Multivariate Calibration Technique for the Spectrophotometric Quantification of Rasagiline in Bulk drug and Pharmaceutical Formulations

2020 ◽  
Vol 13 (2) ◽  
pp. 843
Author(s):  
D. Naveena Rani ◽  
K. S. Kokilambigai ◽  
K. S. Lakshmi
Keyword(s):  
Multivariate Calibration ◽  
Pharmaceutical Formulations ◽  
Calibration Technique ◽  
Bulk Drug

scholarly journals SPECTROPHOTOMETRIC QUANTIFICATION OF GRANISETRON IN BULK DRUG AND PHARMACEUTICAL FORMULATIONS EMPLOYING MULTIVARIATE CALIBRATION TECHNIQUE

2021 ◽  
Vol 10 (4) ◽  
pp. 3435-3439
Author(s):  
Aravinth R Mani
Keyword(s):  
Multivariate Calibration ◽  
Regression Coefficient ◽  
Ultra Violet ◽  
Pharmaceutical Formulations ◽  
Regression Method ◽  
Regression Equations ◽  
Calibration Technique ◽  
Multilinear Regression ◽  
Linear Plot ◽  
Bulk Drug

The study goal is to establish a sensitive, accurate, simple and validated Ultra violet (UV) spectrophotometric technique by multilinear regression method for the assessment of Granisetron in the bulk drug and pharmaceutical formulations. The multivariate calibration technique relies on linear regression equations and employs correlation between absorbance and concentration at five different wavelengths. The λmax of Granisetron was at 302 nm. The results were treated statistically. A linear plot was achieved in the concentration range of 10 - 30 μmol/L and regression coefficient was obtained to be 0.999. The percentage recovery was obtained in the range of 99.48 - 101.81 % w/w.

Stability-Indicating Methods for the Determination of Candesartan Cilexetil in Bulk Drug and Pharmaceutical Formulations

2013 ◽  
Vol 96 (3) ◽  
pp. 580-586 ◽  
Author(s):  
Yousry M Issa ◽  
Emad M Hussien ◽  
Magda M Ibrahim ◽  
Fatma M Abdel-Gawad ◽  
Saadia Barakat
Keyword(s):  
Mobile Phase ◽  
Candesartan Cilexetil ◽  
Degradation Products ◽  
Pharmaceutical Formulations ◽  
Stability Indicating ◽  
Bulk Powder ◽  
System Suitability ◽  
The Mean ◽  
Bulk Drug

Abstract Two stability-indicating methods were developed for the determination of candesartan cilexetil in the presence of its degradation products. The first method uses isocratic RP-HPLC with an Agilent C18 column. The mobile phase was phosphate buffer (pH = 2.8 ± 0.1)–acetonitrile (60 + 40, v/v). The flow rate was 2.0 mL/min, and the UV detection was at 254 nm. The second method depends on TLC-densitometric measurements of drug spots at 254 nm. The separation was carried out on silica gel 60 F254 plates using ethyl acetate–methanol–toluene– ammonia 33% (40 + 25 + 20 + 2, v/v/v/v) mobile phase. The methods were validated according to U.S. Pharmacopeia guidelines, and the acceptance criteria for accuracy, precision, linearity, specificity, robustness, LOD, LOQ, and system suitability were met in all cases. Linear ranges of the methods were 10.0–200.0 μg/mL and 1.0–9.0 μg/spot for HPLC and TLC, respectively. The proposed methods were successfully applied to the drug in bulk powder, in laboratory-prepared mixtures with its degradation products, and in commercially available tablets. The results were compared statistically at the 95% confidence level with each other. There were no significant differences between the mean recovery and precision of the two methods.

scholarly journals Stability Indicating HPLC Determination of Risperidone in Bulk Drug and Pharmaceutical Formulations

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Zarna R. Dedania ◽  
Ronak R. Dedania ◽  
Navin R. Sheth ◽  
Jigar B. Patel ◽  
Bhavna Patel
Keyword(s):  
Limit Of Detection ◽  
Degradation Products ◽  
Pharmaceutical Formulations ◽  
Assay Method ◽  
Pharmaceutical Dosage Forms ◽  
Stability Indicating ◽  
Stress Studies ◽  
Liquid Chromatographic Separation ◽  
Bulk Drug ◽  
Liquid Chromatographic

The objective of the current study was to develop a validated stability-indicating assay method (SIAM) for risperidone after subjecting it to forced decomposition under hydrolysis, oxidation, photolysis, and thermal stress conditions. The liquid chromatographic separation was achieved isocratically on a symmetry C18 column (5 μm size, 250 mm × 4.6 mm i.d.) using a mobile phase containing methanol: acetonitrile (80 : 20, v/v) at a flow rate of 1 mL/min and UV detection at 280 nm. Retention time of risperidone was found to be . The method was linear over the concentration range of 10–60 μg/mL with a limit of detection and quantitation of 1.79 and 5.44 μg/mL, respectively. The method has the requisite accuracy, specificity, sensitivity, and precision to assay risperidone in bulk form and pharmaceutical dosage forms. Degradation products resulting from the stress studies did not interfere with the detection of Risperidone, and the assay is thus stability indicating.

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