS Region of rDNA

The Scientific World JOURNAL ◽

10.1100/2012/689296 ◽

2012 ◽

Vol 2012 ◽

pp. 1-5 ◽

Cited By ~ 2

Author(s):

Victor Olusegun Oyetayo

Keyword(s):

Sequence Analysis ◽

Phylogenetic Tree ◽

Molecular Identification ◽

Internal Transcribed Spacer ◽

Its Region ◽

Its Sequence ◽

African Countries ◽

African Species ◽

Its Sequence Analysis ◽

Degree Of Similarity

Molecular identification of eighteenTermitomycesspecies collected from two states, Ondo and Ekiti in Nigeria was carried out using the internal transcribed spacer (ITS) region. The amplicons obtained from rDNA ofTermitomycesspecies were compared with existing sequences in the NCBI GenBank. The results of the ITS sequence analysis discriminated between all theTermitomycesspecies (obtained from Ondo and Ekiti States) andTermitomycessp. sequences obtained from NCBI GenBank. The degree of similarity of T1 to T18 to gene ofTermitomycessp. obtained from NCBI ranges between 82 and 99 percent.Termitomycesspecies from Garbon with ascension number AF321374 was the closest relative of T1 to T18 except T12 that has T. eurhizus and T. striatus as the closet relative. Phylogenetic tree generated with ITS sequences obtained from NCBI GenBank data revealed that T1 to T18 are more related toTermitomycesspecies indigenous to African countries such as Senegal, Congo, and Gabon.

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Molecular Identification of Zoysia japonica and Zoysia sinica (Zoysia Species) Based on ITS Sequence Analyses and CAPS

Korean Journal of Horticultural Science&Technology ◽

10.12972/kjhst.20170037 ◽

2017 ◽

Vol 35 (3) ◽

Keyword(s):

Molecular Identification ◽

Its Sequence ◽

Zoysia Japonica ◽

Sequence Analyses

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Confirmation of Tuntun Angin (Elaeocarpus floribundus) Taxonomic Status Using matK and ITS Sequences

Biosaintifika Journal of Biology & Biology Education ◽

10.15294/biosaintifika.v8i3.7406 ◽

2016 ◽

Vol 8 (3) ◽

pp. 393

Author(s):

Dewi Indriyani Roslim ◽

Siti Khumairoh ◽

Herman Herman

Keyword(s):

Molecular Identification ◽

Intergenic Spacer ◽

Biology Education ◽

Taxonomic Status ◽

Its Sequences ◽

Morphological Identification ◽

Its Sequence ◽

Query Cover ◽

Identification Techniques ◽

Scientific Name

Tuntun angin is one of important floodplain plants in and around Kajuik Lake located in Riau Province, Indonesia. Morphological identification shows that the scientific name of this plant is Elaeocarpus floribundus. The study aimed to confirm the taxonomic status of tuntun angin using matK and nuclear intergenic spacer (ITS) sequences. The methods included fresh leaf DNA isolation, polymerase chain reaction, electrophoresis, sequencing, and data analysis using BLASTn program and MEGA software version 6.06 programs. The results showed that the matK sequence (519 bp) of tuntun angin had highest similarity to E. floribundus matK sequence that was available in GenBank. It was supported by the high max score (937), low E-value (0.0), high identity value (100%), and high query cover (100%). However, the ITS sequence of tuntun angin did not show similarity to E. floribundus ITS sequence because there was no database of the sequence in GenBank. This study was able to confirm the taxonomic status of tuntun angin as E. floribundus using matK sequence and also showed that morphological and molecular identification techniques were complementary to each other. Moreover, this study enriched the DNA sequence database of E. floribundus in GenBank which will be useful for this species’ molecular identification.How to CiteRoslim, D. I., Khumairoh, S. & Herman, H. (2016). Confirmation of Tuntun Angin (Elaeocarpus floribundus) Taxonomic Status Using matK and ITS Sequences. Biosaintifika: Journal of Biology & Biology Education, 8(3), 393-400.

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Molecular identification ofVerbascum thapsusl. (Ban Tambaaku) and its ITS sequence comparison with otherVerbascuml. species

Medicinal Plants - International Journal of Phytomedicines and Related Industries ◽

10.5958/j.0975-6892.6.1.003 ◽

2014 ◽

Vol 6 (1) ◽

pp. 21 ◽

Cited By ~ 2

Author(s):

Devendra Kumar Srivastava ◽

Manjit Inder Singh Saggoo

Keyword(s):

Molecular Identification ◽

Sequence Comparison ◽

Its Sequence

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ITS sequence analysis used for molecular identification of the Bupleurum species from northwestern China

Phytomedicine ◽

10.1016/j.phymed.2007.04.009 ◽

2007 ◽

Vol 14 (6) ◽

pp. 416-423 ◽

Cited By ~ 24

Author(s):

Zhi-Ye Yang ◽

Zhi Chao ◽

Ke-Ke Huo ◽

Hui Xie ◽

Zhi-Peng Tian ◽

...

Keyword(s):

Sequence Analysis ◽

Molecular Identification ◽

Northwestern China ◽

Its Sequence ◽

Its Sequence Analysis

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Electron microscopy in diagnostic virology

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100110702 ◽

1984 ◽

Vol 42 ◽

pp. 70-73

Author(s):

S. E. Miller

Keyword(s):

Electron Microscopy ◽

Tissue Culture ◽

Molecular Identification ◽

Point Of View ◽

Negative Stain ◽

Viral Diagnosis ◽

Electron Microscopist ◽

Diagnostic Virology

The techniques for detecting viruses are many and varied including FAT, ELISA, SPIRA, RPHA, SRH, TIA, ID, IEOP, GC (1); CF, CIE (2); Tzanck (3); EM, IEM (4); and molecular identification (5). This paper will deal with viral diagnosis by electron microscopy and will be organized from the point of view of the electron microscopist who is asked to look for an unknown agent--a consideration of the specimen and possible agents rather than from a virologist's view of comparing all the different viruses. The first step is to ascertain the specimen source and select the method of preparation, e. g. negative stain or embedment, and whether the sample should be precleared by centrifugation, concentrated, or inoculated into tissue culture. Also, knowing the type of specimen and patient symptoms will lend suggestions of possible agents and eliminate some viruses, e. g. Rotavirus will not be seen in brain, nor Rabies in stool, but preconceived notions should not prejudice the observer into missing an unlikely pathogen.

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