2021 Frank Stinchfield Award: A novel cemented hip hemiarthroplasty infection model with real-time in vivo imaging in rats

2021 ◽  
Vol 103-B (7 Supple B) ◽  
pp. 9-16
Author(s):  
William J. Hadden ◽  
Mazen Ibrahim ◽  
Mariam Taha ◽  
Kerstin Ure ◽  
Yun Liu ◽  
...  
Keyword(s):  
Real Time ◽  
In Vivo Imaging ◽  
Biofilm Formation ◽  
Joint Infection ◽  
Volumetric Analysis ◽  
In Vivo Model ◽  
Infection Model ◽  
Sprague Dawley ◽  
Hip Hemiarthroplasty

Aims The aims of this study were to develop an in vivo model of periprosthetic joint infection (PJI) in cemented hip hemiarthroplasty, and to monitor infection and biofilm formation in real-time. Methods Sprague-Dawley rats underwent cemented hip hemiarthroplasty via the posterior approach with pre- and postoperative gait assessments. Infection with Staphylococcus aureus Xen36 was monitored with in vivo photoluminescent imaging in real-time. Pre- and postoperative gait analyses were performed and compared. Postmortem micro (m) CT was used to assess implant integration; field emission scanning electron microscopy (FE-SEM) was used to assess biofilm formation on prosthetic surfaces. Results All animals tolerated surgery well, with preservation of gait mechanics and weightbearing in control individuals. Postoperative in vivo imaging demonstrated predictable evolution of infection with logarithmic signal decay coinciding with abscess formation. Postmortem mCT qualitative volumetric analysis showed high contact area and both cement-bone and cement-implant interdigitation. FE-SEM revealed biofilm formation on the prosthetic head. Conclusion This study demonstrates the utility of a new, high-fidelity model of in vivo PJI using cemented hip hemiarthroplasty in rats. Inoculation with bioluminescent bacteria allows for non-invasive, real-time monitoring of infection. Cite this article: Bone Joint J 2021;103-B(7 Supple B):9–16.

scholarly journals Direct Continuous Method for Monitoring Biofilm Infection in a Mouse Model

2003 ◽  
Vol 71 (2) ◽  
pp. 882-890 ◽  
Author(s):  
Jagath L. Kadurugamuwa ◽  
Lin Sin ◽  
Eddie Albert ◽  
Jun Yu ◽  
Kevin Francis ◽  
...  
Keyword(s):  
Real Time ◽  
Physiological State ◽  
Light Output ◽  
Drug Efficacy ◽  
Continuous Method ◽  
In Vivo Model ◽  
Infection Model ◽  
Mouse Infection Model

ABSTRACT We have developed a rapid, continuous method for real-time monitoring of biofilms, both in vitro and in a mouse infection model, through noninvasive imaging of bioluminescent bacteria colonized on Teflon catheters. Two important biofilm-forming bacterial pathogens, Staphylococcus aureus and Pseudomonas aeruginosa, were made bioluminescent by insertion of a complete lux operon. These bacteria produced significant bioluminescent signals for both in vitro studies and the development of an in vivo model, allowing effective real-time assessment of the physiological state of the biofilms. In vitro viable counts and light output were parallel and highly correlated (S. aureus r = 0.98; P. aeruginosa r = 0.99) and could be maintained for 10 days or longer, provided that growth medium was replenished every 12 h. In the murine model, subcutaneous implantation of the catheters (precolonized or postimplant infected) was well tolerated. An infecting dose of 10 3 to 10 5 CFU/catheter for S. aureus and P. aeruginosa resulted in a reproducible, localized infection surrounding the catheter that persisted until the termination of the experiment on day 20. Recovery of the bacteria from the catheters of infected animals showed that the bioluminescent signal corresponded to the CFU and that the lux constructs were highly stable even after many days in vivo. Since the metabolic activity of viable cells could be detected directly on the support matrix, nondestructively, and noninvasively, this method is especially appealing for the study of chronic biofilm infections and drug efficacy studies in vivo.

scholarly journals A New Antibiotic-Loaded Sol-Gel Can Prevent Bacterial Prosthetic Joint Infection: From in vitro Studies to an in vivo Model

2020 ◽  
Vol 10 ◽  
Author(s):  
John Jairo Aguilera-Correa ◽  
Amaya Garcia-Casas ◽  
Aranzazu Mediero ◽  
David Romera ◽  
Francisca Mulero ◽  
...  
Keyword(s):  
Prosthetic Joint Infection ◽  
Joint Infection ◽  
Sol Gel ◽  
In Vitro Studies ◽  
In Vivo Model ◽  
Prosthetic Joint

In vivo imaging of early stage apoptosis by measuring real-time caspase-3/7 activation

APOPTOSIS ◽  
2010 ◽  
Vol 16 (2) ◽  
pp. 198-207 ◽  
Author(s):  
Matteo Scabini ◽  
Fabio Stellari ◽  
Paolo Cappella ◽  
Sara Rizzitano ◽  
Gemma Texido ◽  
...  
Keyword(s):  
Real Time ◽  
In Vivo Imaging ◽  
Caspase 3 ◽  
Early Stage

scholarly journals Ultrasound and Transcriptomics Identify a Differential Impact of Cisplatin and Histone Deacetylation on Tumor Structure and Microenvironment in a Patient-Derived In Vivo Model of Gastric Cancer

Pharmaceutics ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1485
Author(s):  
Aina Venkatasamy ◽  
Eric Guerin ◽  
Anais Blanchet ◽  
Christophe Orvain ◽  
Véronique Devignot ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Tumor Microenvironment ◽  
Real Time ◽  
Histone Deacetylase Inhibitors ◽  
Structural Changes ◽  
Histone Deacetylation ◽  
In Vivo Model ◽  
Specific Cell ◽  
The Impact

The reasons behind the poor efficacy of transition metal-based chemotherapies (e.g., cisplatin) or targeted therapies (e.g., histone deacetylase inhibitors, HDACi) on gastric cancer (GC) remain elusive and recent studies suggested that the tumor microenvironment could contribute to the resistance. Hence, our objective was to gain information on the impact of cisplatin and the pan-HDACi SAHA (suberanilohydroxamic acid) on the tumor substructure and microenvironment of GC, by establishing patient-derived xenografts of GC and a combination of ultrasound, immunohistochemistry, and transcriptomics to analyze. The tumors responded partially to SAHA and cisplatin. An ultrasound gave more accurate tumor measures than a caliper. Importantly, an ultrasound allowed a noninvasive real-time access to the tumor substructure, showing differences between cisplatin and SAHA. These differences were confirmed by immunohistochemistry and transcriptomic analyses of the tumor microenvironment, identifying specific cell type signatures and transcription factor activation. For instance, cisplatin induced an “epithelial cell like” signature while SAHA favored a “mesenchymal cell like” one. Altogether, an ultrasound allowed a precise follow-up of the tumor progression while enabling a noninvasive real-time access to the tumor substructure. Combined with transcriptomics, our results underline the different intra-tumoral structural changes caused by both drugs that impact differently on the tumor microenvironment.

Real-time in vivo imaging of transgenic bioluminescent blood stages of rodent malaria parasites in mice

2006 ◽  
Vol 1 (1) ◽  
pp. 476-485 ◽  
Author(s):  
Blandine Franke-Fayard ◽  
Andrew P Waters ◽  
Chris J Janse
Keyword(s):  
Real Time ◽  
In Vivo Imaging ◽  
Malaria Parasites ◽  
Rodent Malaria

Biofilm formation on zirconia and titanium over time—An in vivo model study

2020 ◽  
Vol 31 (9) ◽  
pp. 865-880 ◽  
Author(s):  
Anton Desch ◽  
Nadine Freifrau von Maltzahn ◽  
Nico Stumpp ◽  
Marly Dalton ◽  
Ines Yang ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
In Vivo Model ◽  
Model Study ◽  
Over Time

scholarly journals Synergistic toxicity in an in vivo model of neurodegeneration through the co-expression of human TDP-43M337V and tauT175D protein

2019 ◽  
Vol 7 (1) ◽  
Author(s):  
Alexander J. Moszczynski ◽  
Madeline Harvey ◽  
Niveen Fulcher ◽  
Cleusa de Oliveira ◽  
Patrick McCunn ◽  
...  
Keyword(s):  
Tau Protein ◽  
Sensorimotor Gating ◽  
In Vivo Model ◽  
Motor Deficits ◽  
Sprague Dawley ◽  
Tau Pathology ◽  
Adult Rats ◽  
Sprague Dawley Rats ◽  
Somatic Gene Transfer

Abstract Although it has been suggested that the co-expression of multiple pathological proteins associated with neurodegeneration may act synergistically to induce more widespread neuropathology, experimental evidence of this is sparse. We have previously shown that the expression of Thr175Asp-tau (tauT175D) using somatic gene transfer with a stereotaxically-injected recombinant adeno-associated virus (rAAV9) vector induces tau pathology in rat hippocampus. In this study, we have examined whether the co-expression of human tauT175D with mutant human TDP-43 (TDP-43M337V) will act synergistically. Transgenic female Sprague-Dawley rats that inducibly express mutant human TDP-43M337V using the choline acetyltransferase (ChAT) tetracycline response element (TRE) driver with activity modulating tetracycline-controlled transactivator (tTA) were utilized in these studies. Adult rats were injected with GFP-tagged tau protein constructs in a rAAV9 vector through bilateral stereotaxic injection into the hippocampus. Injected tau constructs were: wild-type GFP-tagged 2N4R human tau (tauWT; n = 8), GFP-tagged tauT175D 2N4R human tau (tauT175D, pseudophosphorylated, toxic variant, n = 8), and GFP (control, n = 8). Six months post-injection, mutant TDP-43M337V expression was induced for 30 days. Behaviour testing identified motor deficits within 3 weeks after TDP-43 expression irrespective of tau expression, though social behaviour and sensorimotor gating remained unchanged. Increased tau pathology was observed in the hippocampus of both tauWT and tauT175D expressing rats and tauT175D pathology was increased in the presence of cholinergic neuronal expression of human TDP-43M337V. These data indicate that co-expression of pathological TDP-43 and tau protein exacerbate the pathology associated with either individual protein.

scholarly journals Real-time in vivo imaging of regional lung function in a mouse model of cystic fibrosis on a laboratory X-ray source

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Rhiannon P. Murrie ◽  
Freda Werdiger ◽  
Martin Donnelley ◽  
Yu-wei Lin ◽  
Richard P. Carnibella ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Lung Function ◽  
Mouse Model ◽  
Real Time ◽  
In Vivo Imaging ◽  
X Ray ◽  
Regional Lung Function ◽  
Regional Lung

Real-time in vivo imaging by high-speed spectral optical coherence tomography

2003 ◽  
Vol 28 (19) ◽  
pp. 1745 ◽  
Author(s):  
Maciej Wojtkowski ◽  
Tomasz Bajraszewski ◽  
Piotr Targowski ◽  
Andrzej Kowalczyk
Keyword(s):  
Optical Coherence Tomography ◽  
Real Time ◽  
In Vivo Imaging ◽  
High Speed ◽  
Optical Coherence ◽  
Spectral Optical Coherence Tomography
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