scholarly journals Occurrence of cucurbit viruses on field-grown melon and watermelon in the Thrace region of Turkey

2007 ◽  
Vol 87 (3) ◽  
pp. 123-130 ◽  
Author(s):  
Gassan Köklü ◽  
Özgür Yilmaz
Keyword(s):  
Mosaic Virus ◽  
Infection Type ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Spot Virus ◽  
Serological Tests ◽  
Polyclonal Antisera ◽  
Thrace Region

Abstract A survey for the detection of cucumber mosaic virus (CMV), papaya ringspot virus-W (PRSV-W), squash mosaic virus (SqMV), melon necrotic spot virus (MNSV), cucumber green mottle mosaic virus (CGMMV), zucchini yellow mosaic virus (ZYMV) and watermelon mosaic virus-2 (WMV-2) was carried out in June and July 2005, covering 17 melon fields and 19 watermelon fields in the Tekirdag, Edirne and Kırklareli provinces of Turkish Thrace. Among all the fields sampled, only one melon field was not virus-infected. In all, 502 melon and watermelon samples were tested for the presence of seven viruses with ELISA tests using polyclonal antisera. Overall, 333 out of 502 samples tested positive for the investigated viruses: 167 out of 235 plant samples in Tekirdag, 103 out of 187 samples in Edirne, and 63 out of 80 samples in Kırklareli were positive. Serological tests showed that six out of the seven tested viruses were present in the Thrace region of Turkey. The following rates of incidence of tested viruses on watermelon were found: ZYMV (45.5%), WMV-2 (34.2%), CMV (19.9%), PRSV-W (2.1%), SqMV (1.8%) and MNSV (0.4%), while the rates of incidence on melon were ZYMV (40.3%), WMV-2 (31.2%), CMV (7.2%), PRSV-W (2.3%), SqMV (0.5%) and MNSV (1.8%). The WMV-2+ZYMV mixed infection type was the most widespread both on melon and on watermelon samples at 16.7% and 11.4%, respectively.

scholarly journals First Report of Papaya ringspot virus and Zucchini yellow mosaic virus in Luohanguo (Siraitia grosvenorii) in China

Plant Disease ◽  
2005 ◽  
Vol 89 (5) ◽  
pp. 530-530 ◽  
Author(s):  
Y.-M. Liao ◽  
X.-J. Gan ◽  
B. Chen ◽  
J.-H. Cai
Keyword(s):  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Viral Disease ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Rt Pcr ◽  
First Report ◽  
Siraitia Grosvenorii

Luohanguo, Siraitia grosvenorii (Swingle) C. Jeffrey, is a perennial cucurbitaceous plant that is an economically important medicinal and sweetener crop in Guangxi province, China. Surveys conducted during the summer to fall seasons of 2003-2004 in northern Guangxi showed symptoms typical of a viral disease, including leaf mottling, mosaic, vein clearing, curling, and shoestring-like distortion in the field. Mechanical inoculation of sap from leaves of symptomatic plants collected from the surveyed areas caused similar symptoms on tissue culture-derived healthy Luohanguo plants. Two sequences of 0.7 and 1.6 kb with 88 and 97% identity to Papaya ringspot virus (PRSV) and Zucchini yellow mosaic virus (ZYMV) were amplified using reverse transcription-polymerase chain reaction (RT-PCR) with purified flexuous viral particles or total RNA extracted from the symptomatic Luohanguo leaves as templates with conserved degenerate potyvirus primers (1). To confirm the results, primers specific for PRSV (PP1/PP2, genome coordinates 4064-4083/5087-5069, GenBank Accession No X97251) and ZYMV (ZP1/ZP2, genome coordinates 5540-5557/7937-7920, GenBank Accession No L31350) were used to perform RT-PCR from the same RNA templates. The expected 1.0- and 2.3-kb fragments were amplified and they were 90 and 95% identical to PRSV and ZYMV in sequence, respectively. Watermelon mosaic virus was not detected. To our knowledge, this is the first report of the occurrence of PRSV and ZYMV in Luohanguo. Reference: (1) A. Gibbs et al. J. Virol. Methods 63:9, 1997.

scholarly journals Yield and Virus Resistance of Summer Squash Cultivars and Breeding Lines in North Carolina

1998 ◽  
Vol 8 (1) ◽  
pp. 31-39 ◽  
Author(s):  
Jonathan R. Schultheis ◽  
S. Alan Walters
Keyword(s):  
North Carolina ◽  
Mosaic Virus ◽  
Virus Resistance ◽  
Zucchini Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Breeding Lines ◽  
Summer Squash

Yellow and zucchini squash (Cucurbita pepo L.) cultigens (breeding lines and cultivars) were evaluated over a 2-year (1995 and 1996) period in North Carolina. Yellow squash cultigens that performed well (based on total marketable yields) were `Destiny III', `Freedom III', `Multipik', XPHT 1815, and `Liberator III' in Fall 1995 and HMX 4716, `Superpik', PSX 391, `Monet', `Dixie', XPH 1780, and `Picasso' in Spring 1996. Some of the yellow squash cultigens evaluated had superior viral resistance: XPHT 1815, XPHT 1817, `Freedom III', `Destiny III', `Freedom II', TW 941121, `Prelude II', and `Liberator III' in Fall 1995 and XPHT 1815, `Liberator III', `Prelude II', and `Destiny III' in Fall 1996; all these cultigens were transgenic. The yellow squash cultigens that performed well (based on total marketable yields) in the Fall 1995 test had transgenic virus resistance (`Destiny III', `Freedom III', XPHT 1815, and `Liberator III') or had the Py gene present in its genetic background (`Multipik'). Based on total marketable yields, the best zucchini cultigens were XPHT 1800, `Tigress', XPHT 1814, `Dividend' (ZS 19), `Elite', and `Noblesse' in Fall 1995; and `Leonardo', `Tigress', `Hurricane', `Elite', and `Noblesse' in Spring 1996. The zucchini cultigens with virus resistance were TW 940966, XPHT 1814, and XPHT 1800 in Fall 1995 and XPHT 1800, XPHT 1776, XPHT 1777, XPHT 1814, and XPHT 1784 in Fall 1996. Even though TW 940966 had a high level of resistance in the Fall 1995 test, it was not as high yielding as some of the more susceptible lines. Viruses detected in the field were papaya ringspot virus (PRSV) and watermelon mosaic virus (WMV) for Fall 1995; while PRSV, zucchini yellow mosaic virus (ZYMV), and WMV were detected for Fall 1996. Summer squash cultigens transgenic for WMV and ZYMV have potential to improve yield, especially during the fall when viruses are more prevalent. Most transgenic cultigens do not possess resistance to PRSV, except XPHT 1815 and XPHT 1817. Papaya ringspot virus was present in the squash tests during the fall of both years. Thus, PRSV resistance must be transferred to the transgenic cultigens before summer squash can be grown during the fall season without the risk of yield loss due to viruses.

scholarly journals Seleção de acessos e progênies de Citrullus spp. para resistência a três potyvirus

2005 ◽  
Vol 30 (4) ◽  
pp. 394-399 ◽  
Author(s):  
Lindomar M. da Silveira ◽  
Manoel A. de Queiróz ◽  
J. Albérsio de A. Lima ◽  
Maria Z. de Negreiros ◽  
Najara F. Ramos ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus

Visando selecionar acessos e progênies de melancia (Citrullus spp.) como fontes de resistência aos potyvirus: Papaya ringspot virus tipo watermelon (PRSV-W), Watermelon mosaic virus (WMV) e Zucchini yellow mosaic virus (ZYMV), oito genótipos foram avaliados, sendo seis dos acessos (87-019, 87-029, 91-080, PI-244018, 91-043 e PI-195927) e dois do acesso PI-244019 (PI-244019A e PI-244019B) do Banco Ativo de Germoplasma (BAG) de cucurbitáceas do Nordeste brasileiro, da Embrapa Semi-Árido em Petrolina-PE. Também foram avaliadas progênies endogâmicas e de polinização livre derivadas desses acessos. As avaliações foram realizadas em de casa de vegetação, mediante inoculações mecânicas, e avaliação por Elisa, no Laboratório de Virologia Vegetal da UFC. As plantas não infetadas foram selecionadas e cultivadas na Estação Experimental de Bebedouro na Embrapa Semi-Árido em Petrolina-PE, onde ocorreram inoculações naturais de vírus por vetores. Foram constatadas plantas não infetadas com o PRSV-W nos acessos 87-019, PI-244019A, 91-080, PI-244018, PI-244019B e PI-195927; plantas não infetadas com o WMV nos acessos 87-019 e 87-029 e plantas não infetadas com o ZYMV nos acessos PI-244019A, 87-029, 91-080, 91-043, PI-244019B e PI-195927. As progênies apresentaram comportamento diferenciado, com percentagem de plantas selecionadas variando de 20 a 100% nas progênies avaliadas para resistência a PRSV-W e 60 a 100% nas progênies avaliadas para resistência a WMV. Nenhuma das progênies testadas apresentou resistência ao ZYMV, evidenciando possível diferença entre a resistência ao PRSV-W e ao WMV apresentada nas progênies e a resistência apresentada ao ZYMV, visto que as progênies foram submetidas ao mesmo número de autofecundações.

scholarly journals Inheritance of Resistance to the Watermelon Strain of Papaya Ringspot Virus in the Cucumber Line TMG-1

HortScience ◽  
1995 ◽  
Vol 30 (2) ◽  
pp. 338-340 ◽  
Author(s):  
T. Wai ◽  
R. Grumet
Keyword(s):  
Mosaic Virus ◽  
Dominant Gene ◽  
Zucchini Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Genetics Of Resistance ◽  
Elisa Data

The inbred cucumber (Cucumis sativus L.) line TMG-1 is resistant to three potyviruses: zucchini yellow mosaic virus (ZYMV), watermelon mosaic virus (WMV), and the watermelon strain of papaya ringspot virus (PRSV-W). In this study we sought to determine the genetics of resistance to PRSV-W. TMG-1 was crossed with WI-2757, an inbred line susceptible to all three viruses. Segregation data indicated that resistance to PRSV-W was due to a single dominant gene (proposed designation, Prsv-2). Enzyme-linked immunosorbent assay (ELISA) data suggested that the mechanism of resistance to PRSV-W differs from that for ZYMV and WMV, and may be better described as tolerance. Although the plants were free of symptoms, high PRSV-W titers existed in young expanding leaves of the TMG-1 plants and the WI-2757 × TMG-1 F1 progeny.

scholarly journals GENETIC RELATIONSHIPS AMONG RESISTANCE TO ZUCCHINI YELLOW MOSAIC VIRUS, WATERMELON MOSAIC VIRUS, PAPAYA RINGSPOT VIRUS, AND POWDERY MILDEW IN MELON (CUCUMIS MELO)

HortScience ◽  
1996 ◽  
Vol 31 (6) ◽  
pp. 913G-914
Author(s):  
Konstantinos Anagnostou ◽  
Molly Kyle ◽  
Rafael Perl-Treves
Keyword(s):  
Powdery Mildew ◽  
Mosaic Virus ◽  
Cucumis Melo ◽  
Genetic Relationships ◽  
Zucchini Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Relationship Of

We have studied the relationship of resistance to watermelon mosaic virus (WMV), zucchini yellow mosaic virus (ZYMV), papaya ringspot virus (PRSV), and powdery mildew (PM) in melon (Cucumis melo). We have confirmed monogenic dominant inheritance of these four resistances and report that PI414723-4S3, which was initially selected as a source of ZYMR, is also a source of dominant monogenic resistance to PRSV. Further, we observed departure from independent assortment for resistance to WMV and ZYMV in a study of 73 (UC Top Mark × PI414723-4S3) F3 families (χ2 = 39.87 significant at both 0.01 and 0.05 levels), indicating linkage between Wmv and Zym. The map distance between these resistance genes calculated from the number of recombinant families (RF% = 9.58) was 10.5 cM. Compari-sons among WMV, PM, ZYMV-PM, PRSV-PM, ZYMV-PRSV, and WMV-PRSV of 48 (TM × PI414723-4S3) F3 families, which were screened with all four pathogens, showed no consistent cosegregation.

A sensitive semi-quantitative biotin-streptavidin ELISA for the detection of potyviruses infecting cucurbits

1991 ◽  
Vol 42 (3) ◽  
pp. 417 ◽  
Author(s):  
RG Dietzgen ◽  
ME Herrington
Keyword(s):  
Mosaic Virus ◽  
Sandwich Elisa ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Test Plate ◽  
Breeding Lines ◽  
Elisa System ◽  
Enzyme Conjugate

The biotin-streptavidin (BA)-ELISA system was optimized for the detection of three potyviruses infecting cucurbits, the watermelon strain of papaya ringspot virus (PRSV-W), zucchini yellow mosaic virus (ZYMV) and watermelon mosaic virus 2. BA-ELISA was four to eight times more sensitive than double antibody sandwich ELISA and detected all three viruses in the nanogram range. BA-ELSA was virus-specific but did not differentiate between different pathotypes of ZYMV. The use of egg-white avidin-enzyme conjugate or the simultaneous incubation of biotinylated antibody and streptavidin-enzyme conjugate decreased the sensitivity of BA-ELISA. The concentrations of PRSV-W and ZYMV in field-infected cucurbit specimens or in resistant breeding lines were estimated from calibration curves derived from a dilution series of purified virus in extracts of uninfected plants on each test plate. Thus it was possible to determine semi-quantitatively the degree and uniformity of resistance in plants from these lines and to differentiate resistant from tolerant selections.

scholarly journals Identificação sorológica de espécies de vírus que infetam cucurbitáceas em áreas produtoras do Maranhão

2001 ◽  
Vol 26 (1) ◽  
pp. 90-92 ◽  
Author(s):  
MARIA C. C. L. MOURA ◽  
J. ALBERSIO A. LIMA ◽  
VANÚZIA B. OLIVEIRA ◽  
M. FÁTIMA B. GONÇALVES
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Immunosorbent Assay ◽  
Citrullus Lanatus ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Cucurbita Moschata

Os vírus representam sérios obstáculos para o sucesso da olericultura no mundo inteiro, constituindo a identificação daqueles de maior incidência numa região, papel fundamental para o estabelecimento de estratégias de controle. Visitas de campo foram realizadas a plantios de espécies de cucurbitáceas em áreas produtoras do Maranhão e amostras foliares foram coletadas de 118 plantas com sintomas ou suspeita de sintomas de vírus, sendo 46 de abóbora (Cucurbita moschata), 30 de melancia (Citrullus lanatus), 23 de maxixe (Cucumis anguria), 13 de pepino (C. sativus) e seis de melão (C. melo). Todas as amostras foram testadas contra anti-soros específicos para os principais vírus das famílias Bromoviridae, Comoviridae e Potyviridae que infetam cucurbitáceas no Nordeste, mediante "enzyme-linked immunosorbent assay" (ELISA) indireto e dupla difusão em agar. Os resultados revelaram a identificação sorológica de Papaya ringspot vírus (PRSV) em 64,4% das amostras analisadas, seguido de Watermelon mosaic virus-2 (WMV-2) em 15,2%, Cucumber mosaic virus (CMV) em 6,8%, Squash mosaic virus (SqMV) em 3,4% e Zucchini yellow mosaic virus (ZYMV) em 3,4%. Este levantamento confirma a predominância do PRSV em espécies de cucurbitáceas cultivadas no estado do Maranhão.

scholarly journals Squash Yellow Leaf Curl Virus: A New Whitefly-Transmitted Poty-Like Virus

Plant Disease ◽  
1998 ◽  
Vol 82 (5) ◽  
pp. 475-478 ◽  
Author(s):  
A. A. Zouba ◽  
M. V. Lopez ◽  
H. Anger
Keyword(s):  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Yellow Leaf ◽  
Serological Tests ◽  
Thin Sections ◽  
Leaf Curl Virus ◽  
Leaf Curl

A severe yellow and leaf curl disease affecting field squash was found in the Batinah region of the Sultanate of Oman. The symptoms appear as small yellow spots, diffuse veinal yellowing, and leaf curling of young leaves. The inciting virus was easily transmitted by mechanical inoculation and by the whitefly Bemisia tabaci in a semi-persistent manner. The host range of the virus was restricted to two cucurbit species. Leaf dip preparations contained few flexuous particles about 700 to 750 nm long. Pinwheel-like inclusion bodies were observed in thin sections of diseased squash tissues. Serological tests by enzyme-linked immunosorbent assay showed that the virus is serologically related to watermelon mosaic virus-2, but not to zucchini yellow mosaic virus or papaya ring spot virus (watermelon strain). In view of these properties, this virus is considered to be a newly described virus and is tentatively named squash yellow leaf curl virus.

scholarly journals Ocorrência de patógenos em cultivos de melancia e abóbora no sertão da Paraíba

2016 ◽  
Vol 11 (1) ◽  
pp. 07 ◽  
Author(s):  
M. G. F. O. Soares ◽  
J. A. Soares ◽  
M. A. Cezar ◽  
T. A. L. Cardoso ◽  
J. A. A. Lima
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Morphological Characteristics ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Mixed Infections ◽  
Spot Virus ◽  
Papaya Ring Spot Virus ◽  
Ring Spot

<p>Por constituírem uma importante fonte de alimento,<strong> </strong>plantações de melancia e abóbora são comumente cultivadas no sertão paraibano, porém pouco se sabe sobre a ocorrência de patógenos causadores de doenças, os quais limitam a sua produtividade e renda aos produtores. Visando obter informações sobre a ocorrência dos patógenos virais e fúngicos em cultivos de abóbora e melancia situados em municípios produtores no sertão da Paraíba, amostras coletadas com sintomas de mosaico e deformação foliar, típicos de doenças virais foram analisadas pela técnica sorológica “enzime linked immuno sorbentassay” (Elisa) indireto para <em>Papaya ring spot virus, </em>type watermelon (PRSV-W), <em>Watermelon mosaic virus </em>(WMV), <em>Zucchini yellow mosaic virus </em>(ZYMV) e <em>Cucumber mosaic virus </em>(CMV). O teste de dupla difusão em Agar foi utilizado para verificar a presença de <em>Squash mosaic virus </em>(SqMV). Em contrapartida, amostras coletadas com sintomas de doenças fúngicas foram analisadas pelo isolamento do patógeno e visualização de suas características morfológicas em microscópio óptico. Em abóbora, houve prevalência dos vírus ZYMV e PRSV-W em infecções simples e mistas, e maior incidência dos fungos <em>Cladosporium</em> spp., e <em>Alternaria</em> spp. Em melancia detectou-se<em> </em>infecção simples e mistas das espécies PRSV-W, WMV e ZYMV, e maior freqüência de <em>Fusarium</em> spp. e <em>Alternaria</em> spp.. Não foram detectados os vírus CMV e SqMV. Os resultados obtidos revelam a ocorrência de vários patógenos fúngicos e viróticos em cultivos de abóbora e melancia situados no sertão da Paraíba e ressaltam a importância da utilização de estratégias de manejo que reduzem os danos ocasionados por esses patógenos.</p><p align="center"><strong><em>Occurrence of pathogens in watermelon and pumpkin crops in the State of Paraiba</em></strong></p><p><strong>Abstract:</strong> By constitute an important food source, watermelon and pumpkin plantations are commonly grown on Paraíba<strong> </strong>backlands, but little is known about the occurrence of disease-causing pathogens, which limit their productivity and income to producers. With objective to get information about the viral and fungal pathogens occurrence in pumpkin and watermelon crops in producing counties located in the backlands of Paraiba, samples with symptoms of mosaic and leaf distortion, typical of viral etiology of disease were collected and analyzed by "Enzyme linked immune sorbentassay" (Elisa) Indirect for species <em>Papaya ring spot virus</em>, type watermelon (PRSV-W), <em>Watermelon mosaic virus </em>(WMV), <em>Zucchini yellow mosaic virus</em> ( ZYMV) and <em>Cucumber mosaic virus</em> (CMV). The double diffusion test in Agar was used to verify the presence of <em>Squash mosaic virus</em> (SqMV). In contrast, samples collected with fungal disease symptoms were analyzed based on the isolation of the pathogen and viewing their morphological characteristics under an optical microscope. In pumpkin, there was prevalence of ZYMV and PRSV-W in single and mixed infections, and higher incidence of <em>Cladosporium</em> spp. and <em>Alternaria</em> spp. On the other hand, in watermelon was detected single and mixed infections of PRSV-W, WMV and ZYMV species, and higher frequency of <em>Fusarium </em>spp. and <em>Alternaria</em> spp.. Were not detected the virus CMV and SqMV. The results show the occurrence of various fungal and viral pathogens in pumpkin and watermelon crops located in the backlands of Paraiba and emphasize the importance of using management strategies that reduce the damage caused by these pathogens.</p>

scholarly journals Identificação sorológica de espécies de potyvirus em melancia, no estado de Roraima

2004 ◽  
Vol 29 (6) ◽  
pp. 687-689 ◽  
Author(s):  
Bernardo A. Halfeld-Vieira ◽  
Najara F. Ramos ◽  
Francisco A.C. Rabelo Filho ◽  
M. Fátima B. Gonçalves ◽  
Katia L. Nechet ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Immunosorbent Assay ◽  
Citrullus Lanatus ◽  
Zucchini Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus

No período de maio de 2003 a março de 2004, foram coletadas amostras foliares de plantas de melancia (Citrullus lanatus) de 21 campos de cultivo de cucurbitáceas, no Estado de Roraima. As amostras exibiam diferentes sintomas de vírus e foram levadas para o Laboratório de Virologia Vegetal da Universidade Federal do Ceará para serem testadas por "enzyme linked immunosorbent assay" (Elisa)-indireto, contra anti-soros específicos para Cucumber mosaic virus (CMV), Papaya ringspot virus estirpe melancia (PRSV-W), Watermelon mosaic virus (WMV) e Zucchini yellow mosaic virus (ZYMV). Nos testes de Elisa, utilizou-se o conjugado universal, anti-imunoglobulina (IgG) de coelho produzida em cabra conjugada à enzima fosfatase alcalina. Todas as amostras foram testadas, também, por dupla difusão contra o anti-soro para Squash mosaic virus (SqMV). Os resultados indicaram a presença do PRSV-W em 84,2% das amostras coletadas em maio de 2003, em 7,1% das amostras coletadas em dezembro de 2003 e em 55,6% das amostras coletadas em março de 2004. A presença do ZYMV foi observada em 10,5% das amostras coletadas em maio de 2003, 21,4% das amostras coletadas em dezembro de 2003 e em 25,9% das amostras de março de 2004. O WMV foi detectado somente em oito das amostras coletadas em março de 2004 (29,6%). Os resultados desta pesquisa confirmam a ampla dispersão do PRSV-W em cultivos de cucurbitáceas no território brasileiro e a preocupante expansão do ZYMV em razão dos elevados prejuízos que o mesmo tem causado em outras partes do mundo.

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