Safety of fungal biocontrol agent,Beauveria bassiana(Balsamo) Vuillemin to spider mite natural enemy,Stethorus punctillumWeise under field conditions

2015 ◽  
Vol 39 (4) ◽  
pp. 333
Author(s):  
monika Geroh ◽  
Rachna Gulati ◽  
kanika Tehri
Keyword(s):  
Beauveria Bassiana ◽  
Natural Enemy ◽  
Spider Mite ◽  
Biocontrol Agent ◽  
Field Conditions

scholarly journals Influence of Two Varieties of Broad Bean and Beauveria bassiana (Blas) on Aphis fabae scop. Under Field Conditions

2019 ◽  
Vol 9 (2) ◽  
pp. 16-19
Author(s):  
Zayoor Z. Omar ◽  
Tavga S. Rashid ◽  
Hayman K. Awla
Keyword(s):  
Beauveria Bassiana ◽  
Biocontrol Agent ◽  
Broad Bean ◽  
Field Conditions ◽  
Aphis Fabae ◽  
High Concentration ◽  
Black Bean ◽  
Local Variety ◽  
Potential Biocontrol Agent ◽  
Black Bean Aphid

Black bean aphid (Aphis fabae scop.), belongs to order Hemiptera and family Aphididae, is one of the important pests of the Fabaceae family. Beauveria bassiana is an important biocontrol agent to replace chemical insecticides. Therefore, in this research, two different varieties of broad bean with three different concentrations of B. bassiana were investigated on adults of fabae aphids under field conditions. After 3, 5, 7, and 14 days, the effect of the B. bassiana on the population of aphids was measured. The local variety was found to be more sensitive to the aphids compare with the Spain variety. In the high concentration (108 spores/ml), 80% mortality was obtained with B. bassiana in day 3 with local variety; then mortality increased after 5, 7, and 14 days of measuring the population of the adults. Mortality declined with the decrease in concentrations. The Spain variety was found to be resistance because fewer aphids were recorded compared to the local variety. In the highest concentration and day 14, mortality was 100%. The achieved results showed that B. bassiana can be used as a potential biocontrol agent for the management of black bean aphid in the fields.

Re-adaptation from alternative prey to target prey increased predation of predator on target mite

2019 ◽  
Vol 24 (3) ◽  
pp. 467 ◽  
Author(s):  
Jie Su ◽  
An-Di Zhu ◽  
Guo-Dong Han ◽  
Fang Dong ◽  
Jing Chen ◽  
...  
Keyword(s):  
Natural Enemy ◽  
Spider Mite ◽  
Biocontrol Agent ◽  
Mass Rearing ◽  
Predatory Mites ◽  
Alternative Prey ◽  
Tyrophagus Putrescentiae ◽  
Potential Biocontrol Agent ◽  
Tetranychus Turkestani

Neoseiulus bicaudus (Wainstein) is a natural enemy of spider mite and thrip and also a potential biocontrol agent for Tetranychus turkestani (Tetranychidae). Tyrophagus putrescentiae (Schrank) is an important alternative prey of N. bicaudus for mass rearing. We aimed to determine whether previous long-term feeding history, rearing on alternative prey versus target prey affected the performance of N. bicaudus. Therefore, the prey preference and functional response of predatory mites were compared between three strains: N. bicaudus fed on T. turkestani (TTS); N. bicaudus fed on T. putrescentiae (TPS); and N. bicaudus re-adapted from T. putrescentiae to T. turkestani (TPRS). When fed with the target prey, the preference rate and consumption of the TPS strain were significantly lower than the TTS strain. In addition, after 3-day-re-adaptation, the predatory mites (TPRS) increased their preference rate and consumption to target prey. The results suggested that re-adaptation before release could improve the ability of N. bicaudus to control its target prey.

scholarly journals Effects of local isolates of Beauveria bassiana (Balsamo) Vuillemin on the two-spotted spider mite, Tetranychus urticae (Koch) (Acari: Tetranychidae)

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Cenk Yucel
Keyword(s):  
Tetranychus Urticae ◽  
Beauveria Bassiana ◽  
Spider Mite ◽  
Lethal Effect ◽  
Probit Analysis ◽  
Plant Feeding ◽  
Wide Range ◽  
Two Spotted Spider Mite ◽  
Short Time ◽  
Tetranychus Urticae Koch

Abstract Background The two-spotted spider mite, Tetranychus urticae (Koch) (Acari: Tetranychidae), is a widely distributed plant-feeding pest that causes significant yield losses in a wide range of crops. Newly developed or improved environmentally friendly biocontrol agents serve as an alternative to traditional pest control tools. Experiment of the effects of 2 local fungal isolates of Beauveria bassiana (BGF14 and BCA32) was carried out against T. urticae under laboratory conditions. Results Both tested isolates had lethal effect in a short time after application, and this effect increased as time progressed. BGF14 and BCA32 isolates caused T. urticae mortality rates ranging from 25.88 to 61.92 and 32.36 to 62.03% when applied at the concentrations between 1×105 and 1×108 conidia/ml, respectively. According to the Probit analysis performed on the effect of fungi on T. urticae adults, the LC50 values of BGF14 and BCA32 isolates on the 7th day after inoculation were 2.6×106 and 6.3×104 conidia/ml, respectively, and the LT50 values for both fungi applied at a concentration of 108 conidia/ml were 2.14 and 2.23 days, respectively. Conclusions The 2 isolates of B. bassiana (BGF14 and BCA32) had the potentials to suppress T. urticae population and can be recommended as promising biocontrol agent candidates for control of T. urticae.

scholarly journals Application of Bio-Friendly Formulations of Chitinase-Producing Streptomyces cellulosae Actino 48 for Controlling Peanut Soil-Borne Diseases Caused by Sclerotium rolfsii

2021 ◽  
Vol 7 (3) ◽  
pp. 167
Author(s):  
Gaber Abo-Zaid ◽  
Ahmed Abdelkhalek ◽  
Saleh Matar ◽  
Mai Darwish ◽  
Muhammad Abdel-Gayed
Keyword(s):  
Open Field ◽  
Biocontrol Agent ◽  
Sclerotium Rolfsii ◽  
Dry Weight ◽  
Culture Broth ◽  
Field Conditions ◽  
Damping Off ◽  
Mycelium Growth ◽  
Particle Size Analyzer ◽  
Cell Pellet

Of ten actinobacterial isolates, Streptomyces cellulosae Actino 48 exhibited the strongest suppression of Sclerotium rolfsii mycelium growth and the highest chitinase enzyme production (49.2 U L−1 min−1). The interaction between Actino 48 and S. rolfsii was studied by scanning electron microscope (SEM), which revealed many abnormalities, malformations, and injuries of the hypha, with large loss of S. rolfsii mycelia density and mass. Three talc-based formulations with culture broth, cell-free supernatant, and cell pellet suspension of chitinase-producing Actino 48 were characterized using SEM, Fourier transform infrared spectroscopy (FTIR), and a particle size analyzer. All formulations were evaluated as biocontrol agents for reducing damping-off, root rot, and pods rot diseases of peanut caused by S. rolfsii under greenhouse and open-field conditions. The talc-based culture broth formulation was the most effective soil treatment, which decreased the percentage of peanut diseases under greenhouse and open-field conditions during two successive seasons. The culture broth formulation showed the highest increase in the dry weight of peanut shoots, root systems, and yielded pods. The transcriptional levels of three defense-related genes (PR-1, PR-3, and POD) were elevated in the culture broth formulation treatment compared with other formulations. Subsequently, the bio-friendly talc-based culture broth formulation of chitinase-producing Actino 48 could potentially be used as a biocontrol agent for controlling peanut soil-borne diseases caused by S. rolfsii.

scholarly journals Preservative Media for Beauveria Bassiana (bals.) Vuill Storage

2020 ◽  
Vol 23 (2) ◽  
pp. 1-6
Author(s):  
Helben Ismat Mohammaed ◽  
Mohammed Saeed Mirza ◽  
Feyroz Ramadan Hassan
Keyword(s):  
Beauveria Bassiana ◽  
Insecticidal Activity ◽  
Entomopathogenic Fungus ◽  
Biocontrol Agent ◽  
Insect Pests ◽  
Insect Pest ◽  
Effective Control ◽  
Effective Evaluation ◽  
Microbial Agent

Beauveria bassiana is an important entomopathogenic fungus that used as a biocontrol agent of insect pests. Maintaining and preserving B. bassiana cultures is essential for the effective evaluation of its potential as microbial agent against insect pest, for biodiversity studies and also for exchange of fungal material between laboratories. In the present work we evaluated the suitability of four preservation materials based on gelatin and rice to maintain the viability of B. bassiana to be used as baits for insect’s control. The gelatin amended with sugar recorded the maximum viability after 70 days of storage as 98.3% compared to 63.3% on rice media. Effective control of 80% of the ants was observed after 10 days of feeding on gelatin amended with sugar and rice bait. The results demonstrated that treatment with formulations containing conidia of B. bassiana presents insecticidal activity against ant in addition of acting as preservation materials.

Eco-friendly management of the pod borers Maruca vitrata (Fabricius) and Lampides boeticus (L.) of yard long bean under field conditions

ENTOMON ◽  
2020 ◽  
Vol 45 (4) ◽  
pp. 259-264
Author(s):  
V. Vineetha ◽  
B. Ramesha ◽  
K. M. Sreekumar ◽  
Madhu Subramanian ◽  
T. Santhosh Kumar
Keyword(s):  
Beauveria Bassiana ◽  
Vigna Unguiculata ◽  
Maruca Vitrata ◽  
Field Conditions ◽  
Pod Borer ◽  
Effective Treatments

Investigations on management of pod borers [Maruca vitrata (Fabricius), Lampides boeticus (L.)] of yard long bean (Vigna unguiculata subsp. sesquipedalis (L.) Verdcourt) under field conditions revealed that Spinosad 45 SC followed by Bt formulation 2× 108 cfu/ml and Beauveria bassiana @ 107 spores/ml of water were the most effective treatments in preventing pod borer infestation as well as controlling number of pod borer larvae.

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