scholarly journals Effect of antioxidant and optimal antimicrobial mixtures of carvacrol, grape seed extract and chitosan on different spoilage microorganisms and their application as coatings on different food matrices

2013 ◽  
Vol 2 (1) ◽  
Author(s):  
Javiera F. Rubilar ◽  
Rui M.S. Cruz ◽  
Igor Khmelinskii ◽  
Margarida Cortez Vieira
Keyword(s):  
Synergistic Effects ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Butylated Hydroxytoluene ◽  
Yeast Saccharomyces Cerevisiae ◽  
Dpph Method ◽  
Food Matrices

There is growing interest in the use of natural agents with antimicrobial (AM) and antioxidant (AOX) properties. Optimization of the AM capacity for mixtures containing carvacrol, grape seed extract (GSE) and chitosan, against gram-negative (Pseudomonas aeruginosa), gram-positive bacteria (Staphylococcus aureus, Listeria innocua and Enterococcus faecalis) and yeast (Saccharomyces cerevisiae) at 106 cfu mL-1 was studied. To observe the synergistic or antagonistic effect and find optimal combinations between the three agents, a simplex centroid mixture design was run for each microorganism, combining carvacrol (0-300 ppm, X1), GSE (0-2000 ppm, X2) and chitosan (0-2% w/v, X3). Results of the response surface analysis showed several synergistic effects for all microorganisms. Combinations of 60 ppm-400 ppm-1.2% w/v (carvacrol-GSE-chitosan; optimal AM combination 1, OAMC-1); 9.6 ppm-684 ppm-1.25% w/v (OAMC-2); 90 ppm-160 ppm-1.24% w/v (OAMC-3) were found to be the optimal mixtures for all microorganisms. Radical scavenging activity (RSA) of the same agents was then compared with a standard AOX (butylated hydroxytoluene; BHT) at different concentrations (25, 50 and 100 ppm; as well as the optimal AM concentrations) by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. RSA increased in the following order: chitosan< carvacrol< BHT< GSE and for the OAMC: OAMC-2< OAMC-1< OAMC-3. The best RSA (OAMC-3) was applied as a coating in two different food matrices (strawberries and salmon). For strawberries, P. aeruginosa was more sensitive to the action of OAMC-3 than S. cerevisiae. For salmon, S. aureus was more resistant to the action of OAMC-3 than E. faecalis and L. innocua.

scholarly journals Effect of antioxidant and optimal antimicrobial mixtures of carvacrol, grape seed extract and chitosan on different spoilage microorganisms and their application as coatings on different food matrices

2013 ◽  
Vol 2 (1) ◽  
Author(s):  
Javiera F. Rubilar ◽  
Rui M.S. Cruz ◽  
Igor Khmelinskii ◽  
Margarida Cortez Vieira
Keyword(s):  
Synergistic Effects ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Butylated Hydroxytoluene ◽  
Yeast Saccharomyces Cerevisiae ◽  
Dpph Method ◽  
Food Matrices

There is growing interest in the use of natural agents with antimicrobial (AM) and antioxidant (AOX) properties. Optimization of the AM capacity for mixtures containing carvacrol, grape seed extract (GSE) and chitosan, against gram-negative (Pseudomonas aeruginosa), gram-positive bacteria (Staphylococcus aureus, Listeria innocua and Enterococcus faecalis) and yeast (Saccharomyces cerevisiae) at 106 cfu mL-1 was studied. To observe the synergistic or antagonistic effect and find optimal combinations between the three agents, a simplex centroid mixture design was run for each microorganism, combining carvacrol (0-300 ppm, X1), GSE (0-2000 ppm, X2) and chitosan (0-2% w/v, X3). Results of the response surface analysis showed several synergistic effects for all microorganisms. Combinations of 60 ppm-400 ppm-1.2% w/v (carvacrol-GSE-chitosan; optimal AM combination 1, OAMC-1); 9.6 ppm-684 ppm-1.25% w/v (OAMC-2); 90 ppm-160 ppm-1.24% w/v (OAMC-3) were found to be the optimal mixtures for all microorganisms. Radical scavenging activity (RSA) of the same agents was then compared with a standard AOX (butylated hydroxytoluene; BHT) at different concentrations (25, 50 and 100 ppm; as well as the optimal AM concentrations) by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. RSA increased in the following order: chitosan< carvacrol< BHT< GSE and for the OAMC: OAMC-2< OAMC-1< OAMC-3. The best RSA (OAMC-3) was applied as a coating in two different food matrices (strawberries and salmon). For strawberries, P. aeruginosa was more sensitive to the action of OAMC-3 than S. cerevisiae. For salmon, S. aureus was more resistant to the action of OAMC-3 than E. faecalis and L. innocua.

scholarly journals Preparation and Characterisation of Polyphenol-HP-β-Cyclodextrin Inclusion Complex that Protects Lamb Tripe Protein against Oxidation

Molecules ◽  
2019 ◽  
Vol 24 (24) ◽  
pp. 4487 ◽  
Author(s):  
Wenhui Li ◽  
Lidan Ran ◽  
Fei Liu ◽  
Ran Hou ◽  
Wei Zhao ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Inclusion Complex ◽  
Inclusion Complexes ◽  
Radical Scavenging ◽  
Three Dimensional ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Myofibrillar Protein ◽  
Seed Extract ◽  
Target Protein

Grape seed extract (GSE) displays strong antioxidant activity, but its instability creates barriers to its applications. Herein, three HP-β-CD/GSE inclusion complexes with host–guest ratios of 1:0.5, 1:1, and 1:2 were successfully prepared by co-precipitation method to improve stability. Successful embedding of GSE in the HP-β-CD cavity was confirmed by fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), differential scanning calorimetry (DSC), and scanning electron microscopy (SEM) analyses. The Autodock Tools 1.5.6 was used to simulate the three-dimensional supramolecular structure of the inclusion complex of 2-hydroxypropyl-β-cyclodextrin and grape seed extract (HP-β-CD/GSE) by molecular docking. The MALDI-TOF-MS technology and chemical database Pubchem, and structural database PDB were combined to reconstitute the three-dimensional structure of target protein. The binding mode of the HP-β-CD/GSE inclusion complex to target protein was studied at the molecular level, and the antioxidant ability of the resulting HP-β-CD/GSE inclusion complexes was investigated by measuring 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging. The effects of HP-β-CD/GSE on myofibrillar protein from lamb tripe were also investigated under oxidative conditions. The positions and interactions of the binding sites of HP-β-CD/GSE inclusion complexes and target protein receptors were simulated by molecular docking. The results showed that HP-β-CD/GSE inclusion complexes were successfully prepared, optimally at a molar ratio of 1:2. At low (5 μmol/g) to medium (105 μmol/g) concentrations, HP-β-CD/GSE inclusion complexes decreased the carbonyl content, hydrophobicity, and protein aggregation of myofibrillar protein from lamb tripe, and increased the sulphydryl content. Furthermore, high concentration (155 μmol/g) of HP-β-CD/GSE inclusion complexes promoted protein oxidation.

scholarly journals Synergistic Effects of Exogenous Grape Seed Extract and α-Tocopherol on Lipid Oxidation in Refrigerated and Frozen Rainbow Trout (Oncorhynchus Mykiss) and Pork Loin (Longissimus Dorsi) Muscle

2021 ◽  
Vol 7 (2) ◽  
pp. 1-6
Author(s):  
Robert G Brannan ◽  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Lipid Oxidation ◽  
Synergistic Effects ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Longissimus Dorsi ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Pork Loin

The objective of this study was to assess the antioxidative effectiveness of Grape Seed Extract (GSE) with and without exogenous a-tocopherol in two raw meats that are particularly susceptible to lipid oxidation, rainbow trout (Oncorhynchus mykiss) and pork loin (longissimus dorsi).

Grape Seeds Extract as Brain Food: A Review

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Souad El Gengaihi ◽  
Doha H. Abou Baker
Keyword(s):  
Antioxidant Activities ◽  
Complex Mixture ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Amyloid Peptide ◽  
Grape Seeds ◽  
Potential Benefits ◽  
Reduced Risk

Interest in the biological role of bioactive compounds present in medicinal herbs has increased over the last years. Of particular interest are plants that have an anti-Alzheimer activities. Several plants can be useful for Alzheimer (AD) management. Such as these which have anti-inflammatory activity, acetylcholinesterase (AChE) inhibitory action, antiapoptotic, slow the aggregation of amyloid peptide and antioxidant activities. Grape seed extract (GSE) is a complex mixture of several compounds, mostly represented by polyphenols and flavonoids. Their consumption is safe and is recognized to exert several health benefits. GS flavonoids have been associated with the reduced risk of chronic diseases, we present some findings on the potential benefits of GSE for the treatment of AD.

Grape Seed Extract Complex (FFM-GS) Inhibits Postmenopausal Symptoms: In Vitro and In Vivo S

2019 ◽  
Vol 48 (3) ◽  
pp. 297-305
Author(s):  
Minhee Lee ◽  
Da-Eun Nam ◽  
Soo-Jeung Park ◽  
Dakyung Kim ◽  
Jeong-Moon Yun ◽  
...  
Keyword(s):  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Postmenopausal Symptoms

scholarly journals Comparative Evaluation of Immediate Bond Strength to Bleached Enamel Following Application of Various Antioxidant Solutions

2017 ◽  
Vol 05 (02) ◽  
pp. 084-089
Author(s):  
Alisha Dhingra ◽  
Ashu Gupta ◽  
Anshu Minocha ◽  
Nayantara Sen
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
The Other ◽  
Sodium Ascorbate ◽  
Group V ◽  
Group I ◽  
Significant Difference

Abstract Aim: The aim of this study was to compare the reversal of shear bond strength of composite to bleached enamel immediately after bleaching followed by application of various antioxidant solutions. Material and Methods: Seventy central incisors were divided into seven groups. Groups I and II served as unbleached and bleached controls respectively. Groups III, IV, V, VI and VII served as the experimental groups and were subjected to 37.5% hydrogen peroxide bleaching followed by 10 min application of 10% sodium ascorbate, 25% alpha-tocopherol, 6.5% grape seed extract, 5% lycopene and 5% green tea extract respectively. Following composite bonding, shear bond strength was determined and the results were analyzed using ANOVA followed by Post Hoc Multiple Comparisons test. Results: The bond strength values for Group I (positive control) were maximum and significantly different than all the other groups except Grape seed extract group (Group V). When compared to Group II (bleached control), all the groups showed significantly higher bond strength. Significant difference in the bond strength values were seen between Group III (10% sodium ascorbate) and Group V. Also values for Group V were significantly different from Group VI (5% lycopene). All the other values showed insignificantly different results. Conclusion: All the antioxidant solutions improved the shear bond strength values after bleaching but only Grape seed extract application reversed the values to the non bleached levels. Lycopene was least effective. Other antioxidants showed comparable results.

Incorporation of grape seed extract towards wound care product development

3 Biotech ◽  
2021 ◽  
Vol 11 (6) ◽  
Author(s):  
Amita Ajit ◽  
A. G. Vishnu ◽  
Prashanth Varkey
Keyword(s):  
Product Development ◽  
Wound Care ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Care Product
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