Peer Review #1 of "Skin and fur bacterial diversity and community structure on American southwestern bats: effects of habitat, geography and bat traits (v0.1)"

Field and laboratory investigations were conducted to characterize bacterial diversity and community structure in a badly contaminated mangrove wetland adjacent to the metropolitan area of a megacity in subtropical China. Next-generation sequencing technique was used for sequencing the V4–V5 region of the 16s rRNA gene on the Illumina system. Collectively, Proteobacteria, Chloroflexi, Planctomycetes, Actinobacteria and Bacteroidetes were the predominant phyla identified in the investigated soils. A significant spatial variation in bacterial diversity and community structure was observed for the investigated mangrove soils. Heavy metal pollution played a key role in reducing the bacterial diversity. The spatial variation in soil-borne heavy metals shaped the spatial variation in bacterial diversity and community structure in the study area. Other environmental factors such as total carbon and total nitrogen in the soils that are affected by seasonal change in temperature could also influence the bacterial abundance, diversity and community structure though the temporal variation was relatively weaker, as compared to spatial variation. The bacterial diversity index was lower in the investigated site than in the comparable reference site with less contaminated status. The community structure in mangrove soils at the current study site was, to a remarkable extent, different from those in the tropical mangrove wetlands around the world.

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Interaction between typical sulfonamides and bacterial diversity in drinking water

Journal of Water and Health ◽

10.2166/wh.2018.210 ◽

2018 ◽

Vol 16 (6) ◽

pp. 914-920 ◽

Cited By ~ 2

Author(s):

Qing Wu ◽

Shuqun Li ◽

Xiaofei Zhao ◽

Xinhua Zhao

Keyword(s):

Drinking Water ◽

Community Structure ◽

Microbial Community ◽

Bacterial Community ◽

Bacterial Diversity ◽

Microbial Community Structure ◽

Antibiotic Use ◽

Community Diversity ◽

Wide Range ◽

Growth Of Bacteria

Abstract The abuse of antibiotics is becoming more serious as antibiotic use has increased. The sulfa antibiotics, sulfamerazine (SM1) and sulfamethoxazole (SMZ), are frequently detected in a wide range of environments. The interaction between SM1/SMZ and bacterial diversity in drinking water was investigated in this study. The results showed that after treatment with SM1 or SMZ at four different concentrations, the microbial community structure of the drinking water changed statistically significantly compared to the blank sample. At the genus level, the proportions of the different bacteria in drinking water may affect the degradation of the SM1/SMZ. The growth of bacteria in drinking water can be inhibited after the addition of SM1/SMZ, and bacterial community diversity in drinking water declined in this study. Furthermore, the resistance gene sul2 was induced by SM1 in the drinking water.

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Peer Review #2 of "Comparison of bacterial diversity and abundance between sexes of Leptocybe invasa Fisher & La Salle (Hymenoptera: Eulophidae) from China (v0.1)"

10.7287/peerj.8411v0.1/reviews/2 ◽

2020 ◽

Author(s):

G Dittrich-Schroder

Keyword(s):

Peer Review ◽

Bacterial Diversity ◽

Leptocybe Invasa

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Agricultural practice negatively affects soil microbial diversity and nitrogen functional genes comparing to adjacent native forest soils

10.22541/au.163705519.94774515/v2 ◽

2021 ◽

Author(s):

Tiehang Wu ◽

Michael Sabula ◽

Holli Milner ◽

Gary Strickland ◽

Gan Liu

Keyword(s):

Community Structure ◽

Bacterial Diversity ◽

Forest Soils ◽

Agricultural Practices ◽

Functional Genes ◽

Agricultural Practice ◽

Soil Bacterial Diversity ◽

Soil Microbial Diversity ◽

Soil Microbial ◽

Soil Bacterial

Soil microbial diversity and community are determined by anthropogenic activities and environmental conditions, which greatly affect the functioning of ecosystem. We investigated the soil bacterial diversity, communities, and nitrogen (N) functional genes with different disturbance intensity levels from crop, transition, to forest soils at three locations in the coastal region of Georgia, USA. Illumina high-throughput DNA sequencing based on bacterial 16S rRNA genes were performed for bacterial diversity and community analyses. Nitrifying (AOB amoA) and denitrifying (nirK) functional genes were further detected using quantitative PCR (qPCR) and Denaturing Gradient Gel Electrophoresis (DGGE). Soil bacterial community structure determined by Illumina sequences were significantly different between crop and forest soils (p < 0.01), as well as between crop and transition soils (p = 0.01). However, there is no difference between transition and forest soils. Compared to less disturbed forest, agricultural practice significantly decreased soil bacterial richness and Shannon diversity. Soil pH and nitrate contents together contributed highest for the observed different bacterial communities (Correlations = 0.381). Two OTUs (OTU5, OTU8) belonging to Acidobacteriales species decreased in crop soils, however, agricultural practices significantly increased an OTU (OTU4) of Nitrobacteraceae. The relative abundance of AOB amoA gene was significantly higher in crop soils than in forest and transition soils. Distinct grouping of soil denitrifying bacterial nirK communities was observed and agricultural practices significantly decreased the diversity of nirK gene compared to forest soils. Anthropogenic effects through agricultural practices negatively affecting the soil bacterial diversity, community structure, and N functional genes.

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