scholarly journals Inhibition of Kelch-like epichlorohydrin-related protein 1 promotes the progression and drug resistance of lung adenocarcinoma

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11908
Author(s):  
Hong Gao ◽  
Peipei Tang ◽  
Kejie Ni ◽  
Lun Zhu ◽  
Song Chen ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Proliferation ◽  
Drug Resistance ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Lung Adenocarcinoma ◽  
Chemotherapy Resistance ◽  
Related Protein ◽  
H1299 Cell ◽  
Node Metastasis

Background Lung cancer is a common malignant carcinoma of respiratory system with high morbidity and mortality. Kelch-like epichlorohydrin-related protein 1 (Keap1), a member of the BTB-Kelch protein family, has been reported as an important molecule in several cancers. However, its potential role in tumor is still controversial. Here we aim to clarify the effect of Keap1 on the biological characteristics and chemotherapy resistance in lung adenocarcinoma (LUAD). Methods Immunohistochemistry was conducted to compare Keap1 expression in lung adenocarcinoma tissues and matched non-cancerous tissues, and the correlation between Keap1 expression and clinicopathological features was analyzed. Subsequently, the stable A549 and H1299 cell lines with Keap1 knockdown or overexpression were constructed using lentivirus. The roles of Keap1 on the cell proliferation, migration, invasion and drug resistance were investigated by colony formation assay, cell proliferation assay, wound scratch test, transwell invasion assay and drug sensitivity assay, respectively. Results Keap1 was lowly expressed in tumor tissues compared to matched non-cancerous tissues, and its expression was correlated with TNM stage and lymph node metastasis. Early stage (I) tumors without lymph node metastasis had higher levels of Keap1 expression compared with late-stage tumors (II, III) with the presence of lymphatic metastasis. Colony formation assays showed that Keap1 knockdown promoted the proliferation of A549 and H1299 cells, and the cell growth curves further confirmed this feature. In contrast, wound scratch and transwell invasion experiments showed that Keap1 overexpression inhibited cell migration and invasive malignancy. The IC50 for cisplatin and paclitaxel were significantly increased by Keap1 knockdown in A549 and H1299 cell lines. Conclusion Keap1 knockdown promotes tumor cell growth, proliferation, invasion, metastasis and chemotherapy resistance in LUAD. It may be a potential tumor marker to guide the staging and treatment of lung cancer.

scholarly journals Microarray Identifies a Key Carcinogenic Circular RNA 0008594 That Is Related to Non-Small-Cell Lung Cancer Development and Lymph Node Metastasis and Promotes NSCLC Progression by Regulating the miR-760-Mediated PI3K/AKT and MEK/ERK Pathways

2021 ◽  
Vol 11 ◽  
Author(s):  
Qiushi Wang ◽  
Chunhua Yan ◽  
Pengfei Zhang ◽  
Guanghua Li ◽  
Ruidong Zhu ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Proliferation ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Small Cell Lung Cancer ◽  
Circular Rna ◽  
Small Cell ◽  
Small Cell Lung ◽  
Node Metastasis ◽  
H460 Cell

PurposeThis study aimed to explore the circular RNA (circRNA/circ) profile engaged in non-small cell lung cancer (NSCLC) development and metastasis and to investigate potentially key carcinogenic circRNAs related to NSCLC.MethodsCircRNA profiles between 10 NSCLC tissues and 10 adjacent tissues and between five NSCLC tissues with lymph node metastasis (LNM) and five NSCLC tissues without LNM were detected by Arraystar Human circRNA Array followed by bioinformatics. Circ_0008594 knockdown, circ_0004293 overexpression, and circ_0003832 overexpression plasmids were transfected into H23 and H460 cells to sort potential oncogenic circRNA. Then circ_0008594 overexpression and knockdown plasmids were transfected, followed by that circ_0008594 knockdown plus miR-760 knockdown plasmids were transfected into these cells. Cell proliferation, apoptosis, invasion, stemness, and pathways were detected. In addition, xenograft mice models were constructed via injecting H23 cells with circ_0008594 overexpression or knockdown to validate the findings.ResultsA total of 455 dysregulated circRNAs in NSCLC tissues versus adjacent tissues and 353 dysregulated circRNAs in NSCLC tissues with LNM versus those without LNM were discovered. Via cross-analysis, 19 accordant circRNAs were uncovered, among which three candidate circRNAs (circ_0008594, circ_0004293, circ_0003832) were chosen for functional experiments, during which it was observed that circ_0008549 affected H23 and H460 cell proliferation and apoptosis more obviously than circ_0004293 and circ_0003832. Subsequent experiments showed that circ_0008594 promoted H23 and H460 cell proliferation and invasion but affected stemness less and negatively regulated miR-760 via direct binding. Furthermore, miR-760 attenuated the effect of circ_0008549 on regulating H23 and H460 cell functions and the PI3K/AKT and MEK/ERK pathways. In vivo experiments further confirmed that circ_0008549 increased tumor volume, epithelial-mesenchymal transition, and the PI3K/AKT and MEK/ERK pathways while reducing tumor apoptosis and miR-760 NSCLC xenograft models.ConclusionOur study identifies several valuable circRNAs related to NSCLC development and LNM. Furthermore, as a key functional circRNA, circ_0008594 was observed to promote NSCLC progression by regulating the miR-760-mediated PI3K/AKT and MEK/ERK pathways.

Evaluation of Mediastinal Lymph Node Metastasis in Lung Cancer: Factors influencing the Diagnostic Accuracy ofCT

1998 ◽  
Vol 38 (3) ◽  
pp. 445
Author(s):  
Du Hwan Choe ◽  
Tae Hyun Lee ◽  
Kie Hwan Kim ◽  
Soo Yil Chin ◽  
Jae Ill Zo ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Diagnostic Accuracy ◽  
Mediastinal Lymph Node ◽  
Mediastinal Lymph Node Metastasis ◽  
Node Metastasis ◽  
Factors Influencing

scholarly journals Intraoperative diagnosis of lymph node metastasis during segmentectomy for non‐small cell lung cancer by rapid immunohistochemistry using noncontact alternating current electric field mixing

2020 ◽  
Vol 11 (12) ◽  
pp. 3547-3554
Author(s):  
Kazuhiro Imai ◽  
Hiroshi Nanjo ◽  
Shinogu Takashima ◽  
Yuko Hiroshima ◽  
Maiko Atari ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Electric Field ◽  
Small Cell Lung Cancer ◽  
Small Cell ◽  
Small Cell Lung ◽  
Intraoperative Diagnosis ◽  
Node Metastasis ◽  
Current Electric Field

Increased high-mobility group A2 correlates with lymph node metastasis and prognosis of non-small cell lung cancer

2018 ◽  
Vol 21 (3) ◽  
pp. 547-555 ◽  
Author(s):  
Xiangjun Guo ◽  
Jiaxin Shi ◽  
Yan Wen ◽  
Mengmeng Li ◽  
Qin Li ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
High Mobility ◽  
Small Cell ◽  
High Mobility Group ◽  
Small Cell Lung ◽  
Node Metastasis

Primary lung cancer after treatment of head and neck cancer without lymph node metastasis: Is there a role for autofluorescence bronchoscopy?

Lung Cancer ◽  
2008 ◽  
Vol 62 (3) ◽  
pp. 309-315 ◽  
Author(s):  
Pyng Lee ◽  
Remco de Bree ◽  
Hes A.P. Brokx ◽  
C. René Leemans ◽  
Pieter E. Postmus ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Head And Neck Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Head And Neck ◽  
Neck Cancer ◽  
Primary Lung Cancer ◽  
Node Metastasis ◽  
Autofluorescence Bronchoscopy

Long form collapsin response mediator protein-1 (LCRMP-1) expression is associated with clinical outcome and lymph node metastasis in non-small cell lung cancer patients

Lung Cancer ◽  
2010 ◽  
Vol 67 (1) ◽  
pp. 93-100 ◽  
Author(s):  
Szu-Hua Pan ◽  
Yu-Chih Chao ◽  
Hsuan-Yu Chen ◽  
Pei-Fang Hung ◽  
Pei-Ying Lin ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Small Cell ◽  
Small Cell Lung ◽  
Collapsin Response Mediator Protein ◽  
Lung Cancer Patients ◽  
Node Metastasis ◽  
Mediator Protein ◽  
Long Form

scholarly journals Expression of CXCR4 and VEGF-C is correlated with lymph node metastasis in non-small cell lung cancer

2017 ◽  
Vol 8 (6) ◽  
pp. 634-641 ◽  
Author(s):  
Ming Ming Bi ◽  
Bin Shang ◽  
Zhou Wang ◽  
Gang Chen
Keyword(s):  
Lung Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Small Cell ◽  
Small Cell Lung ◽  
Node Metastasis
Sign in / Sign up

Export Citation Format

Share Document