scholarly journals A note on four nonradioactive labeling systems for dot hybridization detection of potato viruses

2005 ◽  
Vol 72 (2) ◽  
pp. 81-86 ◽  
Author(s):  
P. Audy ◽  
J.-G. Parent ◽  
A. Asselin
Keyword(s):  
Nucleic Acid ◽  
Plant Virus ◽  
Colorimetric Detection ◽  
Dna Probes ◽  
Genomic Rnas ◽  
Complementary Dna ◽  
Dot Hybridization ◽  
Labeled Probe ◽  
Hybridization Detection ◽  
Nonradioactive Labeling

Complementary DNA clones of genomic RNAs of potato (Solarium tuberosum) viruses S (PVS), X (PVX) and Y (PVY) were produced and tested for their capacity to hybridize with various plant virus RNAs. PVS clone S12 and PVX clone X6 were found to be very specifie to PVS and PVX RNA respectively, whereas PVY clone Y10 strongly hybridized with PVY RNA and weakly with PVS RNA. Four commercial, nonradioactive Systems of nucleic acid labeling and detection were compared to the usual 32P-labeled probe using dot hybridization experiments. Colorimetric detection of digoxigenin-labeled DNA probes gave a level of sensitivity of 1 ng of virions (60 pg of RNA), similar to autoradiography of 32P-labeled probes. Sulfonated, biotinylated and peroxidase-labeled probes were slightly less sensitive, allowing detection of 600 pg of viral RNA.

Genome subunit reassortment among bunyaviruses analysed by dot hybridization using molecularly cloned complementary DNA probes

Virology ◽  
1984 ◽  
Vol 135 (1) ◽  
pp. 244-256 ◽  
Author(s):  
Craig R. Pringle ◽  
Janice F. Lees ◽  
William Clark ◽  
Richard M. Elliott
Keyword(s):  
Dna Probes ◽  
Complementary Dna ◽  
Dot Hybridization

Application strategies of peptide nucleic acids toward electrochemical nucleic acid sensors

The Analyst ◽  
2021 ◽  
Author(s):  
Qingteng Lai ◽  
Wei Chen ◽  
Yanke Zhang ◽  
Zheng-Chun Liu
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Peptide Nucleic Acids ◽  
Dna Probes ◽  
Highly Sensitive ◽  
Increased Sensitivity ◽  
Acid Sensors

Peptide nucleic acids (PNAs) have attracted tremendous interest in the fabrication of highly sensitive electrochemical nucleic acid biosensor due to their higher stability and increased sensitivity than common DNA probes....

Use of [32P]RNA probes for the dot-hybridization detection of potato spindle tuber viroid

1986 ◽  
Vol 14 (3-4) ◽  
pp. 309-319 ◽  
Author(s):  
D.K. Lakshman ◽  
C. Hiruki ◽  
X.N. Wu ◽  
W.C. Leung
Keyword(s):  
Potato Spindle Tuber Viroid ◽  
Rna Probes ◽  
Dot Hybridization ◽  
Hybridization Detection

scholarly journals Improved In Situ Hybridization Efficiency with Locked-Nucleic-Acid-Incorporated DNA Probes

2006 ◽  
Vol 72 (8) ◽  
pp. 5311-5317 ◽  
Author(s):  
Kengo Kubota ◽  
Akiyoshi Ohashi ◽  
Hiroyuki Imachi ◽  
Hideki Harada
Keyword(s):  
In Situ Hybridization ◽  
Nucleic Acid ◽  
Fluorescence Intensity ◽  
Dna Probes ◽  
Locked Nucleic Acid ◽  
Factors Affecting ◽  
Hybridization Efficiency ◽  
Probe Hybridization ◽  
Major Factors

ABSTRACT Low signal intensity due to poor probe hybridization efficiency is one of the major drawbacks of rRNA-targeted in situ hybridization. There are two major factors affecting the hybridization efficiency: probe accessibility and affinity to the targeted rRNA molecules. In this study, we demonstrate remarkable improvement in in situ hybridization efficiency by applying locked-nucleic-acid (LNA)-incorporated oligodeoxynucleotide probes (LNA/DNA probes) without compromising specificity. Fluorescently labeled LNA/DNA probes with two to four LNA substitutions exhibited strong fluorescence intensities equal to or greater than that of probe Eub338, although these probes did not show bright signals when they were synthesized as DNA probes; for example, the fluorescence intensity of probe Eco468 increased by 22-fold after three LNA bases were substituted for DNA bases. Dissociation profiles of the probes revealed that the dissociation temperature was directly related to the number of LNA substitutions and the fluorescence intensity. These results suggest that the introduction of LNA residues in DNA probes will be a useful approach for effectively enhancing probe hybridization efficiency.

Exocortis virus: An infectious free-nucleic acid plant virus with unusual properties

Virology ◽  
1972 ◽  
Vol 47 (2) ◽  
pp. 456-466 ◽  
Author(s):  
J.S. Semancik ◽  
L.G. Weathers
Keyword(s):  
Nucleic Acid ◽  
Plant Virus ◽  
Acid Plant

Nucleic acid hybridization in plant virus diagnosis and characterization

1988 ◽  
Vol 6 (9) ◽  
pp. 213-218 ◽  
Author(s):  
R HULL
Keyword(s):  
Nucleic Acid ◽  
Plant Virus ◽  
Nucleic Acid Hybridization ◽  
Virus Diagnosis

Colorimetric detection of nucleic acid sequences in plant pathogens based on CRISPR/Cas9 triggered signal amplification

2019 ◽  
Vol 186 (4) ◽  
Author(s):  
Weidan Chang ◽  
Weipeng Liu ◽  
Ying Liu ◽  
Fangfang Zhan ◽  
Huifang Chen ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Signal Amplification ◽  
Plant Pathogens ◽  
Colorimetric Detection ◽  
Nucleic Acid Sequences
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