Associations between Feeding Behaviors Collected from an Automated Milk Feeder and Neonatal Calf Diarrhea in Group Housed Dairy Calves: A Case-Control Study

The objective of this case-control study was to determine if feeding behavior data collected from an automated milk feeder (AMF) could be used to predict neonatal calf diarrhea (NCD) in the days surrounding diagnosis in pre-weaned group housed dairy calves. Data were collected from two research farms in Ontario between 2017 and 2020 where calves fed using an AMF were health scored daily and feeding behavior data (milk intake (mL/d), drinking speed (mL/min), number of rewarded or unrewarded visits) was collected. Calves with NCD were pair matched to healthy controls (31 pairs) by farm, gender, and age at case diagnosis to assess for differences in feeding behavior between case and control calves. Calves were first diagnosed with NCD on day 0, and a NCD case was defined as calves with a fecal score of ≥2 for 2 consecutive days, where control calves remained healthy. Repeated measure mixed linear regression models were used to determine if there were differences between case and control calves in their daily AMF feeding behavior data in the days surrounding diagnosis of NCD (−3 to +5 days). Calves with NCD consumed less milk on day 0, day 1, day 3, day 4 and day 5 following diagnosis compared to control calves. Calves with NCD also had fewer rewarded visits to the AMF on day −1, and day 0 compared to control calves. However, while there was a NCD status x day interaction for unrewarded visits, there was only a tendency for differences between NCD and control calves on day 0. In this study, feeding behaviors were not clinically useful to make diagnosis of NCD due to insufficient diagnostic ability. However, feeding behaviors are a useful screening tool for producers to identify calves requiring further attention.

GLP-1 Suppresses Feeding Behaviors and Modulates Neuronal Electrophysiological Properties in Multiple Brain Regions

The glucagon-like peptide-1 (GLP-1) plays important roles in the regulation of food intake and energy metabolism. Peripheral or central GLP-1 suppresses food intake and reduces body weight. The electrophysiological properties of neurons in the mammalian central nervous system reflect the neuronal excitability and the functional organization of the brain. Recent studies focus on elucidating GLP-1-induced suppression of feeding behaviors and modulation of neuronal electrophysiological properties in several brain regions. Here, we summarize that activation of GLP-1 receptor (GLP-1R) suppresses food intake and induces postsynaptic depolarization of membrane potential and/or presynaptic modulation of glutamatergic or GABAergic neurotransmission in brain nuclei located within the medulla oblongata, pons, mesencephalon, diencephalon, and telencephalon. This review may provide a background to guide future research about the cellular mechanisms of GLP-1-induced feeding inhibition.

An Overview of Appetite-Regulatory Peptides in Addiction Processes; From Bench to Bed Side

There is a substantial need for new pharmacological treatments of addiction, and appetite-regulatory peptides are implied as possible candidates. Appetite regulation is complex and involves anorexigenic hormones such as glucagon-like peptide-1 (GLP-1) and amylin, and orexigenic peptides like ghrelin and all are well-known for their effects on feeding behaviors. This overview will summarize more recent physiological aspects of these peptides, demonstrating that they modulate various aspects of addiction processes. Findings from preclinical, genetic, and experimental clinical studies exploring the association between appetite-regulatory peptides and the acute or chronic effects of addictive drugs will be introduced. Short or long-acting GLP-1 receptor agonists independently attenuate the acute rewarding properties of addictive drugs or reduce the chronic aspects of drugs. Genetic variation of the GLP-1 system is associated with alcohol use disorder. Also, the amylin pathway modulates the acute and chronic behavioral responses to addictive drugs. Ghrelin has been shown to activate reward-related behaviors. Moreover, ghrelin enhances, whereas pharmacological or genetic suppression of the ghrelin receptor attenuates the responses to various addictive drugs. Genetic studies and experimental clinical studies further support the associations between ghrelin and addiction processes. Further studies should explore the mechanisms modulating the ability of appetite-regulatory peptides to reduce addiction, and the effects of combination therapies or different diets on substance use are warranted. In summary, these studies provide evidence that appetite-regulatory peptides modulate reward and addiction processes, and deserve to be investigated as potential treatment target for addiction.

Laser Enrichment Device Stimulates Broiler Laser-Following Behavior While Increasing Individual Bird Locomotion and Pen-Wide Movement

Genetic selection for fast growth rate and high breast muscle yield in modern broilers has unintended effects on animal welfare and behavior, namely in terms of inactivity and leg disorders. We hypothesized that exercise stimulated through environmental enrichment could positively stimulate pen-wide activity and improve bird welfare. The study objectives were to implement a laser enrichment device to motivate active and feeding behaviors throughout the pen. Twelve hundred Ross 708 broilers were randomly assigned to enrichment (LASER; laser enrichment, or CON; no laser enrichment) for 49 d. Seventy focal birds were randomly assigned to 14 video-recorded pens for behavioral analysis, including focal bird home pen behavior and walking distance. Pen-wide activity was also measured during the 4-min laser periods, four times daily, d0–8, and 1 day weekly, wk 1–6. Focal birds were gait scored wk 1–6, and were euthanized on d42 for tibia bone mineral content, density, and bone breaking strength analysis. Time spent active was increased in LASER-enriched birds compared to CON on wk 3–5 by up to 214% (wk 4), and percent of time at the feeder was increased in LASER-enriched birds by 761% on wk 4 (P < 0.05). Peak percent of birds following the laser (LASER-enriched pens only) was observed on d0 (8.52%). Over wk 1–6, peak laser-following behavior was observed on wk 3 (3.07% of birds). Percent of birds moving during laser periods was increased in LASER-enriched pens on d0, 1, 2, 6, 7, and 8, with a percent increase of 68.7% observed on d1 (P < 0.05). Percent of birds moving (laser-following or not) was increased on wk 1, 3, and 4 in LASER-enriched pens, with an increase of 69.7% observed on wk 4 (P < 0.05). No differences were found in tibia measures. These data indicate that laser enrichment stimulated voluntary locomotion through wk 5 and laser-following behavior through wk 6, and that the relatively small percent of birds actively following the laser stimulated pen-wide movement above the level of the CON through wk 4 on study.

Parent-reported feeding characteristics in children with ASD vs. children who are typically developing

Background Parents of children with autism spectrum disorders (ASDs) often report that their children have multiple dietary behavior problems than parents of typically developing (TD) children do. This may affect proper nutrition and subsequently adequate growth and development in children with ASD. The current study aimed to assess the feeding behavior in recently diagnosed children with autism spectrum disorders (ASD) ranged in age from 2 to 4 years and compare it with typically developing (TD) children and to explore the relationship between feeding behaviors and autism severity using the Montreal Children’s Hospital Feeding Scale (MCH) and Childhood Autism Rating Scale (CARS), respectively. Parents of 35 preschool children (2–4 years) with ASD completed reports of physical measurements, feeding interview, Childhood Autism Rating Scale (CARS), and Montreal Children’s Hospital Feeding Scale (MCH Feeding-Scale). The collected data from parents of children with ASD were analyzed and compared with 70 typically developed children matched with age and sex. Results ASD children showed statistically significantly more problematic feeding behaviors in most of the assessed eating characters compared with TD children, e.g., food neophobia, eating non-food items instead of food, requiring assistance during eating, and feeding avoidance to particular textures and taste. The mean total score of the MCH feeding scale differed significantly between children with ASD and TD children. No correlation was found between the mean total score of the MCH feeding scale and CARS scores. Conclusion Our findings revealed a high rate of behavioral feeding problems in children with ASD. Future work will be needed to follow up the feeding behaviors and to develop practical feeding approaches for ASD children to maintain nutritional adequacy.

PSII-15 Investigations of feeding preferences for common palatants in equine weanlings

Work in other species has demonstrated an impact of gestational exposure on flavor preferences. Although equine palatability preferences in mature horses have been identified, feeding behaviors in growing horses are not well documented. The objective of this study was to evaluate the impact of exposure to common palatants in utero on feeding preferences of weanlings. Stock-type weanlings (n = 4) weighing 225(±18.15) kg at 29(±2.55) weeks of age were used in a side-by-side comparison test with a divided feeding platform. Common palatants (apple and anise) were utilized throughout the broodmare gestational period. A novel palatant, peppermint, was withheld during gestation to identify impacts associated with neophobic tendencies. Treatments were offered once daily for three consecutive days across three periods (4 weeks apart) using a pelleted concentrate feed with an application rate of 0.375 mL/0.23 kg. Weanlings were allowed free access to the feeding platform with identical pans spaced 6” apart for 20 minutes. Treatment location within the feeding platform was changed daily to limit effects associated with side-eating. Data were measured as a Chi Square test of SAS using PROC FREQ statement with α ≤. 0.05. Despite gestational exposure to apple and anise, no preference was measured in palatability assessments including First Sniff (P = 0.29), First Consumed (P = 0.70), First Finished (P = 0.29). These data suggest that exposure in utero failed to elicit a preference for common palatants in weanling horses. Additionally, these data fail to demonstrate a neophobic effect for a novel palatant, peppermint.

151 Intake, Growth, and Insectifugal Outcomes Associated with Free-choice Supplementation of Garlic Products to Feedlot Steers

Conflicting anecdotal accounts from producers about their cattle performance and fly-repellence outcomes while receiving garlic-infused supplements have limited feeding decisions. This study evaluated differences in animal performance and insectifugal outcomes among four groups (26 steers per group) over two years (total = 208 steers). The steers either received non-garlic mineral supplement (MS), MS+0.3% garlic oil-based premix (GOil), MS+2.5% garlic powder (LGP), or MS+5% garlic powder (HGP). A completely randomized design was implemented where each steer was assigned to each treatment group while balancing for bodyweight and breed composition. Steers received a feedlot ration and free-choice mineral supplement for 86 and 108 d in the first and second year, respectively. Individual feed and supplement consumption were measured with automatic feeding units. Fly abundance on each animal was determined using digital images collected during weekly fly surveys. The ADG, feed efficiency, ultrasound body composition, and intake-related activities were also evaluated during the study. Data were analyzed using linear- or generalized linear mixed models in SAS. A significant year and Group×year interactions were observed for daily supplement intake (P < 0.02). Similar interactions (P < 0.04) were observed for feeding behaviors associated with supplement intake. Fly abundance during the peak months was below the economic threshold and was not different among the groups (P = 0.25), but the year and Group×year interactions were significant (P < 0.02). There were no differences among the groups for DMI (P = 0.81), ADG (P = 0.54) and gain to feed ratio (P = 0.34). The Group×year interactions were present for the change in ultrasound rib-fat-thickness. The reasons behind the differences in supplement intake are not clear but may be related to the garlic products’ phytochemical profiles. These results suggest that further studies are required to identify the specific bioactive compounds in garlic that affect supplement intake.