scholarly journals Chemical constituents in leaves and aroma products of Nicotiana rustica L. tobacco

Author(s):  
Venelina T. Popova ◽  
Tanya A. Ivanova ◽  
Albena S. Stoyanova ◽  
Violeta V. Nikolova ◽  
Margarita H. Docheva ◽  
...  

Nicotiana rustica L. (Aztec tobacco) is the only Nicotiana species, except common tobacco (N. tabacum L.), which is cultivated for tabacco products. The leaves of N. rustica, however, accumulate various specialized metabolites of potential interest. Therefore, the objective of this study was to evaluate certain classes of metabolites (by HPLC and GC-MS) in the leaves, the essential oil (EO), concrete and resinoid of N. rustica. Three pentacyclic triterpenes were identified in the leaves (by HPLC): betulin (252.78 µg g-1), betulinic (182.53 µg g-1) and oleanolic  (69.44 µg g-1) acids. The dominant free phenolic acids in the leaves (by HPLC) were rosmarinic (4257.38 µg g-1) and chlorogenic (1714.40 µg g-1), and conjugated forms of vanillic (3445.71 µg g-1), sinapic (1963.11 µg g-1), and syringic (1784.96 µg g-1). The major flavonoids in the leaves were luteolin (960.44 µg g-1), apigenin (880.66 µg g-1) and hyperosid (780.72 µg g-1). The GS-MS profiling of the EO identified 19 components and the major ones were phytol (43.68 %), solanone (5.54 %), cis-5-butyl-4-methyldihydrofuran-2(3H)-one (5.23 %), dihydro-β-ionone (4.25 %), α-ionene (3.54 %),and β-damascenone (3.03 %). The major volatiles in the concrete were  isoamyl alcohol (28.82 %), oxynicotine (9.02 %), phytol (7.80 %), 4-mеthyl-1-penthanol (6.33 %), cotinine (5.55 %) and 3-metyl-3-penthanol (4.09 %). Resinoid composition was dominant by nicotine (39.75 %), phytol (11.23 %), eicosane (4.88 %), diethyl phthalate (4.19 %), dibutyl phthalate (3.48 %) and solanone (3.27 %). Concrete and resinoid showed weak antibacterial activity . These results create grounds for considering N. rustica as a source to obtain aroma or other bioproducts.

2009 ◽  
Vol 4 (7) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Flor D. Mora ◽  
María Araque ◽  
Luis B. Rojas ◽  
Rosslyn Ramírez ◽  
Bladimiro Silva ◽  
...  

Chemical constituents of the essential oil from the leaves of Minthostachys mollis (Kunth) Griseb Vaught var. mollis collected in January 2008 at Tuñame, Trujillo State, Venezuela, were separated and identified by GCMS analysis. The essential oil was obtained by hydrodistillation and thirteen components (98.5% of the sample) were identified by comparison with the Wiley GCMS library data base. The two major components were pulegone (55.2%) and trans-menthone (31.5%). The essential oil showed a significant inhibitory effect against Gram-positive and Gram-negative bacteria, especially Bacillus subtilis and Salmonella typhi (4 μg/mL).


2019 ◽  
Vol 23 (1) ◽  
pp. 57-60
Author(s):  
Susan Joshi ◽  
Sundar Bashyal

The essential oil from the air dried powder of wild Acorus calamus L. rhizomes was isolated using Clevenger-type hydrodistillation apparatus and its chemical constituents were analyzed by gas chromatography-mass spectrometric (GC-MS) analysis. A total numbers of fourteen chemical constituents were identified and quantified occupying 100 % of total oil composition. The major chemical constituents are reported to be β-asarone (68.96 %), 4-hydroxy-4-methyl-2-pentanone (8.73 %) shyobunone (8.65 %), p-methoxyphenyl butanone (4.16 %) and the minor chemical constituents are δ-cadinene (0.84 %), isoelemicine (2.68 %), muurolene (1.92 %) and cis-ocimene (1.44 %). The antibacterial activity of the essential oil isolated from the wild Acorus calamus L. rhizomes was carried out using paper disc method against one Gram positive bacteria of Staphylococcus aureus and three Gram negative bacteria of Escherichia coli, Klebsiella pneumoniae and Proteus vulgaris. The isolated essential oil from the dry powder of Acorus calamus L. rhizome showed a good potentiality of the antibacterial effect against tested bacteria.


2008 ◽  
Vol 3 (6) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Teresa Díaz ◽  
Flor D. Mora ◽  
Judith Velasco ◽  
Tulia Díaz ◽  
Luis B. Rojas ◽  
...  

The chemical constituents of the essential oil obtained by hydrodistillation from the leaves of Calycolpus moritzianus (O. Berg) Burret, syn Psidium caudatum Mc Vaught, collected in November 2006 in Mérida State, Venezuela, were identified by GC-MS analysis. Thirty components (91.1% of the sample) were identified, of which the seven major ones were β-caryophyllene (21.9%), α-pinene (10.9%), viridiflorol (9.7%), β-selinene (6.1%), α-copaene (6.3%), α-selinene (5.3%) and γ-eudesmol (5.1%). The oil was found to have antibacterial activity against Staphylococcus aureus ATCC (6538) and Enterococcus faecalis ATCC (29212), with MIC values of 60 μg/mL and 180 μg/mL, respectively.


2010 ◽  
Vol 50 (S1) ◽  
pp. 70-73 ◽  
Author(s):  
A. Senthil Kumar ◽  
V. Venkatesalu ◽  
K. Kannathasan ◽  
M. Chandrasekaran

2021 ◽  
Vol 1025 ◽  
pp. 242-246
Author(s):  
Nor Naimah Hussin ◽  
Noor Suhana Adzahar ◽  
Thong Chuan Lee ◽  
Jayarama Reddy Venugopal

The chemical constituents of Psidium guajava essential oil was analyzed by using Gas Chromatography-Mass Spectrometry. The essential oil was obtained from dried leaves by hydrodistillation method using a Clevenger apparatus. From the initial screening, 20 components were detected with different amounts traced. The most abundant constituents observed in the oil were Limonene (23.39%), Caryophyllene (18.19%), Cyclooctasiloxane (14.46%), and Pinene (13.50%). The essential oil showed significant antibacterial activity against Staphylococcus aureus, Bacillus subtilis, Enterococcus faecalis and Bacillus cereus with inhibition range of 6-25 mm. The profile of chemical constituents present in the oil able to serve as preliminary screening of the bioactive components posses in a natural plants for investigating antibacterial properties in regenerative medicine.


2020 ◽  
Vol XI (22) ◽  
pp. 22-29
Author(s):  
Hong Thien VAN ◽  
Vo Thu Hien TRAN ◽  
Nu Han Ni TON ◽  
Thao Nguyen LUU ◽  
Nguyen Tuong An HUYNH ◽  
...  

2011 ◽  
Vol 6 (10) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Bahri Fouad ◽  
Romane Abderrahmane ◽  
Arjouni Youssef ◽  
Harrak Rajae ◽  
M. Ahmed El Alaoui El Fels

The antibacterial activity of the essential oil from leaves of Juniperus phoenicea (Cupressaceae) and its chemical constituents were investigated in this study. The essential oil was obtained by hydrodistillation and analyzed by gas chromatography equipped with a flame ionisation detector (GC-FID), and gas chromatography coupled to a mass spectrometer (GC-MS). Sixty-three volatile compounds were identified representing 52 to 92% of the total oil compositions. The main monoterpenes were α-pinene (26.7-78.7%) and δ-3carene (7.6-15.4%). The antibacterial activity of J. phoenicea essential oil, when tested against both Gram-positive and Gram-negative bacteria, showed high activity against all bacteria tested, except Pseudomonas.


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