Improved isolation and detection of toxigenic Vibrio parahaemolyticus from coastal water in Saudi Arabia using immunomagnetic enrichment

Background Vibrio parahaemolyticus is recognized globally as a cause of foodborne gastroenteritis and its widely disseminated in marine and coastal environment throughout the world. The main aim of this study was conducted to investigate the presence of toxigenic V. parahaemolyticus in costal water in the Eastern Province of Saudi Arabia by using immunomagnetic separation (IMS) in combination with chromogenic Vibrio agar medium and PCR targeting toxR gene of species level and virulence genes. Methods A total of 192 seawater samples were collected from five locations and enriched in alkaline peptone water (APW) broth. One-milliliter portion from enriched samples in APW were mixed with an immunomagnetic beads (IMB) coated with specific antibodies against V. parahaemolyticus polyvalent K antisera and separated beads with captured bacteria streaked on thiosulfate citrate bile salts sucrose (TCBS) agar and CHROMagar Vibrio (CaV) medium. Results Of the 192 examined seawater samples, 38 (19.8%) and 44 (22.9%) were positive for V. parahaemolyticus, producing green and mauve colonies on TCBS agar and CaV medium, respectively. Among 120 isolates of V. parahaemolyticus isolated in this study, 3 (2.5%) and 26 (21.7%) isolates of V. parahaemolyticus isolated without and with IMB treatment tested positive for the toxin regulatory (toxR) gene, respectively. Screening of the confirmed toxR gene-positive isolates revealed that 21 (17.5%) and 3 (2.5%) were positive for the thermostable direct hemolysin (tdh) encoding gene in strains isolated with IMB and without IMB treatment, respectively. None of the V. parahaemolyticus strains tested positive for the thermostable related hemolysin (trh) gene. In this study, we found that the CaV medium has no advantage over TCBS agar if IMB concentration treatment is used during secondary enrichment steps of environmental samples. The enterobacterial repetitive intergenic consensus (ERIC)-PCR DNA fingerprinting analysis revealed high genomic diversity, and 18 strains of V. parahaemolyticus were grouped and identified into four identical ERIC clonal group patterns. Conclusions The presented study reports the first detection of tdh producing V. parahaemolyticus in coastal water in the Eastern Province of Saudi Arabia.

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Characterization of Vibrio parahaemolyticus isolated from coastal water in Eastern Province of Saudi Arabia

Malaysian Journal of Microbiology ◽

10.21161/mjm.96316 ◽

2018 ◽

Author(s):

Elhadi, N., ◽

Nishibuchi, M.

Keyword(s):

Saudi Arabia ◽

Coastal Water ◽

Vibrio Parahaemolyticus ◽

Eastern Province

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Occurrence of Potentially Human Pathogenic Vibrio Species in the Coastal Water of the Eastern Province of Saudi Arabia

Research Journal of Microbiology ◽

10.3923/jm.2013.1.12 ◽

2013 ◽

Vol 8 (1) ◽

pp. 1-12 ◽

Cited By ~ 1

Author(s):

Nasreldin Elhadi

Keyword(s):

Saudi Arabia ◽

Coastal Water ◽

Vibrio Species ◽

Eastern Province ◽

Pathogenic Vibrio

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Clonal relationship among the Vibrio parahaemolyticus isolates from coastal water in Saudi Arabia

The Egyptian Journal of Aquatic Research ◽

10.1016/j.ejar.2018.04.002 ◽

2018 ◽

Vol 44 (2) ◽

pp. 131-137 ◽

Cited By ~ 1

Author(s):

Nasreldin Elhadi

Keyword(s):

Saudi Arabia ◽

Coastal Water ◽

Vibrio Parahaemolyticus ◽

Clonal Relationship

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Distribution of Virulent and Pandemic Strains of Vibrio parahaemolyticus in Three Molluscan Shellfish Species (Meretrix meretrix, Perna viridis, and Anadara granosa) and Their Association with Foodborne Disease in Southern Thailand

Journal of Food Protection ◽

10.4315/0362-028x-69.11.2615 ◽

2006 ◽

Vol 69 (11) ◽

pp. 2615-2620 ◽

Cited By ~ 21

Author(s):

VARAPORN VUDDHAKUL ◽

SUPATINEE SOBOON ◽

WATTANEE SUNGHIRAN ◽

SUKHON KAEWPIBOON ◽

ASHRAFUZZAMAN CHOWDHURY ◽

...

Keyword(s):

Vibrio Parahaemolyticus ◽

Immunomagnetic Separation ◽

Hard Clam ◽

Perna Viridis ◽

Clinical Strains ◽

Specific Pcr ◽

Alkaline Peptone Water ◽

Southern Thailand ◽

Peptone Water ◽

Pandemic Strains

Distribution of pandemic strains of Vibrio parahaemolyticus in seafood, particularly in molluscan shellfish, and their serological and molecular relationships to clinical strains were examined from Hat Yai City in southern Thailand. During 2000 to 2002, virulent strains (tdh+ or trh+) were isolated from 13 of 230 molluscan shellfish samples using alkaline peptone water enrichment followed by immunomagnetic separation. The isolates included 12 pandemic strains (tdh+, trh−, group-specific PCR positive) from five Oriental hard clam samples, five green mussel samples, and one bloody clam sample. Among the pandemic strains, eight belonged to serogroup O3:K6, three belonged to O1:K25, and one was O1:K untypeable. One hundred eighty-seven strains of V. parahaemolyticus were isolated from clinical specimens obtained from a hospital in this city during 2000 to 2001. The pandemic strains comprised 64 and 68% of the isolates in 2000 and 2001, respectively. Among the serotypes of the pandemic strains, O3:K6 was dominant at 73% in 2000 and 76% in 2001 followed by O1:K25 at 20% in 2000 and 13% in 2001. Pulsed-field gel electrophoresis profiles of the pandemic strains from molluscan shellfish were indistinguishable or very similar to those of patient isolates. Similarity of the serotype distribution and DNA fingerprints occurring between the molluscan shellfish strains and clinical strains suggests that molluscan shellfish may be an important source of pandemic V. parahaemolyticus infection in southern Thailand. For public health, proper cooking of molluscan shellfish in this area is strongly recommended.

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Suicidal Ideation Among Medical Students in Dammam, Saudi Arabia

Crisis ◽

10.1027/0227-5910/a000720 ◽

2020 ◽

pp. 1-6

Author(s):

Mohammed Madadin ◽

Ritesh G. Menezes ◽

Maha A. Alassaf ◽

Abdulaziz M. Almulhim ◽

Mahdi S. Abumadini ◽

...

Keyword(s):

Saudi Arabia ◽

Medical Students ◽

Suicidal Ideation ◽

General Health Questionnaire ◽

Cross Sectional Study ◽

Eastern Province ◽

Cross Sectional ◽

The Past ◽

Depression Subscale ◽

History Of

Abstract. Background: Medical students are at high risk of suicidal ideation. Aim: We aimed to obtain information on suicidal ideation among medical students in Dammam located in the Eastern Province of Saudi Arabia. Method: This cross-sectional study was conducted at the College of Medicine affiliated with Imam Abdulrahman Bin Faisal University in the Eastern Province of Saudi Arabia. Suicidal ideation in the past 12 months was assessed based on responses to four questions in the depression subscale of the General Health Questionnaire 28 (GHQ-28). In addition, data were collected to examine the association of suicidal ideation with various factors. Results: We found that 1 in 3 medical students in the study had suicidal ideation in the past 12 months, while around 40% had lifetime suicidal ideation. Suicidal ideation was associated with feelings of parental neglect, history of physical abuse, and dissatisfaction with academic performance. Limitations: The cross-sectional nature of this study limits its ability to determine causality regarding suicidal ideation. Conclusion: These rates are considerably high when compared with rates from studies in other countries around the world. This study provides a reference in the field of suicidology for this region of Saudi Arabia.

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Prevalence and Diversity of Haplotypes of Sickle Cell Disease in the Eastern Province of Saudi Arabia

Hemoglobin ◽

10.1080/03630269.2020.1739068 ◽

2020 ◽

Vol 44 (2) ◽

pp. 78-81 ◽

Cited By ~ 5

Author(s):

Amein K. Al-Ali ◽

Ahmed Alsulaiman ◽

Alhusain J. Alzahrani ◽

Obeid T. Obeid ◽

Chitti Babu Vatte ◽

...

Keyword(s):

Saudi Arabia ◽

Sickle Cell Disease ◽

Sickle Cell ◽

Cell Disease ◽

Eastern Province

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Molecular Identification of Trypanosoma evansi Isolated from Arabian Camels (Camelus dromedarius) in Riyadh and Al-Qassim, Saudi Arabia

Animals ◽

10.3390/ani11041149 ◽

2021 ◽

Vol 11 (4) ◽

pp. 1149

Author(s):

Dina M. Metwally ◽

Isra M. Al-Turaiki ◽

Najwa Altwaijry ◽

Samia Q. Alghamdi ◽

Abdullah D. Alanazi

Keyword(s):

Saudi Arabia ◽

Infection Rate ◽

Ribosomal Rna ◽

Phylogenetic Analyses ◽

Trypanosoma Evansi ◽

Camelus Dromedarius ◽

18S Ribosomal Rna ◽

18S Ribosomal Rna Gene ◽

Polymerase Chain ◽

Pcr Targeting

We analyzed the blood from 400 one-humped camels, Camelus dromedarius (C. dromedarius), in Riyadh and Al-Qassim, Saudi Arabia to determine if they were infected with the parasite Trypanosoma spp. Polymerase chain reaction (PCR) targeting the internal transcribed spacer 1 (ITS1) gene was used to detect the prevalence of Trypanosoma spp. in the camels. Trypanosoma evansi (T. evansi) was detected in 79 of 200 camels in Riyadh, an infection rate of 39.5%, and in 92 of 200 camels in Al-Qassim, an infection rate of 46%. Sequence and phylogenetic analyses revealed that the isolated T. evansi was closely related to the T. evansi that was detected in C. dromedarius in Egypt and the T. evansi strain B15.1 18S ribosomal RNA gene identified from buffalo in Thailand. A BLAST search revealed that the sequences are also similar to those of T. evansi from beef cattle in Thailand and to T. brucei B8/18 18S ribosomal RNA from pigs in Nigeria.

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