Effects of Triacontanol on Pepper Plants Infected with Tomato spotted wilt virus (TSWV)

2021 ◽  
Author(s):  
Irina Moskova ◽  
Iskren Sergiev ◽  
Bistra Dikova
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Tomato Spotted Wilt ◽  
Wilt Virus ◽  
Pepper Plants

scholarly journals Overwintering Viruliferous Frankliniella occidentalis (Thysanoptera: Thripidae) as an Infection Source of Tomato spotted wilt virus in Green Pepper Fields

Plant Disease ◽  
2007 ◽  
Vol 91 (7) ◽  
pp. 842-846 ◽  
Author(s):  
Shinichiro Okazaki ◽  
Mitsuru Okuda ◽  
Kazuhiro Komi ◽  
Hideaki Yoshimatsu ◽  
Toru Iwanami
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Frankliniella Occidentalis ◽  
Activity Level ◽  
Weed Species ◽  
Green Pepper ◽  
Source Of Infection ◽  
Tomato Spotted Wilt ◽  
Infection Source ◽  
Wilt Virus ◽  
Pepper Plants

Populations of overwintering viruliferous Frankliniella occidentalis were evaluated in Tomato spotted wilt virus (TSWV)-affected green pepper fields in Bungo-Ohno City, Oita Prefecture, Japan. A survey of TSWV-infected weeds showed that the incidence of infection was low in weeds. Stellaria aquatica was infected frequently; however, the infections were considered secondary cases since S. aquatica appeared in the fields around late February to early March. In contrast, TSWV was frequently detected from green pepper fruits until they rotted. F. occidentalis primarily inhabited and reproduced on the green pepper fruits and moved to Lamium amplexicaule when the fruits rotted and subsequently spread to other weed species as young shoots or flowers appeared. The flying activity level of F. occidentalis rose in late February, and viruliferous F. occidentalis transmitted TSWV to green pepper plants. We concluded that TSWV-infected green pepper fruits discarded in greenhouses and fields are the major source of infection.

scholarly journals First Report of Tomato spotted wilt virus in Peppers and Tomato in the Dominican Republic

Plant Disease ◽  
2014 ◽  
Vol 98 (1) ◽  
pp. 163-163 ◽  
Author(s):  
R. T. Martínez ◽  
S. Poojari ◽  
S. A. Tolin ◽  
X. Cayetano ◽  
R. A. Naidu
Keyword(s):  
Dominican Republic ◽  
Tomato Spotted Wilt Virus ◽  
Caribbean Region ◽  
Rt Pcr ◽  
Spot Virus ◽  
Tomato Spotted Wilt ◽  
Fruit Samples ◽  
The Caribbean ◽  
Wilt Virus ◽  
Pepper Plants

In the Dominican Republic, green bell pepper (Capsicum annuum L.) and tomato (Solanum lycopersicum L.) are widely cultivated under protected greenhouse conditions as high value commercial crops for export. For the past 2 to 3 years, pepper and tomato have been observed in protected crop facilities in Jarabacoa and Constanza in the North Region with chlorotic and necrotic spots and rings on leaves, petioles, and stems, leaf bronzing, and tip necrosis. Fruits on symptomatic pepper and tomato plants showed concentric rings, irregular chlorotic blotches and deformation, and uneven maturation and development. Incidence on pepper and tomato was 20 to 100% and 5 to 20%, respectively. In initial tests, leaves and fruits from each of 20 symptomatic tomato and pepper plants from several greenhouse facilities were reactive in Tomato spotted wilt virus (TSWV; genus Tospovirus, family Bunyaviridae) immunostrip assays (Agdia, Inc., Elkhart, IN). Since these immunostrips are known to react with other tospoviruses, such as Tomato chlorotic spot virus (TCSV) and Groundnut ring spot virus, additional molecular diagnostic assays were conducted. Leaf and fruit samples from symptomatic plants were imprinted on nitrocellulose membrane (NCM) (2), air-dried, and sent to Washington State University for confirmatory tests. Viral nucleic acids were eluted from NCM discs (1) and subjected to reverse transcription (RT)-PCR using primers gL3637 (CCTTTAACAGTDGAAACAT) and gL4435 (CATDGCRCAAGARTGRTARACAGA) designed to amplify a portion of the L RNA segment of several tospoviruses (3). A single DNA product of ~800 bp was amplified from all samples. Amplicons from two tomato (leaf and fruit) and one pepper fruit samples were cloned separately into pCR2.1 (Invitrogen Corp., Carlsbad, CA). Two independent clones per amplicon were sequenced in both orientations. Sequence analyses of these clones (GenBank Accession Nos. KF 219673 to 75) showed 100% nucleotide sequence identity among themselves and 97% identity with corresponding L RNA sequences of pepper isolates of TSWV from Taiwan (HM180088) and South Korea (HM581940), 94 to 95% with tomato isolates of TSWV from South Korea (HM581934) and Hawaii (AY070218), and 89% with a tomato isolate from Indonesia (FJ177301). These results further confirm the presence of TSWV in symptomatic tomato and pepper plants. A comparison of TSWV sequences from the Dominican Republic with TSWV isolates from the United States and other countries in the Caribbean region could not be made due to the absence of corresponding sequences of the L-RNA of the virus from these countries in GenBank. TSWV-positive samples were negative for TCSV in RT-PCR, indicating the absence of this tospovirus that has been reported in the Caribbean region (data not shown). To our knowledge, this is the first confirmed report of TSWV in tomatoes and peppers in the Dominican Republic. The presence of vector thrips, Frankliniella occidentalis, on symptomatic plants was also confirmed, suggesting a role in the spread of TSWV under greenhouse conditions. Recent surveys identified some greenhouses with 100% symptomatic peppers. The presence of TSWV in tomato and pepper has important implications for the domestic and export vegetable industry in the Dominican Republic because of the broad host range of the virus (4). It is critical for commercial producers to monitor TSWV and deploy appropriate management strategies to limit virus spread. References: (1) O. J. Alabi et al. J. Virol. Methods 154:111, 2008. (2) P.-G. S. Chang et al. J. Virol. Methods 171:345, 2011. (3) F. H. Chu et al. Phytopathology 91:361, 2001. (4) G. Parrella et al. J. Plant Pathol. 85:227, 2003.

scholarly journals Current Status and Potential of RNA Interference for the Management of Tomato Spotted Wilt Virus and Thrips Vectors

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 320
Author(s):  
Alexander Nilon ◽  
Karl Robinson ◽  
Hanu R. Pappu ◽  
Neena Mitter
Keyword(s):  
Rna Interference ◽  
Tomato Spotted Wilt Virus ◽  
Cultural Practices ◽  
Management Strategies ◽  
Current Status ◽  
Horticultural Crops ◽  
Yield And Quality ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

Tomato spotted wilt virus (TSWV) is the type member of the genus Orthotospovirus in the family Tospoviridae and order Bunyavirales. TSWV, transmitted by several species of thrips, causes significant disease losses to agronomic and horticultural crops worldwide, impacting both the yield and quality of the produce. Management strategies include growing virus-resistant cultivars, cultural practices, and managing thrips vectors through pesticide application. However, numerous studies have reported that TSWV isolates can overcome host-plant resistance, while thrips are developing resistance to pesticides that were once effective. RNA interference (RNAi) offers a means of host defence by using double-stranded (ds) RNA to initiate gene silencing against invading viruses. However, adoption of this approach requires production and use of transgenic plants and thus limits the practical application of RNAi against TSWV and other viruses. To fully utilize the potential of RNAi for virus management at the field level, new and novel approaches are needed. In this review, we summarize RNAi and highlight the potential of topical or exogenous application of RNAi triggers for managing TSWV and thrips vectors.

Lipid in a Purified Preparation of Tomato Spotted Wilt Virus

Nature ◽  
1964 ◽  
Vol 203 (4945) ◽  
pp. 671-672 ◽  
Author(s):  
RUPERT J. BEST ◽  
GERARD F. KATEKAR
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

scholarly journals First Report of a Resistance-Breaking Strain of Tomato spotted wilt virus Infecting Tomatoes With the Sw-5 Tospovirus-Resistance Gene in California

Plant Disease ◽  
2017 ◽  
Vol 101 (4) ◽  
pp. 637-637 ◽  
Author(s):  
O. Batuman ◽  
T. A. Turini ◽  
P. V. Oliveira ◽  
M. R. Rojas ◽  
M. Macedo ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Tomato Spotted Wilt Virus ◽  
Breaking Strain ◽  
First Report ◽  
Tomato Spotted Wilt ◽  
Resistance Breaking ◽  
Wilt Virus
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