scholarly journals Studies on virus diseases of fish. III. Morphological and experimental observations on the lymphocystis disease of the pike perch, Stizostedion vitreum

1939 ◽  
Vol 24 (8) ◽  
pp. 245-254
Author(s):  
Richard Weissenberg
Keyword(s):  
Stizostedion Vitreum ◽  
Virus Diseases ◽  
Pike Perch ◽  
Lymphocystis Disease

STUDIES ON CESTODES OF THE GENUS TRIAENOPHORUS FROM FISH OF LESSER SLAVE LAKE, ALBERTA: V. DESCRIPTION AND LIFE HISTORY OF TRIAENOPHORUS STIZOSTEDIONIS N.SP.

1945 ◽  
Vol 23d (5) ◽  
pp. 117-127 ◽  
Author(s):  
Richard B. Miller
Keyword(s):  
Life History ◽  
Male Genitalia ◽  
Sexual Maturity ◽  
Morphological Characters ◽  
Parietal Peritoneum ◽  
Stizostedion Vitreum ◽  
Pike Perch ◽  
History Of

Triaenophorus stizostedionis is a pseudophyllidean cestode that occurs as an adult in the intestine of the pike-perch, Stizostedion vitreum. It differs from T. crassus and T. nodulosus in several morphological characters but particularly in the shape of the scolex hooks and the size and disposition of the male genitalia. Sexual maturity is attained in the spring; spawning and death take place during the first two weeks of June. The eggs average 56 μ long by 40 μ wide. The coracidia average 73 by 71 μ. The procercoid develops in the copepod, Cyclops bicuspidatus, in from 10 days to two weeks. When fully grown it reaches 220 μ. The plerocercoids occur encysted on the visceral and parietal peritoneum of the trout-perch, Percopsis omiscomaycus. The life history is completed when an infested trout-perch is swallowed by a pike-perch.

Viruses Associated with Lymphocystis Disease and Dermal Sarcoma of Walleye (Stizostedion vitreum vitreum)

1976 ◽  
Vol 33 (11) ◽  
pp. 2408-2419 ◽  
Author(s):  
Tats Yamamoto ◽  
Richard D. Macdonald ◽  
Douglas C. Gillespie ◽  
Russell K. Kelly
Keyword(s):  
Electron Microscopic Examination ◽  
The Body ◽  
Stizostedion Vitreum ◽  
Electron Microscopic ◽  
Virus Particles ◽  
Large Numbers ◽  
Lymphocystis Disease ◽  
Spawning Run ◽  
Tumor Types ◽  
Dermal Sarcoma

Gross examination of a spawning run of walleye (Stizostedion vitreum vitreum) showed a large proportion of fish to have tumors on the body and fins that appeared to be characteristic of lymphocystis disease. Light and electron microscopic examination revealed the presence of two distinct tumor types. One was characteristic of lymphocystis, consisting of typical enlarged nonneoplastic cells surrounded by hyaline layers and containing many 260 nm diameter lymphocystis virus particles in the cytoplasm. The other tumor, referred to as a dermal sarcoma, consisted of a solid mass of normal-sized cells and contained in the cytoplasm large numbers of 135 nm diameter virus particles referred to as walleye dermal sarcoma (WDS) virus. The WDS virus was similar in appearance to the leukoviruses and, with its outer layer sectioned tangentially, exhibited symmetry like a member of a leukovirus group designated by Fenner as subgenus C.

Search mode for rapid detection of virus particles

1991 ◽  
Vol 49 ◽  
pp. 286-287
Author(s):  
O. E. Bradfute
Keyword(s):  
Electron Microscopy ◽  
Rapid Detection ◽  
Plant Virus ◽  
Low Dose ◽  
Search Mode ◽  
Virus Diseases ◽  
Virus Particles ◽  
Focus Image ◽  
Time Required ◽  
Rapid Switching

Electron microscopy is frequently used in preliminary diagnosis of plant virus diseases by surveying negatively stained preparations of crude extracts of leaf samples. A major limitation of this method is the time required to survey grids when the concentration of virus particles (VPs) is low. A rapid survey of grids for VPs is reported here; the method employs a low magnification, out-of-focus Search Mode similar to that used for low dose electron microscopy of radiation sensitive specimens. A higher magnification, in-focus Confirm Mode is used to photograph or confirm the detection of VPs. Setting up the Search Mode by obtaining an out-of-focus image of the specimen in diffraction (K. H. Downing and W. Chiu, private communications) and pre-aligning the image in Search Mode with the image in Confirm Mode facilitates rapid switching between Modes.

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