scholarly journals An In Vivo Evaluation of the Effect of Repeated Administration and Clearance of Targeted Contrast Agents on Molecular Imaging Signal Enhancement

Theranostics ◽  
2013 ◽  
Vol 3 (2) ◽  
pp. 93-98 ◽  
Author(s):  
Jason E. Streeter ◽  
Paul A. Dayton
Keyword(s):  
Molecular Imaging ◽  
Contrast Agents ◽  
Repeated Administration ◽  
Signal Enhancement ◽  
In Vivo Evaluation ◽  
Targeted Contrast Agents

In vivo Evaluation and Alzheimer’s Disease Treatment Outcome of siRNA Loaded Dual Targeting Drug Delivery System

2019 ◽  
Vol 20 (1) ◽  
pp. 56-62 ◽  
Author(s):  
Chi Zhang ◽  
Zhichun Gu ◽  
Long Shen ◽  
Xianyan Liu ◽  
Houwen Lin
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Treatment Outcome ◽  
Neuroprotective Effect ◽  
Sirna Delivery ◽  
Repeated Administration ◽  
Spatial Learning And Memory ◽  
In Vivo Evaluation

Background: To deliver drugs to treat Alzheimer’s Disease (AD), nanoparticles should firstly penetrate through blood brain barrier, and then target neurons. Methods: Recently, we developed an Apo A-I and NL4 dual modified nanoparticle (ANNP) to deliver beta-amyloid converting enzyme 1 (BACE1) siRNA. Although promising in vitro results were obtained, the in vivo performance was not clear. Therefore, in this study, we further evaluated the in vivo neuroprotective effect and toxicity of the ANNP/siRNA. The ANNP/siRNA was 80.6 nm with good stability when incubated with serum. In vivo, the treatment with ANNP/siRNA significantly improves the spatial learning and memory of APP/PS1 double transgenic mice, as determined by mean escape latency, times of crossing the platform area during the 60 s swimming and the percentage of the distance in the target quadrant. Results and Conclusion: After the treatment, BACE1 RNA level of ANNP/siRNA group was greatly reduced, which contributed a good AD treatment outcome. Finally, after repeated administration, the ANNP/siRNA did not lead to significant change as observed by HE staining of main organs, suggesting the good biocompatibility of ANNP/siRNA. These results demonstrated that the ANNP was a good candidate for AD targeting siRNA delivery.

scholarly journals Polysialic Acid Modified Liposomes for Improving Pharmacokinetics and Overcoming Accelerated Blood Clearance Phenomenon

Coatings ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 834
Author(s):  
Xi Han ◽  
Ting Zhang ◽  
Mengyang Liu ◽  
Yanzhi Song ◽  
Xinrong Liu ◽  
...  
Keyword(s):  
Blood Circulation ◽  
Polysialic Acid ◽  
Repeated Administration ◽  
The Body ◽  
Circulation Time ◽  
Blood Clearance ◽  
In Vivo Evaluation ◽  
Accelerated Blood Clearance ◽  
Abc Phenomenon

Poly (ethylene glycol) (PEG) modified nanocarriers are being used widely in the drug delivery system (DDS). However, the “accelerated blood clearance (ABC) phenomenon” was induced upon repeated administration of PEG-modified liposomes, resulting in reduced blood circulation time, and increased accumulation in liver and spleen. To avoid the unexpected phenomenon, polysialic acid (PSA) was selected to modify liposomes. PSA is a natural, highly hydrophilic polysaccharide polymer for which no receptors exists in the body. It is non-immunogenic, biodegradable and endows the conjugated bioactive macromolecule and drugs with increased circulation time in vivo. In the present study, the in vivo evaluation showed that PSA modified liposomes (PSA-Lip) afford extended blood circulation in wistar rats and beagle dogs. Moreover, the ABC phenomenon did not occur and the IgM antibody was not induced after repeated injections of PSA-Lip. These results strongly suggest that PSA modification represents a promising strategy to afford good stealth of the liposomes without evoking the ABC phenomenon.

scholarly journals Molecular imaging perspectives

2005 ◽  
Vol 2 (3) ◽  
pp. 133-144 ◽  
Author(s):  
Paul J Cassidy ◽  
George K Radda
Keyword(s):  
Molecular Imaging ◽  
Contrast Agents ◽  
Physical Science ◽  
Life Science ◽  
Interdisciplinary Collaboration ◽  
Nuclear Imaging ◽  
Imaging Modalities ◽  
Molecular Events ◽  
Biological Target

Molecular imaging is an emerging technology at the life science/physical science interface which is set to revolutionize our understanding and treatment of disease. The tools of molecular imaging are the imaging modalities and their corresponding contrast agents. These facilitate interaction with a biological target at a molecular level in a number of ways. The diverse nature of molecular imaging requires knowledge from both the life and physical sciences for its successful development and implementation. The aim of this review is to introduce the subject of molecular imaging from both life science and physical science perspectives. However, we will restrict our coverage to the prominent in vivo molecular imaging modalities of magnetic resonance imaging, optical imaging and nuclear imaging. The physical basis of these imaging modalities, the use of contrast agents and the imaging parameters of sensitivity, temporal resolution and spatial resolution are described. Then, the specificity of contrast agents for targeting and sensing molecular events, and some applications of molecular imaging in biology and medicine are given. Finally, the diverse nature of molecular imaging and its reliance on interdisciplinary collaboration is discussed.

Holmium phosphate nanoparticles as negative contrast agents for high-field magnetic resonance imaging: Synthesis, magnetic relaxivity study and in vivo evaluation

2021 ◽  
Vol 587 ◽  
pp. 131-140
Author(s):  
Elisabet Gómez-González ◽  
Carlos Caro ◽  
Diego Martínez-Gutiérrez ◽  
María L. García-Martín ◽  
Manuel Ocaña ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Contrast Agents ◽  
High Field ◽  
Negative Contrast ◽  
Resonance Imaging ◽  
In Vivo Evaluation

scholarly journals Radiolabeled Molecular Imaging Probes for the In Vivo Evaluation of Cellulose Nanocrystals for Biomedical Applications

2018 ◽  
Vol 20 (2) ◽  
pp. 674-683 ◽  
Author(s):  
Surachet Imlimthan ◽  
Sofia Otaru ◽  
Outi Keinänen ◽  
Alexandra Correia ◽  
Kalle Lintinen ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Cellulose Nanocrystals ◽  
Biomedical Applications ◽  
In Vivo Evaluation ◽  
Molecular Imaging Probes ◽  
Imaging Probes

A modified commercial ultrasound scanner used for in vivo photoacoustic imaging of nude mice injected with non-targeted contrast agents

2008 ◽  
Author(s):  
Ladislav Jankovic ◽  
Khalid Shahzad ◽  
Yao Wang ◽  
Michael Burcher ◽  
Frank-Detlef Scholle ◽  
...  
Keyword(s):  
Contrast Agents ◽  
Nude Mice ◽  
Photoacoustic Imaging ◽  
Targeted Contrast Agents ◽  
Ultrasound Scanner

DEVELOPMENT OF NEEDLE-BASED MICROENDOSCOPY FOR FLUORESCENCE MOLECULAR IMAGING OF BREAST TUMOR MODELS

2009 ◽  
Vol 02 (04) ◽  
pp. 343-352
Author(s):  
CHAO-WEI CHEN ◽  
TIFFANY R. BLACKWELL ◽  
RENEE NAPHAS ◽  
PAUL T. WINNARD ◽  
VENU RAMAN ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Molecular Imaging ◽  
Contrast Agents ◽  
Breast Biopsy ◽  
Fluorescent Imaging ◽  
Model Systems ◽  
Breast Cancers ◽  
Imaging Device ◽  
Fluorescence Molecular Imaging

Fluorescence molecular imaging enables the visualization of basic molecular processes such as gene expression, enzyme activity, and disease-specific molecular interactions in vivo using targeted contrast agents, and therefore, is being developed for early detection and in situ characterization of breast cancers. Recent advances in developing near-infrared fluorescent imaging contrast agents have enabled the specific labeling of human breast cancer cells in mouse model systems. In synergy with contrast agent development, this paper describes a needle-based fluorescence molecular imaging device that has the strong potential to be translated into clinical breast biopsy procedures. This microendoscopy probe is based on a gradient-index (GRIN) lens interfaced with a laser scanning microscope. Specifications of the imaging performance, including the field-of-view, transverse resolution, and focus tracking characteristics were calibrated. Orthotopic MDA-MB-231 breast cancer xenografts stably expressing the tdTomato red fluorescent protein (RFP) were used to detect the tumor cells in this tumor model as a proof of principle study. With further development, this technology, in conjunction with the development of clinically applicable, injectable fluorescent molecular imaging agents, promises to perform fluorescence molecular imaging of breast cancers in vivo for breast biopsy guidance.

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