scholarly journals Discovery of potential protein biomarkers associated with sugarcane white leaf disease susceptibility using a comparative proteomic approach

PeerJ ◽  
2022 ◽  
Vol 10 ◽  
pp. e12740
Author(s):  
Kantinan Leetanasaksakul ◽  
Sittiruk Roytrakul ◽  
Narumon Phaonakrop ◽  
Suthathip Kittisenachai ◽  
Siriwan Thaisakun ◽  
...  
Keyword(s):  
Metal Ion ◽  
Defense Mechanism ◽  
Potential Candidate ◽  
Protein Biomarkers ◽  
Sugarcane Varieties ◽  
Leaf Disease ◽  
Proteomic Approach ◽  
White Leaf ◽  
Sugarcane White Leaf ◽  
White Leaf Disease

Sugarcane white leaf disease (SCWLD) is caused by phytoplasma, a serious sugarcane phytoplasma pathogen, which causes significant decreases in crop yield and sugar quality. The identification of proteins involved in the defense mechanism against SCWLD phytoplasma may help towards the development of varieties resistant to SCWLD. We investigated the proteomes of four sugarcane varieties with different levels of susceptibility to SCWLD phytoplasma infection, namely K88-92 and K95-84 (high), KK3 (moderate), and UT1 (low) by quantitative label-free nano-liquid chromatography-tandem mass spectrometry (nano LC-MS/MS). A total of 248 proteins were identified and compared among the four sugarcane varieties. Two potential candidate protein biomarkers for reduced susceptibility to SCWLD phytoplasma were identified as proteins detected only in UT1. The functions of these proteins are associated with protein folding, metal ion binding, and oxidoreductase. The candidate biomarkers could be useful for further study of the sugarcane defense mechanism against SCWLD phytoplasma, and in molecular and conventional breeding strategies for variety improvement.

scholarly journals Candidates for Symbiotic Control of Sugarcane White Leaf Disease

2012 ◽  
Vol 78 (19) ◽  
pp. 6804-6811 ◽  
Author(s):  
Jureemart Wangkeeree ◽  
Thomas A. Miller ◽  
Yupa Hanboonsong
Keyword(s):  
Developmental Stages ◽  
Cloning And Sequencing ◽  
Content Type ◽  
Leaf Disease ◽  
White Leaf ◽  
Symbiotic Control ◽  
Sugarcane White Leaf ◽  
White Leaf Disease ◽  
Bacterial Genes

ABSTRACTThe leafhopperMatsumuratettix hiroglyphicus(Matsumura) is the most important vector of a phytoplasma pathogen causing sugarcane white leaf (SCWL) disease. The purpose of this study was to evaluate candidate bacterial symbionts for possible use as vehicles in the control of the disease. 16S rRNA bacterial genes were amplified from whole bodies ofM. hiroglyphicusleafhoppers and analyzed by cloning and sequencing. Two dominant groups were found: one belonged to theBetaproteobacteriathat did not closely match any sequences in the database and was named bacterium associated withM. hiroglyphicus(BAMH). Another one found to be abundant in this leafhopper is “CandidatusSulcia muelleri” in the orderBacteroidetes, which was previously reported in the insect members of the Auchenorrhyncha. MostM. hiroglyphicusleafhoppers carry both BAMH and “Ca. Sulcia muelleri.” Fluorescentin situhybridization showed that BAMH and “Ca. Sulcia muelleri” colocalized in the same bacteriomes. BAMH was present in the midgut and ovaries of the leafhopper and was found in all developmental stages, including eggs, nymphs, and adults. Because BAMH appears to be specific for the SCWL vector, we evaluated it as a candidate for symbiotic control of sugarcane white leaf disease.

Characterisation of phytoplasma associated with sugarcane white leaf disease in Uttar Pradesh, India

2021 ◽  
pp. 1-9
Author(s):  
Nishtha Mishra ◽  
Surya Prakash Dwivedi ◽  
Ajay Kumar Tiwari ◽  
Malyaj R. Prajapati ◽  
Jitender Singh
Keyword(s):  
Uttar Pradesh ◽  
Leaf Disease ◽  
White Leaf ◽  
Sugarcane White Leaf ◽  
White Leaf Disease

Effect of leaf lamina colour on the behavioral characteristics of Deltocephalus menoni (Hemiptera: Cicadellidae); A vector of sugarcane white leaf disease

Agrica ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 31
Author(s):  
K.M.G. Chanchala ◽  
S.H. Kiriella ◽  
V.K.A.S.M. Wanasinghe ◽  
K.S. Hemachandra ◽  
L. Nugaliyadde ◽  
...  
Keyword(s):  
Behavioral Characteristics ◽  
Leaf Lamina ◽  
Leaf Disease ◽  
White Leaf ◽  
Sugarcane White Leaf ◽  
White Leaf Disease

scholarly journals The role of salicylic acid and benzothiadiazole in decreasing phytoplasma titer of sugarcane white leaf disease

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Manuwat Tintarasara Na Ratchaseema ◽  
Lawan Kladsuwan ◽  
Laurent Soulard ◽  
Pratchya Swangmaneecharern ◽  
Prapat Punpee ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Phenolic Compounds ◽  
Systemic Acquired Resistance ◽  
Acquired Resistance ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Leaf Disease ◽  
White Leaf ◽  
Sugarcane White Leaf ◽  
White Leaf Disease

AbstractThe objective of this research was to study the effect of Benzothiadiazole (BTH) and Salicylic acid (SA) on the systemic acquired resistance (SAR) of sugarcane the phytoplasma associated with the sugarcane white leaf (SCWL) disease. The experiment was conducted on plants of the sugarcane variety Khon Kaen 3 (KK3) infected with SCWL phytoplasma using insect vectors. Biochemical changes related to the SAR such as SA and total phenolic compounds were followed according to 4 different timepoints: 7, 14, 21 and 28 days after inoculation. Together, phytoplasma were quantified by RT-qPCR using the secA gene of phytoplasma. According to our results, the spraying of BTH and SA tended to increase the amounts of SA, total phenolic compounds and a lower presence of phytoplasma in the plants in comparison with the inoculated control. Spraying BTH at a concentration of 2.4 mM and SA at a concentration of 2.4 mM exhibited the best efficiency to reduce the concentration of phytoplasma. According to RT-qPCR results, the inoculated plants sprayed with BTH displayed a significantly lower concentration of phytoplasma compared to the inoculated controls. Overall, our results indicated that the spray of BTH and SA could induce an efficient SAR response to the phytoplasma associated with the SCWL disease. We expect these results will give support to the development of new products for controlling white leaf disease in sugarcane.

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