scholarly journals Establishment of hairy root culture and production of secondary metabolites in Coleus (Coleus forskohlii)

2014 ◽  
Vol 8 (1) ◽  
pp. 58-62 ◽  
Author(s):  
Pandey Rajiv ◽  
Krishnasamy V. ◽  
Kumaravadivel N. ◽  
Rajamani K.
Keyword(s):  
Secondary Metabolites ◽  
Hairy Root ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Coleus Forskohlii

Hairy Root Culture for In Vitro Production of Secondary Metabolites: A Promising Biotechnological Approach

2018 ◽  
pp. 235-250
Author(s):  
Ravi Shankar Singh ◽  
Tirthartha Chattopadhyay ◽  
Dharamsheela Thakur ◽  
Nitish Kumar ◽  
Tribhuwan Kumar ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Hairy Root ◽  
Root Culture ◽  
Hairy Root Culture ◽  
In Vitro Production ◽  
Biotechnological Approach ◽  
Vitro Production

Hairy Roots

2021 ◽  
pp. 219-252
Keyword(s):  
Secondary Metabolites ◽  
Hairy Root ◽  
Hairy Roots ◽  
High Growth ◽  
Root Culture ◽  
Hairy Root Culture ◽  
High Growth Rate ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Transformed Root Cultures

Agrobacterium rhizogenes induces hairy root disease in plants. The neoplastic (cancerous) roots produced by A. rhizogenes infection, when cultured in hormone free medium, show high growth rate and genetic stability. These genetically transformed root cultures can produce levels of secondary metabolites comparable to that of intact plants. Several elicitation methods can be used to further enhance the production and accumulation of secondary metabolites. Thus, hairy root culture offer promise for high production and productivity of valuable secondary metabolites in many plants. Hairy roots can also produce recombinant proteins from transgenic roots, and thereby hold immense potential for pharmaceutical industry. Hairy root cultures can be used to elucidate the intermediates and key enzymes involved in the biosynthesis of secondary metabolites, and for phytoremediation due to their abundant neoplastic root proliferation property. Various applications of hairy root cultures and potential problems associated with them are discussed in this chapter.

Hairy Root Culture an Alternative for Bioactive Compound Production from Medicinal Plants

2020 ◽  
Vol 22 (1) ◽  
pp. 136-149
Author(s):  
Arpita Roy
Keyword(s):  
Medicinal Plants ◽  
Secondary Metabolites ◽  
Bioactive Compounds ◽  
Hairy Root ◽  
Scale Up ◽  
Root Culture ◽  
Bioactive Compound ◽  
Hairy Root Culture ◽  
Alternative Methods ◽  
Wide Range

: Medicinal plants produce a diverse group of phytocompounds like anthraquinones, alkaloids, anthocyanins, flavonoids, saponins, and terpenes which are used in pharmaceutical, perfume, cosmetics, dye and flavor industries. Commercial source of these metabolites is field-grown plants, which are generally influenced by seasonal changes. Biotechnology possesses a significant role in production of high-value secondary metabolites. By incorporating biotechnological methods, it is feasible to manage biosynthetic pathways of the plant to enhance phytocompound production that is of pharmaceutical interest. Plant cell suspension, shoot, adventitious root and hairy root culture are considered as alternative methods for important bioactive compound production. These methods are controllable, sustainable and overcome several inconveniences for large scale secondary metabolites production. At present research on hairy root culture for valuable bioactive compound production has gained a lot of attention. Agrobacterium rhizogenes is an agent which causes hairy root disease in a plant and this leads to the neoplastic growth of root which is characterized by higher growth rate and genetic stability. Various studies explore the hairy root culture for production of a wide range of bioactive compounds. Scale-up of hairy root culture using bioreactors has provided an opportunity to enhance bioactive compound production at the commercial level. The present review discusses the role of hairy root culture in the production of valuable bioactive compounds, the effect of culture parameters on bioactive compound production and bioreactor applications.

Hairy Roots

2022 ◽  
pp. 735-759
Author(s):  
Pradip Chandra Deka
Keyword(s):  
Secondary Metabolites ◽  
Hairy Root ◽  
Hairy Roots ◽  
High Growth ◽  
Root Culture ◽  
Hairy Root Culture ◽  
High Growth Rate ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Transformed Root Cultures

Agrobacterium rhizogenes induces hairy root disease in plants. The neoplastic (cancerous) roots produced by A. rhizogenes infection, when cultured in hormone free medium, show high growth rate and genetic stability. These genetically transformed root cultures can produce levels of secondary metabolites comparable to that of intact plants. Several elicitation methods can be used to further enhance the production and accumulation of secondary metabolites. Thus, hairy root culture offer promise for high production and productivity of valuable secondary metabolites in many plants. Hairy roots can also produce recombinant proteins from transgenic roots, and thereby hold immense potential for pharmaceutical industry. Hairy root cultures can be used to elucidate the intermediates and key enzymes involved in the biosynthesis of secondary metabolites, and for phytoremediation due to their abundant neoplastic root proliferation property. Various applications of hairy root cultures and potential problems associated with them are discussed in this chapter.

scholarly journals HAIRY ROOT CULTURE OF AN IMPORTANT MEDICINAL PLANT: COLEUS FORSKOHLII

2011 ◽  
Vol 3 (2) ◽  
pp. 82-89 ◽  
Author(s):  
UMA MAHESWARI R ◽  
◽  
SELVAMURUGAN C ◽  
JAYABARATH J ◽  
LAKSHMI PRABHA A
Keyword(s):  
Medicinal Plant ◽  
Hairy Root ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Coleus Forskohlii ◽  
Important Medicinal Plant

scholarly journals ENHANCED ANTHOCYANIN PRODUCTION IN HAIRY ROOT CULTURE OF DAUCUS CAROTA BY FUNGAL ELICITOR

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 694b-694 ◽  
Author(s):  
Chano H. Kim ◽  
Jonq H. Park ◽  
In S. Chung ◽  
Sung R. Kim ◽  
Seung W. Lee
Keyword(s):  
Secondary Metabolites ◽  
Fluidized Bed ◽  
Hairy Root ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Fungal Elicitor ◽  
High Density Culture ◽  
Fluidized Bed Bioreactor ◽  
Fungal Elicitors ◽  
Anthocyanin Production

Secondary metabolite production by plant cell culture has been become of interest because of its commercial value in use. However, cultured plant cells usually yield lower levels of secondary metabolites than those of intact plants. In order to improve the anthocyanin productivity in hairy root culture of Daucus carota, fungal elicitors from 8 species of Fungi were examined. Through the studies of fungal elicitors in this work, it was turned out that fungal elicitors were very effective to improve the yield of anthocyanin. Despite of its low yield of anthocyanin, high density culture of hairy roots is achieved in fluidized-bed bioreactor, Anthocyanin production in fluidized-bed bioreactor with fungal elicitor treatment was increased greatly. We are currently researching more detailed aeration effects and scale-up in air-lift bioreactors. And these studies could provide important data to establish mass production system for secondary metabolites.

Application of Hairy Root Culture for Bioactive Compounds Production in Medicinal Plants

2020 ◽  
Vol 21 ◽  
Author(s):  
Caili Li ◽  
Meizhen Wang
Keyword(s):  
Medicinal Plants ◽  
Secondary Metabolites ◽  
Medicinal Plant ◽  
Bioactive Compounds ◽  
Hairy Root ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Ecological Problems ◽  
Herbal Plants ◽  
Natural Drugs

: Medicinal plants are rich sources of natural bioactive compounds used to treat many diseases. With the development of the health industry, the market demands for Chinese medicine have increased rapidly in recent years. However, over-utilization of herbal plants would cause serious ecological problems. Therefore, an effective approach should be developed to produce the pharmaceutically important natural drugs. Hairy root culture induced by Agrobacterium rhizogenes has been considered to be an effective tool to produce secondary metabolites that are normally biosynthesized in the roots or even in the aerial organs of mature plants. This review aims to summarize current progress on medicinal plant hairy root culture for bioactive compounds production. It presents the stimulating effects of various biotic and abiotic elicitors on the accumulation of secondary metabolites. Synergetic effects on the production of natural products by a combination of different elicitors or other strategies are also included. The transgenic system has promising prospects to increase bioactive compounds content by introducing their biosynthetic or regulatory genes into medicinal plant hairy root. It offers great potential to further increase secondary metabolites yield by the integration of manipulating pathway genes with elicitors and other strategies. Besides, successful examples of bioactive compounds production by hairy root culture in bioreactor are introduced. At last, advances on two valuable pharmaceuticals production in the hairy root cultures are illustrated in detail.

A Simple and Efficient Protocol for Hairy Root Culture of Arabidopsis Thaliana

2021 ◽  
Author(s):  
Pijush Paul ◽  
Sukanya Majumdar ◽  
Sumita Jha
Keyword(s):  
Arabidopsis Thaliana ◽  
Secondary Metabolites ◽  
Hairy Root ◽  
Plant Secondary Metabolites ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Bacterial Suspension ◽  
Root Induction ◽  
Role Players ◽  
High Transformation

Abstract Hairy root culture (HRC) is a valuable biotechnological tool for the production of plant secondary metabolites. Secondary metabolome study of Arabidopsis thaliana can be helpful in understanding the biological roles of various secondary metabolites present in it and HRC constructs the base of such study. The present work deals with the establishment of Agrobacterium rhizogenes strains transformed HRC of A. thaliana with very high transformation frequency resulting in long term hairy root cultures grown in hormone free media. Optimization of culture medium and standardisation of co-cultivation period are the key role players in obtaining high frequency of hairy roots. Four days of preculture in CIM medium and five min of co-cultivation in the bacterial suspension were found to be optimal conditions for root induction. This protocol could become a powerful tool for transcriptomics and proteomics-based studies for different transgenic root lines of A. thaliana.

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