scholarly journals Analysis of the relief of MCF-7 tumor cells during apoptisis on the basis of phase-contrast images obtained by laser interference microscopy

2021 ◽  
Vol 14 (2) ◽  
pp. 171-176
Author(s):  
A.M. Ignatova ◽  
A.S. Nikityuk ◽  
Y.V. Bayandin ◽  
V.V. Grishko ◽  
O.B. Naimark
Keyword(s):  
Tumor Cells ◽  
Phase Contrast ◽  
Interference Microscopy ◽  
Laser Interference ◽  
Laser Interference Microscopy ◽  
Mcf 7

scholarly journals Label-Free Single Cell Viability Assay Using Laser Interference Microscopy

Biology ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 590
Author(s):  
Yulia Beloglazova ◽  
Aleksandr Nikitiuk ◽  
Anna Voronina ◽  
Olga Gagarskikh ◽  
Yuriy Bayandin ◽  
...  
Keyword(s):  
Power Spectrum ◽  
Cell Viability ◽  
Single Cell ◽  
Interference Microscopy ◽  
Label Free ◽  
Laser Interference ◽  
Laser Interference Microscopy ◽  
Phase Thickness ◽  
Spectrum Slope ◽  
Mcf 7

Laser interference microscopy (LIM) is a promising label-free method for single-cell research applicable to cell viability assessment in the studies of mammalian cells. This paper describes the development of a sensitive and reproducible method for assessing cell viability using LIM. The method, based on associated signal processing techniques, has been developed as a result of real-time investigation in phase thickness fluctuations of viable and non-viable MCF-7 cells, reflecting the presence and absence of their metabolic activity. As evinced by the values of the variable vc, this variable determines the viability of a cell only in the attached state (vc exceeds 20 nm2 for viable attached cells). The critical value of the power spectrum slope βc of the phase thickness fluctuations equals 1.00 for attached MCF-7 cells and 0.71 for suspended cells. The slope of the phase fluctuations’ power spectrum for MCF-7 cells was determined to exceed the threshold value of βc for a living cell, otherwise the cell is dead. The results evince the power spectrum slope as the most appropriate indicator of cell viability, while the integrated evaluation criterion (vc and βc values) can be used to assay the viability of attached cells.

EXAMINATION OF L-TRANSFORMATION IN Salmonella BY ELECTRON AND LASER INTERFERENCE MICROSCOPY

2013 ◽  
pp. 55-60
Author(s):  
A.Yu. Arsenyuk ◽  
◽  
I.B. Pavlova ◽  
P.S. Ignat'ev ◽  
◽  
...  
Keyword(s):  
Interference Microscopy ◽  
Laser Interference ◽  
Laser Interference Microscopy

Phase-modulation laser interference microscopy: an advance in cell imaging and dynamics study

2008 ◽  
Vol 13 (3) ◽  
pp. 034004 ◽  
Author(s):  
Alexey R. Brazhe ◽  
Nadezda A. Brazhe ◽  
Georgy V. Maksimov ◽  
Pavel S. Ignatyev ◽  
Andrey B. Rubin ◽  
...  
Keyword(s):  
Phase Modulation ◽  
Cell Imaging ◽  
Interference Microscopy ◽  
Laser Interference ◽  
Laser Interference Microscopy

Real time laser interference microscopy for bar-spread polystyrene/poly(methyl methacrylate) blends

2014 ◽  
Vol 52 (15) ◽  
pp. 985-992 ◽  
Author(s):  
Ehtsham Ul Haq ◽  
Daniel T. W. Toolan ◽  
Jillian A. Emerson ◽  
Thomas H. Epps ◽  
Jonathan R. Howse ◽  
...  
Keyword(s):  
Methyl Methacrylate ◽  
Real Time ◽  
Interference Microscopy ◽  
Laser Interference ◽  
Poly Methyl Methacrylate ◽  
Laser Interference Microscopy

Diagnostics possibilities of erythrocytes analysis by the method of laser interference microscopy

2021 ◽  
Vol 66 (1) ◽  
pp. 22-25
Author(s):  
A. V. Deryugina ◽  
M. N. Ivashchenko ◽  
P. S. Ignatyev ◽  
A. A. Belov ◽  
V. A. Petrov
Keyword(s):  
Functional Activity ◽  
Interference Microscopy ◽  
Qualitative Assessment ◽  
Critical Conditions ◽  
Laser Interference ◽  
Functional Capabilities ◽  
Surface Nanostructure ◽  
Laser Interference Microscopy ◽  
Microscopy Method ◽  
Altered Cell

The morphofunctional state of erythrocytes significantly changes by the onset and progression of various diseases, as well as in critical conditions, injuries, accompanied by severe tissue damage. This leads to a violation of microcirculation and has an effect on hemorheology in general. In connection with this, there is a need for the development of adequate methods for assessing the functional activity of erythrocytes. The aim of the work was to study the possibilities of laser interference microscopy in the analysis of the functional state of erythrocytes. The results allowed us to quantify the state of the phase height and phase diameter of erythrocytes. Analysis of the surface nanostructure gave a qualitative assessment of the functional activity of cells. The following patterns were revealed: the action of adrenaline causes the appearance of echinocytes with an increase in phase height and decrease phase diameter and the appearance of spicules on the surface of cells. The action of cortisol leads to a decrease in the phase height and the phase diameter with the appearance of numerous loosening of the structure of the cell surface. Moreover, the traditional method of analyzing the distribution of erythrocytes in a population by morphology (discocytes, stomatocytes, and degeneratively altered cell forms) did not reveal statistically significant differences. Thus, the results indicate that the interference microscopy method allows a qualitative assessment of the morphofunctional activity of native cells. This increases the information content of the analysis and leads to the objectification of data on the functional capabilities of erythrocytes.

Use of laser interference microscopy to study the state of UV(B)-irradiated mouse peritoneal macrophages

2012 ◽  
Vol 55 (6) ◽  
pp. 726-730 ◽  
Author(s):  
M. V. Zagubizhenko ◽  
A. I. Yusipovich ◽  
S. K. Pirutin ◽  
V. L. Minaev ◽  
Yu. B. Kudryashov
Keyword(s):  
Peritoneal Macrophages ◽  
The State ◽  
Interference Microscopy ◽  
Laser Interference ◽  
Laser Interference Microscopy ◽  
Mouse Peritoneal Macrophages

Assessing the Functional State of Red Blood Cells by Using the Laser Interference Microscopy

2021 ◽  
Vol 15 (4) ◽  
pp. 388-392
Author(s):  
A. V. Deryugina ◽  
M. N. Ivashchenko ◽  
A. A. Belov ◽  
P. S. Ignatiev ◽  
V. B. Metelin
Keyword(s):  
Red Blood Cells ◽  
Functional State ◽  
Blood Cells ◽  
Interference Microscopy ◽  
Laser Interference ◽  
Laser Interference Microscopy

VITAL MORPHOMETY OF RBC IN THEIR INTERACTION WITH STRESS HORMONES

2020 ◽  
pp. 146-147
Author(s):  
A. Deryugina ◽  
P. Ignatiev ◽  
V. Metelin ◽  
A. Belov ◽  
V. Petrov
Keyword(s):  
Normal State ◽  
Physiological State ◽  
Stress Hormones ◽  
Interference Microscopy ◽  
Extreme Condition ◽  
Laser Interference ◽  
Laser Interference Microscopy ◽  
As Stress ◽  
To Receive

The possibility to use the laser interference microscopy in order to assess the RBCs morphology in normal state and in interaction with cortisol and adrenalin as stress hormones was studied. It was established that the laser interference microscopy represented an important instrument permitting to receive the information about physiological state of the RBCs as well as of the organism in the whole in extreme condition. New aspects of cortisol and adrenalin impact on the RBC morphology are defined

Laser interference microscopy in erythrocyte study

2009 ◽  
Vol 105 (10) ◽  
pp. 102037 ◽  
Author(s):  
A. I. Yusipovich ◽  
E. Yu. Parshina ◽  
N. Yu. Brysgalova ◽  
A. R. Brazhe ◽  
N. A. Brazhe ◽  
...  
Keyword(s):  
Interference Microscopy ◽  
Laser Interference ◽  
Laser Interference Microscopy

Study of cancer and normal cells dynamics based on laser interference microscopy

2020 ◽  
Author(s):  
Aleksander S. Nikitiuk ◽  
Anna O. Voronina ◽  
Yuliya A. Beloglazova ◽  
Olga S. Ozernykh ◽  
Victoria V. Grishko ◽  
...  
Keyword(s):  
Interference Microscopy ◽  
Laser Interference ◽  
Normal Cells ◽  
Laser Interference Microscopy
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