scholarly journals Characterization of SSR genomic abundance and identification of SSR markers for population genetics in Chinese jujube (Ziziphus jujubaMill.)

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e1735 ◽  
Author(s):  
Peng-cheng Fu ◽  
Yan-Zhao Zhang ◽  
Hui-yuan Ya ◽  
Qing-bo Gao
Keyword(s):  
Ssr Markers ◽  
Chinese Jujube ◽  
Base Pairs ◽  
Fruit Crop ◽  
Dried Fruit ◽  
Null Allele Frequency ◽  
History Of ◽  
Hardy Weinberg Equilibrium ◽  
Ziziphus Jujuba

Chinese jujube (Ziziphus jujubaMill. [Rhamnaceae]), native to China, is a major dried fruit crop in Asia. Although many simple sequence repeat (SSR) markers are available for phylogenetic analysis of jujube cultivars, few of these are validated on the level of jujube populations. In this study, we first examined the abundance of jujube SSRs with repeated unit lengths of 1–6 base pairs, and compared their distribution with those inArabidopsis thaliana. We identified 280,596 SSRs in the assembled genome of jujube. The density of SSRs in jujube was 872.60 loci/Mb, which was much higher than inA. thaliana(221.78 loci/Mb). (A+ T)-rich repeats were dominant in the jujube genome. We then randomly selected 100 SSRs in the jujube genome with long repeats and used them to successfully design 70 primer pairs. After screening using a series of criteria, a set of 20 fluorescently labeled primer pairs was further selected and screened for polymorphisms among three jujube populations. The average number of alleles per locus was 12.8. Among the three populations, mean observed and expected heterozygosities ranged from 0.858 to 0.967 and 0.578 to 0.844, respectively. After testing in three populations, all SSRs loci were in Hardy-Weinberg equilibrium (HWE) in at least one population. Finally, removing high null allele frequency loci and linked loci, a set of 17 unlinked loci was in HWE. These markers will facilitate the study of jujube genetic structure and help elucidate the evolutionary history of this important fruit crop.

scholarly journals Review on Some Features of the Chinese Jujube (Ziziphus jujuba Mill.)

2021 ◽  
Vol 78 (1) ◽  
pp. 10
Author(s):  
Ioan STOLI ◽  
Florin STĂNICĂ
Keyword(s):  
Climate Changes ◽  
Economic Value ◽  
Great Part ◽  
Fruit Tree ◽  
High Tolerance ◽  
Fruit Crops ◽  
Chinese Jujube ◽  
Advertising Campaign ◽  
History Of ◽  
Ziziphus Jujuba

The Chinese jujube (Ziziphus jujuba Mill.), originating from China, with a history of over 4000 years, is one of the major fruit crops cultivated in China and on important areas in Central Asia, India, Iran, the Middle East etc. The high resistances to drought, salty soils and similar climate to that of China recommend this fruit tree as a valuable crop, especially in the context of climate changes and extended desertification. Chinese jujube has a high tolerance to pests and disease, being suitable for organic farming. The lack of divulgative papers on jujube true nutritional, medicinal and economic value, makes it still unknown for the great part of Romanian and European consumers, intensive advertising campaign is needed. The aim of this review is to highlight the requirements of the Chinese jujube (Ziziphus jujuba Mill.) in regards to the soil, water, climate as well as its high nutritional and medicinal values.

scholarly journals Isolation and Characterization of Polymorphic Microsatellite loci from the Invasive Worm Branchiomma luctuosum (Grube, 1870) (Annelida: Sabellidae)

2018 ◽  
Author(s):  
Flávia Ariany Belato ◽  
Elisa Maria Costa-Paiva ◽  
Paulo Cesar Paiva ◽  
Anderson Vilasboa
Keyword(s):  
Null Alleles ◽  
Anthropogenic Activity ◽  
Brazilian Coast ◽  
Isolation And Characterization ◽  
History Of ◽  
Hardy Weinberg Equilibrium ◽  
Polymorphic Microsatellite ◽  
High Level ◽  
Activity Areas

Introduction of exotic species in new areas through anthropic action is one of the major problems that can affect biodiversity. Branchiomma luctuosum is known for its highly invasive potential and the actual occurrence of species commonly associated with port activity areas is an extra evidence that this anthropogenic activity should not be underestimated. In order to develop suitable molecular markers for future studies on colonization routes and population dynamics of the invading individuals of B. luctuosum, nine highly polymorphic microsatellite loci were isolated and their polymorphism levels were evaluated. These loci showed a range of number of alleles per locus from five to ten and all loci had a high level of genetic diversity, and exhibited significant heterozygote deficiencies probably due to the presence of null alleles. Significant deviations from the Hardy-Weinberg equilibrium were detected at several loci and most of them were related to a heterozygous deficit. Heterozygous deficiency can be expected in this case due to the biology and history of this invasive species, in relation to its recent introduction in Brazilian coast and possible action of multiple introductory events.

Characterization of cAMP as an anti-allergic functional factor in Chinese jujube (Ziziphus jujuba Mill.)

2019 ◽  
Vol 60 ◽  
pp. 103414 ◽  
Author(s):  
Tianyi Jiang ◽  
Feng He ◽  
Shiwen Han ◽  
Cheng Chen ◽  
Yani Zhang ◽  
...  
Keyword(s):  
Chinese Jujube ◽  
Ziziphus Jujuba ◽  
Functional Factor

scholarly journals Isolation and Characterization of Polymorphic Microsatellite loci from the Invasive Worm Branchiomma luctuosum (Grube, 1870) (Annelida: Sabellidae)

2018 ◽  
Author(s):  
Flávia Ariany Belato ◽  
Elisa Costa-Paiva ◽  
Anderson Vilasboa
Keyword(s):  
Null Alleles ◽  
Anthropogenic Activity ◽  
Brazilian Coast ◽  
Isolation And Characterization ◽  
History Of ◽  
Hardy Weinberg Equilibrium ◽  
Polymorphic Microsatellite ◽  
High Level ◽  
Activity Areas

Introduction of exotic species in new areas through anthropic action is one of the major problems that can affect biodiversity. Branchiomma luctuosum is known for its highly invasive potential and the actual occurrence of species commonly associated with port activity areas is an extra evidence that this anthropogenic activity should not be underestimated. In order to develop suitable molecular markers for future studies on colonization routes and population dynamics of the invading individuals of B. luctuosum, nine highly polymorphic microsatellite loci were isolated and their polymorphism levels were evaluated. These loci showed a range of number of alleles per locus from five to ten and all loci had a high level of genetic diversity, and exhibited significant heterozygote deficiencies probably due to the presence of null alleles. Significant deviations from the Hardy-Weinberg equilibrium were detected at several loci and most of them were related to a heterozygous deficit. Heterozygous deficiency can be expected in this case due to the biology and history of this invasive species, in relation to its recent introduction in Brazilian coast and possible action of multiple introductory events.

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