Changes in Non-saponin Fatty Acid Content and Increases in Inhibitory Activities of Collagenase and Elastase by Treatment with Saccharomyces cerevisiae of the Supercritical Fluid Extracted Oil of the Adventitious Roots Culture of Wild Mountain Ginseng

2018 ◽  
Vol 26 (2) ◽  
pp. 170-180
Author(s):  
Chul Joong Kim ◽  
Jae Kwon Shim ◽  
Kyung Cheol Kwon ◽  
Jung Dae Lim ◽  
Seon Kang Choi ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fatty Acid ◽  
Supercritical Fluid ◽  
Acid Content ◽  
Adventitious Roots ◽  
Fatty Acid Content ◽  
Inhibitory Activities

scholarly journals Heterologous Production of Dihomo-γ-Linolenic Acid in Saccharomyces cerevisiae

2007 ◽  
Vol 73 (21) ◽  
pp. 6965-6971 ◽  
Author(s):  
Hisashi Yazawa ◽  
Hitoshi Iwahashi ◽  
Yasushi Kamisaka ◽  
Kazuyoshi Kimura ◽  
Tsunehiro Aki ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fatty Acids ◽  
Fatty Acid ◽  
Linolenic Acid ◽  
Acid Content ◽  
Kluyveromyces Lactis ◽  
Fatty Acid Content ◽  
Fatty Acid Desaturase ◽  
Dry Weight ◽  
Medium Composition

ABSTRACT To make dihomo-γ-linolenic acid (DGLA) (20:3n-6) in Saccharomyces cerevisiae, we introduced Kluyveromyces lactis Δ12 fatty acid desaturase, rat Δ6 fatty acid desaturase, and rat elongase genes. Because Fad2p is able to convert the endogenous oleic acid to linoleic acid, this allowed DGLA biosynthesis without the need to supply exogenous fatty acids on the media. Medium composition, cultivation temperature, and incubation time were examined to improve the yield of DGLA. Fatty acid content was increased by changing the medium from a standard synthetic dropout medium to a nitrogen-limited minimal medium (NSD). Production of DGLA was higher in the cells grown at 15�C than in those grown at 20�C, and no DGLA production was observed in the cells grown at 30�C. In NSD at 15�C, fatty acid content increased up until day 7 and decreased after day 10. When the cells were grown in NSD for 7 days at 15�C, the yield of DGLA reached 2.19 μg/mg of cells (dry weight) and the composition of DGLA to total fatty acids was 2.74%. To our knowledge, this is the first report describing the production of polyunsaturated fatty acids in S. cerevisiae without supplying the exogenous fatty acids.

Fatty acid profile and influence of oxythiamine on fatty acid content in Malassezia pachydermatis, Candida albicans and Saccharomyces cerevisiae

Mycoses ◽  
2011 ◽  
Vol 55 (3) ◽  
pp. e106-e113 ◽  
Author(s):  
Adam Tylicki ◽  
Magdalena Siemieniuk ◽  
Pawel Dobrzyn ◽  
Grazyna Ziolkowska ◽  
Magdalena Nowik ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Candida Albicans ◽  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Acid Content ◽  
Fatty Acid Content ◽  
Malassezia Pachydermatis

Effect of initial inoculum on growth and fatty acid content as an α-glucosidase inhibitor in Colletotrichum sp. TSC13 mycelium that cultures under shake and static conditions

2018 ◽  
Vol 19 (2) ◽  
pp. 49-53
Author(s):  
Nina Artanti ◽  
Leonardus B.S. Kardono ◽  
Sanro Tachibana
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Acid Content ◽  
Fatty Acid Content ◽  
Glucose Consumption ◽  
Static Condition ◽  
Initial Inoculum ◽  
Glucosidase Inhibitor ◽  
Inhibitory Activities ◽  
Static Conditions

Colletotrichum species were reported producing various bioactive compounds that may potentially be used in the development of anti-microbial, antioxidant, anticancer and anti-diabetes drugs. Our previous studies showed that the methanol extracts of mycelia from Colletotrichum sp. TSC13, an endophytic fungus isolated from Taxus sumatrana (Miq.) de Laub., were found to have α-glucosidase inhibitory activities. The aim of present study was to examine the effects of initial inoculum on growth and the content of fatty acids as an α-glucosidase inhibitor in Colletotrichum sp. TSC13 that grew under shake and static condition. The results showed that shake and static conditions had more effect compared to the initial inocculum on growth and glucose consumption in growth and glucose consumption. However in terms of fatty acids contents and α-glucosidase inhibitor activities the initial inoculum showed more effect than shake and static conditions

Effect of light intensity on the ultrastructure of the filamentous cyanobacterium, Anabaena variabilis

1988 ◽  
Vol 46 ◽  
pp. 300-301
Author(s):  
C. S. Bricker ◽  
S. R. Barnum ◽  
B. Huang ◽  
J. G. Jaworskl
Keyword(s):  
Fatty Acid ◽  
Light Intensity ◽  
Acid Content ◽  
Fatty Acid Content ◽  
Anabaena Variabilis ◽  
Chloroplast Envelope ◽  
Major Constituent ◽  
Filamentous Cyanobacterium ◽  
Photosynthetic Membrane ◽  
Effect Of Light

Cyanobacteria are Gram negative prokaryotes that are capable of oxygenic photosynthesis. Although there are many similarities between eukaryotes and cyanobacteria in electron transfer and phosphorylation during photosynthesis, there are two features of the photosynthetic apparatus in cyanobacteria which distinguishes them from plants. Cyanobacteria contain phycobiliproteins organized in phycobilisomes on the surface of photosynthetic membrane. Another difference is in the organization of the photosynthetic membranes. Instead of stacked thylakolds within a chloroplast envelope membrane, as seen In eukaryotes, IntracytopIasmlc membranes generally are arranged in three to six concentric layers. Environmental factors such as temperature, nutrition and light fluency can significantly affect the physiology and morphology of cells. The effect of light Intensity shifts on the ultrastructure of Internal membrane in Anabaena variabilis grown under controlled environmental conditions was examined. Since a major constituent of cyanobacterial thylakolds are lipids, the fatty acid content also was measured and correlated with uItrastructural changes. The regulation of fatty acid synthesis in cyanobacteria ultimately can be studied if the fatty acid content can be manipulated.

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