scholarly journals Transcription of pericentromeric tandemly repeated DNA transcripts in human preovulatory oocytes

2019 ◽  
Vol 35 (3) ◽  
pp. 207-208
Author(s):  
M. A. Dobrynin ◽  
A. S. Kalugina ◽  
N. M. Korchagina ◽  
A. Prjibelski ◽  
O. I. Podgornaya ◽  
...  

1991 ◽  
Vol 1 (2) ◽  
pp. 159-166 ◽  
Author(s):  
J. Maluszynska ◽  
J.S. Heslop-Harrison






1983 ◽  
Vol 165 (4) ◽  
pp. 567-585 ◽  
Author(s):  
Brenda S. Lam ◽  
Dana Carroll


Genome ◽  
1992 ◽  
Vol 35 (6) ◽  
pp. 1037-1044 ◽  
Author(s):  
Cort S. Madsen ◽  
Kevin P. McHugh ◽  
Siwo R. de Kloet

We have investigated the evolution of a 190 base pair tandemly repeated DNA sequence (RBMII) in 27 different species of waterfowl. In this paper we show that the RBMII sequence is present in many species belonging to 7 of the 11 Anatid tribes. Inter- and intra-tribal differences in repeat presence indicate that, although the RBMII sequence has been maintained among widely divergent species, it is rapidly evolving. Restriction enzyme analyses suggest very different hierarchical repeat organizations among different species. DNA sequence comparisons of 32 cloned monomer units from five different species revealed what appears to be a nonrandom distribution of sequence divergence, as well as large differences (up to 25-fold) in intraspecific sequence variation between relatively closely related species.Key words: repeated DNA, Anatidae, sequence variation.



Genome ◽  
1996 ◽  
Vol 39 (2) ◽  
pp. 243-248 ◽  
Author(s):  
Thomas Schmidt ◽  
Jörg Kudla

Monomers of a major family of tandemly repeated DNA sequences of Antirrhinum majus have been cloned and characterized. The repeats are 163–167 bp long, contain on average 60% A + T residues, and are organized in head-to-tail orientation. According to site-specific methylation differences two subsets of repeating units can be distinguished. Fluorescent in situ hybridization revealed that the repeats are localized at centromeric regions of six of the eight chromosome pairs of A. majus with substantial differences in array size. The monomeric unit shows no homologies to other plant satellite DNAs. The repeat exists in a similar copy number and conserved size in the genomes of six European species of the genus Antirrhinum. Tandemly repeated DNA sequences with homology to the cloned monomer were also found in the North American section Saerorhinum, indicating that this satellite DNA might be of ancient origin and was probably already present in the ancestral genome of both sections. Key words : Antirrhinum majus, satellite DNA, repetitive DNA, methylation, in situ hybridization.



Genomics ◽  
1989 ◽  
Vol 5 (1) ◽  
pp. 153-156 ◽  
Author(s):  
Ulrich Müller ◽  
Marc Lalande ◽  
Timothy A. Donlon ◽  
Michael W. Heartlein


Genome ◽  
2001 ◽  
Vol 44 (3) ◽  
pp. 463-469 ◽  
Author(s):  
Junqi Song ◽  
Fenggao Dong ◽  
Jason W Lilly ◽  
Robert M Stupar ◽  
Jiming Jiang

The cloning and propagation of large DNA fragments as bacterial artificial chromosomes (BACs) has become a valuable technique in genome research. BAC clones are highly stable in the host, Escherichia coli, a major advantage over yeast artificial chromosomes (YACs) in which recombination-induced instability is a major drawback. Here we report that BAC clones containing tandemly repeated DNA elements are not stable and can undergo drastic deletions during routine library maintenance and DNA preparation. Instability was observed in three BAC clones from sorghum, rice, and potato, each containing distinct tandem repeats. As many as 46% and 74% of the single colonies derived from a rice BAC clone containing 5S ribosomal RNA genes had insert deletions after 24 and 120 h of growth, respectively. We also demonstrated that BAC insert rearrangement can occur in the early stage of library construction and duplication. Thus, a minimum growth approach may not avoid the instability problem of such clones. The impact of BAC instability on genome research is discussed.Key words: repetitive DNA, large insert DNA library, genome research.



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