scholarly journals Characterization of Vibrio parahaemolyticus isolated from coastal water in Eastern Province of Saudi Arabia

2018 ◽  
Author(s):  
Elhadi, N., ◽  
Nishibuchi, M.
Keyword(s):  
Saudi Arabia ◽  
Coastal Water ◽  
Vibrio Parahaemolyticus ◽  
Eastern Province

scholarly journals Improved isolation and detection of toxigenic Vibrio parahaemolyticus from coastal water in Saudi Arabia using immunomagnetic enrichment

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12402
Author(s):  
Mariam Almejhim ◽  
Mohammed Aljeldah ◽  
Nasreldin Elhadi
Keyword(s):  
Saudi Arabia ◽  
Coastal Water ◽  
Vibrio Parahaemolyticus ◽  
Immunomagnetic Separation ◽  
Genomic Diversity ◽  
R Gene ◽  
Eastern Province ◽  
Alkaline Peptone Water ◽  
Seawater Samples ◽  
Pcr Targeting

Background Vibrio parahaemolyticus is recognized globally as a cause of foodborne gastroenteritis and its widely disseminated in marine and coastal environment throughout the world. The main aim of this study was conducted to investigate the presence of toxigenic V. parahaemolyticus in costal water in the Eastern Province of Saudi Arabia by using immunomagnetic separation (IMS) in combination with chromogenic Vibrio agar medium and PCR targeting toxR gene of species level and virulence genes. Methods A total of 192 seawater samples were collected from five locations and enriched in alkaline peptone water (APW) broth. One-milliliter portion from enriched samples in APW were mixed with an immunomagnetic beads (IMB) coated with specific antibodies against V. parahaemolyticus polyvalent K antisera and separated beads with captured bacteria streaked on thiosulfate citrate bile salts sucrose (TCBS) agar and CHROMagar Vibrio (CaV) medium. Results Of the 192 examined seawater samples, 38 (19.8%) and 44 (22.9%) were positive for V. parahaemolyticus, producing green and mauve colonies on TCBS agar and CaV medium, respectively. Among 120 isolates of V. parahaemolyticus isolated in this study, 3 (2.5%) and 26 (21.7%) isolates of V. parahaemolyticus isolated without and with IMB treatment tested positive for the toxin regulatory (toxR) gene, respectively. Screening of the confirmed toxR gene-positive isolates revealed that 21 (17.5%) and 3 (2.5%) were positive for the thermostable direct hemolysin (tdh) encoding gene in strains isolated with IMB and without IMB treatment, respectively. None of the V. parahaemolyticus strains tested positive for the thermostable related hemolysin (trh) gene. In this study, we found that the CaV medium has no advantage over TCBS agar if IMB concentration treatment is used during secondary enrichment steps of environmental samples. The enterobacterial repetitive intergenic consensus (ERIC)-PCR DNA fingerprinting analysis revealed high genomic diversity, and 18 strains of V. parahaemolyticus were grouped and identified into four identical ERIC clonal group patterns. Conclusions The presented study reports the first detection of tdh producing V. parahaemolyticus in coastal water in the Eastern Province of Saudi Arabia.

scholarly journals Serological and Molecular Characterization of Mycobacterium avium Subsp. paratuberculosis (MAP) from Sheep, Goats, Cattle and Camels in the Eastern Province, Saudi Arabia

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 323
Author(s):  
Ibrahim Elsohaby ◽  
Mahmoud Fayez ◽  
Mohamed Alkafafy ◽  
Mohamed Refaat ◽  
Theeb Al-Marri ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Molecular Characterization ◽  
Mycobacterium Avium ◽  
Mycobacterium Avium Subsp ◽  
Type I ◽  
Serum Samples ◽  
Eastern Province ◽  
Fecal Samples ◽  
Mycobacterium Avium Subsp Paratuberculosis

The objectives of the present study were to characterize Mycobacterium avium subsp. paratuberculosis (MAP) infection using serological and molecular tools and investigate the distribution and molecular characterization of MAP strains (cattle (C) and sheep (S) types) in sheep, goat, cattle, and camel herds in Eastern Province, Saudi Arabia. Serum and fecal samples were collected from all animals aged >2 years old in 31 herds (sheep = 8, goats = 6, cattle = 8 and camels = 9) from January to December 2019. Serum samples were tested by ELISA for the detection of MAP antibodies. Fecal samples were tested by PCR for the detection of MAP IS900 gene and the identification of MAP strains. MAP antibodies were detected in 19 (61.3%) herds. At the animal level, antibodies against MAP were detected in 43 (19.5%) sheep, 21 (17.1%) goats, 13 (19.7%) cattle and 22 (9.1%) camels. The IS900 gene of MAP was detected in 23 (74.2%) herds and was directly amplified from fecal samples of 59 (26.8%) sheep, 34 (27.6%) goats, 20 (30.3%) cattle and 36 (15.0%) camels. The S-type was the most prevalent MAP type identified in 15 herds, and all were identified as type-I, while the C-type was identified in only 8 herds. The IS900 sequences revealed genetic differences among the MAP isolates recovered from sheep, goats, cattle and camels. Results from the present study show that MAP was prevalent and confirm the distribution of different MAP strains in sheep, goat, cattle and camel herds in Eastern Province, Saudi Arabia.

scholarly journals Characterization of Vibrio vulnificus Isolated from the Coastal Areas in the Eastern Province of Saudi Arabia

2018 ◽  
Vol 12 (3) ◽  
pp. 1355-1364
Author(s):  
Zainab Alsalem ◽  
Nasreldin Elhadi ◽  
Mohammed Aljeldah ◽  
Faisal Alzahrani ◽  
Mitsuaki Nishibuchi
Keyword(s):  
Saudi Arabia ◽  
Vibrio Vulnificus ◽  
Coastal Areas ◽  
Eastern Province

Suicidal Ideation Among Medical Students in Dammam, Saudi Arabia

Crisis ◽  
2020 ◽  
pp. 1-6
Author(s):  
Mohammed Madadin ◽  
Ritesh G. Menezes ◽  
Maha A. Alassaf ◽  
Abdulaziz M. Almulhim ◽  
Mahdi S. Abumadini ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Medical Students ◽  
Suicidal Ideation ◽  
General Health Questionnaire ◽  
Cross Sectional Study ◽  
Eastern Province ◽  
Cross Sectional ◽  
The Past ◽  
Depression Subscale ◽  
History Of

Abstract. Background: Medical students are at high risk of suicidal ideation. Aim: We aimed to obtain information on suicidal ideation among medical students in Dammam located in the Eastern Province of Saudi Arabia. Method: This cross-sectional study was conducted at the College of Medicine affiliated with Imam Abdulrahman Bin Faisal University in the Eastern Province of Saudi Arabia. Suicidal ideation in the past 12 months was assessed based on responses to four questions in the depression subscale of the General Health Questionnaire 28 (GHQ-28). In addition, data were collected to examine the association of suicidal ideation with various factors. Results: We found that 1 in 3 medical students in the study had suicidal ideation in the past 12 months, while around 40% had lifetime suicidal ideation. Suicidal ideation was associated with feelings of parental neglect, history of physical abuse, and dissatisfaction with academic performance. Limitations: The cross-sectional nature of this study limits its ability to determine causality regarding suicidal ideation. Conclusion: These rates are considerably high when compared with rates from studies in other countries around the world. This study provides a reference in the field of suicidology for this region of Saudi Arabia.

Prevalence and Diversity of Haplotypes of Sickle Cell Disease in the Eastern Province of Saudi Arabia

Hemoglobin ◽  
2020 ◽  
Vol 44 (2) ◽  
pp. 78-81 ◽  
Author(s):  
Amein K. Al-Ali ◽  
Ahmed Alsulaiman ◽  
Alhusain J. Alzahrani ◽  
Obeid T. Obeid ◽  
Chitti Babu Vatte ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Sickle Cell Disease ◽  
Sickle Cell ◽  
Cell Disease ◽  
Eastern Province
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