Molecular identification of leaf litter fungi potential for cellulose degradation

The species diversity and distribution of 3 ecological groups of fungi (viz., foliar endophytes, phellophytes, and leaf litter fungi of 6 tree hosts of a dry thorn forest and 6 tree hosts of an evergreen forest of southern India) were studied. As the methods of sampling and the isolation procedure were maintained constant, the results could be compared across hosts and fungal assemblages. The diversity of endophytes was less in both forests, owing to host recurrence. The litter fungi showed a higher diversity in both forests when compared with endophytes or phellophytes. There was little overlap between the species assemblages of the 3 ecological groups of fungi. Hence, to be cost effective, diversity estimation exercises for fungi in the tropics should include different fungal substrates rather than one substrate (or ecological group); it appears that even small sample sizes of different substrates would provide an approximation that is the near-real species diversity, rather than using large sample sizes of any one particular indicator group of fungi.

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LEAF LITTER FUNGI ISOLATED IN BACH MA NATIONAL PARK, VIET NAM

Vietnam Journal of Science and Technology ◽

10.15625/2525-2518/55/1a/12380 ◽

2018 ◽

Vol 55 (1A) ◽

pp. 37 ◽

Cited By ~ 1

Author(s):

Le Thi Hoang Yen

Keyword(s):

Leaf Litter ◽

National Park ◽

Morphological Study ◽

Viet Nam ◽

Single Spore ◽

Moist Chamber ◽

Surface Disinfection ◽

Isolation Methods ◽

Litter Fungi

A survey of fungi from leaf litter at Bach Ma National Park in Viet Nam was initiated. Using surface disinfection and moist chamber with single spore isolation methods, 102 isolates were obtained and of those, 70 isolates were selected for further morphological study. They belong to 5 classes, 9 orders, 14 families, 33 genera, and 51 species. Among them, Ceratosporella deviata, Lateriramulosa sp., Isthmolongispora ampuliformis, Polylobatispora quinquecornuta, Scolecobasidium tricladiatum, Triglyphiuma labamense, Tricladiella pulvialis, Tripospermum myrti, Triscelophorus sp., Varicosporium elodeaea are newly recorded in Viet Nam.

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CELLULOLYTIC BACTERIA MANGROVE LEAF LITTER IN BANGKA ISLAND

Samakia : Jurnal Ilmu Perikanan ◽

10.35316/jsapi.v9i1.218 ◽

2018 ◽

Vol 9 (1) ◽

pp. 06-11

Author(s):

Ardiansyah Kurniawan ◽

Asep Awaludin Prihanto ◽

Suci Puspitasari ◽

Andi Kurniawan ◽

Euis Asriani ◽

...

Keyword(s):

Bacillus Subtilis ◽

Leaf Litter ◽

Agar Medium ◽

Cellulose Degradation ◽

Bacterial Isolates ◽

Cellulolytic Bacteria ◽

Clear Zone ◽

Biochemical Tests ◽

Positive Isolate ◽

Degradation Ability

The study aimed to obtain isolate of cellulolytic bacteria from leaf litter on mangrove in Bangka Island. Sampling was conducted on mangroves in Sungailiat, Bangka and Tukak Sadai, South Bangka district. The isolation was carried out using 1% enriched agarmedia of Carboxymetyl Cellulose (CMC). The bacterial isolates were tested with cellulolytic growth on 1% enriched CMC agar medium and lugol added at 72 hours. The clear zone resultingindicates cellulose degradation ability. The positive isolate of cellulolyticwas identified by biochemical tests. 1 of 5 isolates in Sungailiat positive cellulolytic mangroves and identified as Bacillus subtilis. 2 of 5 isolates in mangrove Tukak Sadai is positive as a cellulolytic bacteria and identified as Staphylococcus saproviticus and Bacillus cereus.

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