RP-HPLC and HPTLC methods for the estimation of carvedilol in bulk drug and pharmaceutical formulations

2006 ◽  
Vol 68 (6) ◽  
pp. 790 ◽  
Author(s):  
LJ Patel ◽  
BN Suhagia ◽  
PB Shah ◽  
RR Shah
Keyword(s):  
Pharmaceutical Formulations ◽  
Rp Hplc ◽  
Bulk Drug

scholarly journals Development of Analytical Profile of Lamotrigine and its API Formulation

2021 ◽  
pp. 111-114
Author(s):  
VISHAL CHAUDHARY ◽  
VASUNDHARA SAXENA
Keyword(s):  
Spectrophotometric Method ◽  
Quantitative Estimation ◽  
Pharmaceutical Formulations ◽  
Hplc Method ◽  
Active Pharmaceutical Ingredients ◽  
Pharmaceutical Ingredients ◽  
Rp Hplc ◽  
System Suitability ◽  
Bulk Drug

Objective: The objective of this review is to put a light on the development of lamotrigine and its active pharmaceutical ingredients formulation with proper demonstration. Method: In the present work, one of the most imperative spectrophotometric method which is RP-HPLC method has been developed for the quantitative estimation of lamotrigine in bulk and pharmaceutical formulations. UV spectrophotometric method which involves the determination of Lamotrigine in bulk and in bulk drug and pharmaceutical formulation has maximum absorption at 307.5nm in methanol. It obeys Beer’s and Lambert’s law in the concentration range of 5-45 µg/ml. A rapid and sensitive RP- HPLC Method with UV detection (270 nm) for routine analysis of Lamotrigine formulation was developed. Chromatography was performed with mobile phase containing a mixture of methanol and Phosphate buffer (65:35v/v) with flow rate 1.0 ml/min. In the range of 20-100 µg/ml, the linearity of lamotrigine shows a correlation co-efficient of 0.9998. The proposed method was validated by determining sensitivity and system suitability parameters.

scholarly journals RP-HPLC method development and validation for the estimation of antifungal drug terbinafine HCL in bulk and pharmaceutical dosage form

2018 ◽  
Vol 1 (1) ◽  
pp. 8-12
Author(s):  
R Sireesha ◽  
P Syam Vijayakar ◽  
V Pavan Kumar ◽  
B Sivagami ◽  
Pranabesh Sikdar ◽  
...  
Keyword(s):  
Method Development ◽  
Quantitative Estimation ◽  
Potassium Dihydrogen Phosphate ◽  
Pharmaceutical Formulations ◽  
Hplc Method ◽  
Dihydrogen Phosphate ◽  
Pharmaceutical Dosage Form ◽  
Terbinafine Hydrochloride ◽  
Rp Hplc ◽  
Bulk Drug

In the present work RP-HPLC method has been developed for the quantitative estimation of Terbinafine hydrochloride in bulk drug and pharmaceutical formulations. A rapid and sensitive RP-HPLC Method with PDA detection (220 nm) for routine analysis of in Bulk drug and Pharmaceutical formulation was developed. Chromatography was performed with mobile phase containing a mixture of Potassium dihydrogen phosphate and Acetonitrile (65:35 v/v) with flow rate 1.5 ml/min. The linearity was found to be in the range of 50-150 µg/ml with (r2=0.999). The proposed method was validated by determining sensitivity, accuracy, precision, LOD, LOQ and system suitability parameters according to ICH guidrelines.

scholarly journals Development and Validation of RP-HPLC method for Simultaneous Estimation of Metformin and Pioglitazone

2017 ◽  
Vol 5 (01) ◽  
pp. 48-50
Author(s):  
Uttam Kumar Agrawal ◽  
Jeyabalan Govindasamy
Keyword(s):  
Mobile Phase ◽  
Dosage Form ◽  
Pharmaceutical Formulations ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Drug Form ◽  
Rp Hplc ◽  
Ich Guidelines ◽  
Bulk Drug ◽  
Development And Validation

A new simple, accurate ,precise and reproducible RP-HPLC method has been developed for simultaneous estimation of metformin and pioglitazone in bulk drug form using proper HPLC system. The mobile phase consists of phosphate buffer and acetonitrile in the ratio of 35:65 at pH 3.4.The detection wavelength was carried out at 228 nm. The method was linear over the concentration range for metformin 50-100 μg/mL and for pioglitazone 20-180 μg/mL. The recoveries of metformin and pioglitazone were found to be 100.5 and 98.7% respectively. The validation of method was carried out utilizing ICH-guidelines. The described HPLC method method was successfully for the analysis of pharmaceutical formulations containing dosage form.

Analytical Method Development and Validation for Simultaneous estimation of Methotrexate and Hydroxychloroquine sulfate in bulk drug by using RP-HPLC

2021 ◽  
pp. 73-78
Author(s):  
Anupam Sharma ◽  
Amar Deep Ankalgi ◽  
Arti Devi ◽  
Vinay Pandit ◽  
Mahendra Singh Ashawat
Keyword(s):  
Method Development ◽  
Pharmaceutical Formulations ◽  
Simultaneous Estimation ◽  
Rp Hplc ◽  
Ich Guidelines ◽  
Validation Parameters ◽  
Chromatographic Procedure ◽  
Method Development And Validation ◽  
Bulk Drug ◽  
Development And Validation

A rapid isocratic chromatographic procedure for the analysis of methotrexate, hydroxychloroquine sulfate in bulk drug and pharmaceutical formulations was developed validated in the present study. The mobile phase consists of a mixture of Water: Acetonitrile: Tetrahydrofuran in the proportion of 50:40:10 and pH maintained to 3with perchloric acid. Retention time was found to be 3.0 and 3.7minutes for methotrexate and hydroxychloroquine sulfate respectively. The method was performing by using the C18 column, ODS Hypersil column with UV detection at 318nm, and flow rate of 1.0ml/min. The percentage of recovery for both drugs was found to be 99.99%. All validation parameters were within limits as per the ICH guidelines.

SIMULTANEOUS ESTIMATION OF CEFIXIME AND CLOXACILLIN IN BULK AND TABLET FORMULATION BY RP-HPLC METHOD

INDIAN DRUGS ◽  
2012 ◽  
Vol 49 (04) ◽  
pp. 23-29
Author(s):  
◽  
S. M Malipatil ◽  
L. V. Sonawane ◽  
R. C Hariprasanna
Keyword(s):  
High Performance ◽  
Pharmaceutical Formulations ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Good Resolution ◽  
Rp Hplc ◽  
Bulk Drug ◽  
Liquid Chromatographic ◽  
Simple Economic

A simple, economic, selective, precise and accurate high-performance liquid chromatographic (RP-HPLC) method for the analysis of cefixime and cloxacillin in both bulk drug and pharmaceutical formulations was developed and validated in the present study. This method has shown good resolution for cefixime, cloxacillin and formulation excipients of tablets. The mobile phase consisted of phosphate buffer (25 mM):methanol: acetonitrile (40: 45: 15 v/v/v), pH 4.6 adjusted with orthophosphoric acid. HPLC analysis of cefixime and cloxacillin was carried out at a wavelength of 225 nm with flow rate of 1.0 mL min-1. The method was quantitatively evaluated in terms of linearity, precision, accuracy (recovery), selectivity and robustness. The method is simple, convenient and suitable for the analysis of cefixime and cloxacillin in bulk and its formulation.

scholarly journals Validated RP-HPLC Method for Simultaneous Estimation of Domperidone and Naproxen in Bulk Drug and Formulations

2020 ◽  
pp. 701-706
Keyword(s):  
Flow Rate ◽  
Drug Concentration ◽  
Pharmaceutical Formulations ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Rp Hplc ◽  
Ich Guidelines ◽  
Bulk Drug ◽  
Isocratic Mode ◽  
Good Percentage

A new , precise , accurate and reproducible RP – HPLC method for simultaneous estimation of bulk and pharmaceutical formulations . Separation of Naproxen and Domperidone was successfully achieved. THERMO HYPERSIL , C 18 , 250mm X 4.6mm , 5μm , or equivalent in an isocratic mode utilizing Acetonitrile : Methanol : Water (20: 60:20 ) at a flow rate of 1.0 mL/ min and elute was monitored at 284nm , with a retention time of 2.341 and 5.225 minutes for Naproxen and Domperidone respectively. The method was validated and the response was found to be linear in the drug concentration range of 1μg /mL to 6μg/mL. The values of correlation coefficient was found to be 0.988 for Naproxen and 0.998 for Domperidone respectively. The LOD and LOQ for Naproxen was found to be 0.56, 1.71 respectively . The LOD and LOQ for Domperidone was found to be 1.49 , 4.5 respectively. This method was found to be good percentage recovery for Naproxen and Domperidone were found to be 98% and 101.6% respectively indicates that the proposed method was highly accurate. The specificity of method shows good correlation between retention times of standard with the sample. The method was extensively validated according to ICH guidelines for Linearity , Accuracy , Precision , Specificity , and Robustness .

scholarly journals Development and Validation of Stability-Indicating RP-HPLC Method for the Estimation of Lenalidomide and its Impurities in Oral Solid Dosage Form

2019 ◽  
Vol 35 (1) ◽  
pp. 140-149 ◽  
Author(s):  
Somana Siva Prasad ◽  
G. V. Krishna Mohan ◽  
A. Naga Babu
Keyword(s):  
Limit Of Detection ◽  
Degradation Products ◽  
Pharmaceutical Formulations ◽  
Hplc Method ◽  
Stability Robustness ◽  
Rp Hplc ◽  
Mobile Phases ◽  
Limit Of Quantitation ◽  
Successful Separation ◽  
Quality By Design Approach

In this study, a novel, simple and precise RP-HPLC method has been developed for the quantitative analysis of Lenalidomide (LLM) in pharmaceutical formulations using analytical quality by design approach. An X-bridge-C18 column (150 mm × 4.6 mm × 3.5 µ) with mobile phases containing a Potassium dihydrogen orthophosphate anhydrous buffer and methanol in the ratio of (90:10 v/v) and (35:65 v/v) are used for the estimation of LLM and its degradation products. The flow rate of 0.8 mL/min is maintained and all degradation studies are performed at 210 nm using photodiode array (PDA) detector. Method Validation is carried out according to International Council for Harmonisation (ICH) guidelines and the parameters namely; precision, accuracy, specificity, stability, robustness, linearity, limit of quantitation (LOQ) and limit of detection (LOD) are evaluated. The present developed RP-HPLC method shows the purity angle of peaks is less than their threshold angle, signifying that it to be suitable for stability studies. Hence, the developed method can be used for the successful separation of LLM and its impurities in the pharmaceutical dosage formulations.

Simultaneous Determination of Chloroquine and Pyrimethamine with Cetirizine in an Active Form and Human Serum by RP-HPLC

2021 ◽  
Author(s):  
Hina Shamshad ◽  
Ali Sayqal ◽  
Jahan Zeb ◽  
Agha Zeeshan Mirza
Keyword(s):  
Human Serum ◽  
Simultaneous Determination ◽  
Active Form ◽  
Internal Standard ◽  
Hplc Method ◽  
Serum Samples ◽  
Rp Hplc ◽  
Cetirizine Hydrochloride ◽  
Bulk Drug

Abstract A simple, accurate and precise RP-HPLC method was developed for the simultaneous determination of chloroquine, pyrimethamine and cetirizine hydrochloride concentrations in bulk drug and human serum. The assay was performed using a mobile phase of methanol: water (70:30) at pH of 2.8 ± 0.05 on the Purospher C-18 column with UV detection at 230 nm and rosuvastatin used as an internal standard. The retention times observed for chloroquine, pyrimethamine and cetirizine hydrochloride were 3.5, 2.5 and 5.5 minutes, respectively. The method was found to be specific for the assayed drugs showing a linear response in the concentration range of 1–100 μg mL−1 with coefficients of determination values of (r = 0.999). The method was developed and validated according to ICH guidelines. The method was used to monitor the serum samples and was found to be sensitive for therapeutic purposes, showing the potential to be a useful tool for routine analysis in laboratories.

Sign in / Sign up

Export Citation Format

Share Document