scholarly journals Author response: Ribosome recycling is not critical for translational coupling in Escherichia coli

2020 ◽  
Author(s):  
Kazuki Saito ◽  
Rachel Green ◽  
Allen R Buskirk
1998 ◽  
Vol 180 (19) ◽  
pp. 5240-5242 ◽  
Author(s):  
Donald Oliver ◽  
Jessica Norman ◽  
Shameema Sarker

ABSTRACT secA is translationally regulated by the protein secretion proficiency state of the Escherichia coli cell. This regulation was explored by making signal sequence mutations in the gene upstream of secA, gene X, which promotessecA translational coupling. Gene X signal sequence mutants were constitutive for secA expression, whileprlA alleles partially restored secAregulation. These results show that interaction of the pre-gene X protein with the translocon is required for proper secAregulation. Furthermore, gene X signal sequence mutations disruptedsecA regulation only in the cis configuration. We propose that nascent pre-gene X protein interacts with the translocon during its secretion to constitute the secretion sensor.


Author(s):  
Michael Luke Carlson ◽  
R Greg Stacey ◽  
John William Young ◽  
Irvinder Singh Wason ◽  
Zhiyu Zhao ◽  
...  

Author(s):  
Mehdi Snoussi ◽  
John Paul Talledo ◽  
Nathan-Alexander Del Rosario ◽  
Salimeh Mohammadi ◽  
Bae-Yeun Ha ◽  
...  

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