scholarly journals Role of Gastric Stem Cells in Gastric Carcinogenesis by ChronicHelicobacter pyloriInfection

2015 ◽  
Vol 15 (3) ◽  
pp. 155
Author(s):  
Moo-In Park ◽  
Jeonghoon Heo
Keyword(s):  
Stem Cells ◽  
Gastric Carcinogenesis ◽  
Gastric Stem Cells

scholarly journals Expression of Angiogenin is inversely correlated to Progression of Gastric Tumors

2019 ◽  
pp. 120-136
Author(s):  
Yunxia Wu ◽  
Muntadher Al-sarraf ◽  
Huang Xiao-Tao ◽  
Zhang Zhi-qiang ◽  
Li Bin ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Stem Cells ◽  
Tumor Stage ◽  
Gastric Mucus ◽  
Adjacent Tissue ◽  
Glandular Cells ◽  
Differentiation Status ◽  
Cancer Tissues ◽  
Gastric Stem Cells

Objective: Angiogenin (ANG) is upregulated in a variety of cancers including those of prostate, cervix, pancreas, liver, oral cavity, skin, and etc., however, the role of ANG in gastric cancer has not been fully elucidated yet. We use tissue microarray (TMA) to examine ANG expression to investigate the role of ANG in the progression of gastric cancer. Method: Immunohistochemistry was used to evaluate ANG expression in TMA with 208 spots from 104 patients diagnosed with gastric cancer and the corresponding adjacent tissue. Results: In normal adjacent tissue, ANG was expressed mainly in cytoplasm at basal gland of the gastric mucus where gastric stem cells are reserved, and also sparsely expressed in the nucleus of gastric mucosal gland cells at isthmus where gastric stem cells are gathered. In cancer tissues, ANG was very sparsely expressed in the nucleus of gastric glandular cells. ANG expression in the cytoplasm was found to be significantly associated with pathological types (p<0.001) and malignancy (p<0.001). ANG expression in the nucleus was inversely correlated with malignancy (p=0.019), differentiation status (p< 0.001), and tumor stage (p= 0.048).Conclusion: ANG might play an important role in gastric cancer development.

In Vitro Evidence of the Role of COX-2 in Attenuating Gastric Inflammation and Promoting Gastric Carcinogenesis

2002 ◽  
Vol 21 (2) ◽  
pp. 12 ◽  
Author(s):  
Ki-Baik Hahm ◽  
Ho-Yeong Lim ◽  
Seonghyang Sohn ◽  
Hyuk-Jae Kwon ◽  
Ki-Myung Lee ◽  
...  
Keyword(s):  
Gastric Carcinogenesis ◽  
Cox 2

An Investigation Of The Role Of Stem Cells In Explaining Bodily Resurrection

2018 ◽  
pp. 41
Author(s):  
قدسية اکبري ◽  
سید مرتضی حسینی شاھرودي ◽  
أفضل بلوكى ◽  
مرضية آبیاري
Keyword(s):  
Stem Cells ◽  
Bodily Resurrection

Therapeutic Role of Bmi-1 Inhibitors in Eliminating Prostate Tumor Stem Cells

2013 ◽  
Author(s):  
Isaac Y. Kim ◽  
Joseph Bertino ◽  
Hatem E. Sabaawy
Keyword(s):  
Stem Cells ◽  
Prostate Tumor ◽  
Tumor Stem Cells ◽  
Therapeutic Role

Overexpression of Pygo2 Increases Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Cardiomyocyte-like Cells

2020 ◽  
Vol 20 (4) ◽  
pp. 318-324 ◽  
Author(s):  
Lei Yang ◽  
Shuoji Zhu ◽  
Yongqing Li ◽  
Jian Zhuang ◽  
Jimei Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Heart Function ◽  
Critical Role ◽  
Human Umbilical Cord ◽  
Stem Cell Markers ◽  
Quantitative Real Time Pcr ◽  
Qrt Pcr

Background: Our previous studies have shown that Pygo (Pygopus) in Drosophila plays a critical role in adult heart function that is likely conserved in mammals. However, its role in the differentiation of human umbilical cord mesenchymal stem cells (hUC-MSCs) into cardiomyocytes remains unknown. Objective: To investigate the role of pygo2 in the differentiation of hUC-MSCs into cardiomyocytes. Methods: Third passage hUC-MSCs were divided into two groups: a p+ group infected with the GV492-pygo2 virus and a p− group infected with the GV492 virus. After infection and 3 or 21 days of incubation, Quantitative real-time PCR (qRT-PCR) was performed to detect pluripotency markers, including OCT-4 and SOX2. Nkx2.5, Gata-4 and cTnT were detected by immunofluorescence at 7, 14 and 21 days post-infection, respectively. Expression of cardiac-related genes—including Nkx2.5, Gata-4, TNNT2, MEF2c, ISL-1, FOXH1, KDR, αMHC and α-Actin—were analyzed by qRT-PCR following transfection with the virus at one, two and three weeks. Results : After three days of incubation, there were no significant changes in the expression of the pluripotency stem cell markers OCT-4 and SOX2 in the p+ group hUC-MSCs relative to controls (OCT-4: 1.03 ± 0.096 VS 1, P > 0.05, SOX2: 1.071 ± 0.189 VS 1, P > 0.05); however, after 21 days, significant decreases were observed (OCT-4: 0.164 ± 0.098 VS 1, P < 0.01, SOX2: 0.209 ± 0.109 VS 1, P < 0.001). Seven days following incubation, expression of mesoderm specialisation markers, such as Nkx2.5, Gata-4, MEF2c and KDR, were increased; at 14 days following incubation, expression of cardiac genes, such as Nkx2.5, Gata-4, TNNT2, MEF2c, ISL-1, FOXH1, KDR, αMHC and α-Actin, were significantly upregulated in the p+ group relative to the p− group (P < 0.05). Taken together, these findings suggest that overexpression of pygo2 results in more hUCMSCs gradually differentiating into cardiomyocyte-like cells. Conclusion: We are the first to show that overexpression of pygo2 significantly enhances the expression of cardiac-genic genes, including Nkx2.5 and Gata-4, and promotes the differentiation of hUC-MSCs into cardiomyocyte-like cells.

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