scholarly journals A central role for a region in the middle

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Edward Stuttfeld ◽  
Stefan Imseng ◽  
Timm Maier

A domain called the 'Conserved region in the middle' is responsible for target recognition in the TORC2 complex in fission yeast and the mTORC2 complex in mammals.

2019 ◽  
Author(s):  
Alberto Elías-Villalobos ◽  
Damien Toullec ◽  
Céline Faux ◽  
Martial Séveno ◽  
Dominique Helmlinger

AbstractTranscription initiation involves the coordinated activities of large multimeric complexes that are organized into functional modules. Little is known about the mechanisms and pathways that govern their assembly from individual components. We report here several principles governing the assembly of the highly conserved SAGA and NuA4 co-activator complexes. Using fission yeast, which contain two functionally non-redundant paralogs of the shared Tra1 subunit, we demonstrate that Tra1 contributes to scaffolding the entire NuA4 complex. In contrast, within SAGA, Tra1 specifically promotes the incorporation of the de-ubiquitination module (DUB), defining an ordered assembly pathway. Biochemical and functional analyses elucidated the mechanism by which Tra1 assemble differentially into SAGA or NuA4 and identified a small, conserved region of Spt20 that is both necessary and sufficient to anchor Tra1 within SAGA. Finally, we establish that Hsp90 and its cochaperone TTT are required for Tra1 de novo incorporation into both SAGA and NuA4, indicating that Tra1, a pseudokinase of the PIKK family, shares a dedicated chaperone machinery with its cognate kinases. Overall, our work brings mechanistic insights into the de novo assembly of transcriptional complexes through ordered pathways and reveals the contribution of dedicated chaperones to this process.


1979 ◽  
Author(s):  
William L. Warnick ◽  
Garvin D. Chastain ◽  
William H. Ton

1959 ◽  
Author(s):  
Charles A. Baker ◽  
Dominic F. Morris ◽  
William C. Steedman
Keyword(s):  

Author(s):  
Atsuko Shirai ◽  
Akihisa Matsuyama ◽  
Yoko Yashiroda ◽  
Ritsuko Arai ◽  
Minoru Yoshida

2020 ◽  
pp. 1-12
Author(s):  
Changxin Sun ◽  
Di Ma

In the research of intelligent sports vision systems, the stability and accuracy of vision system target recognition, the reasonable effectiveness of task assignment, and the advantages and disadvantages of path planning are the key factors for the vision system to successfully perform tasks. Aiming at the problem of target recognition errors caused by uneven brightness and mutations in sports competition, a dynamic template mechanism is proposed. In the target recognition algorithm, the correlation degree of data feature changes is fully considered, and the time control factor is introduced when using SVM for classification,At the same time, this study uses an unsupervised clustering method to design a classification strategy to achieve rapid target discrimination when the environmental brightness changes, which improves the accuracy of recognition. In addition, the Adaboost algorithm is selected as the machine learning method, and the algorithm is optimized from the aspects of fast feature selection and double threshold decision, which effectively improves the training time of the classifier. Finally, for complex human poses and partially occluded human targets, this paper proposes to express the entire human body through multiple parts. The experimental results show that this method can be used to detect sports players with multiple poses and partial occlusions in complex backgrounds and provides an effective technical means for detecting sports competition action characteristics in complex backgrounds.


2019 ◽  
Author(s):  
Maria Teresa Odinolfi ◽  
Alessandro Romanato ◽  
Greta Bergamaschi ◽  
Alessandro Strada ◽  
Laura Sola ◽  
...  

The use of peptides in paper-based analytics is a highly appealing field, yet it suffers from severe limitations. This is mostly due to the loss of effective target recognition properties of this relatively small bioprobes upon nonspecific adsorption onto cellulose substrates. Here, we address this issue by introducing a simple polymer-based strategy to obtain clickable cellulosic surfaces, that we exploited for the chemoselective bioconjugation of peptide bioprobes. Our method largely outperformed standard adsorption-based immobilization strategy in a challenging, real-case immunoassay, namely the diagnostic discrimination of Zika+ individuals from healthy controls. Of note, the clickable polymeric coating not only allows efficient peptides bioconjugation, but it provides favorable anti-fouling properties to the cellulosic support. We envisage our strategy to broaden the repertoire of cellulosic materials manipulation and promote a renewed interest in peptide-based paper bioassays.


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