Authentication of Rubus coreanus and Related Species via DNA Barcoding and HPLC Approaches

Abstract Background The northeastern part of the Qinghai-Tibet Plateau (QTP) presents a high number of plateau loach species. As one of the three major groups of fishes distributed on the QTP, plateau loach has high ecological value. However, the taxonomy and systematics of these fish are still controversial, and a large number of new species have been reported. The reason for this phenomenon is that the degree of morphological variation is low, the phylogenetic information provided by morphological and anatomical features used for species identification is relatively poor, and many cryptic species are observed. Based on the high-density sampling points from the biodiversity hotspots surveyed, this study aims to evaluate the biodiversity of plateau loach in the northeastern part of the QTP and reveal the hidden diversity by comparing morphological species with molecular operational taxonomic units (MOTUs). Results After careful identification and comparison of the morphology and DNA barcoding of 1630 specimens, 22 species were identified, with 20 considered valid local species and two identified as new species that had not been previously described. Based on the combination of morphological and molecular methods, a total of 24 native species were found, two of which were cryptic species: Triplophysa robusta sp1 and Triplophysa minxianensis sp1. Fourteen of the 24 species form clusters of barcodes that allow them to be reliably identified. The remaining cases involved 10 closely related species, including rapidly differentiated species and species that seemed to have experienced incomplete lineage sorting or showed introgressions. Conclusions The results highlight the need to combine traditional taxonomies with molecular methods to correctly identify species, especially closely related species, such as the plateau loach. This study provides a basis for protecting the biodiversity of plateau loach.

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Description of ten tadpoles in the genus Boophis from Madagascar

Zootaxa ◽

10.11646/zootaxa.2694.1.1 ◽

2010 ◽

Vol 2694 (1) ◽

pp. 1 ◽

Cited By ~ 1

Author(s):

TAHIRY RASOLONJATOVO HIOBIARILANTO ◽

ROGER-DANIEL RANDRIANIAINA ◽

JULIAN GLOS ◽

AXEL STRAUß ◽

MIGUEL VENCES

Keyword(s):

Dna Barcoding ◽

Related Species ◽

Lower Number ◽

Sister Species ◽

Morphological Similarity ◽

Additional Species ◽

Closely Related Species ◽

Species Groups ◽

First Time

We provide morphological descriptions of the tadpoles of ten species of Malagasy treefrogs of the genus Boophis (family Mantellidae). Based on individuals determined by DNA barcoding, the larvae of eight species are described for the first time: B. anjanaharibeensis, B. axelmeyeri, B. elenae, B. englaenderi, B. luciae, B. rhodoscelis, B. roseipalmatus, and B. vittatus. For two additional species, B. andreonei and B. microtympanum, we provide descriptions from other localities than the previously known larvae. All tadpoles described herein are stream-adapted, exotrophic, and of a relatively generalized morphology. In general, we found a morphological similarity of larvae belonging to phenetic species groups of Boophis, but more detailed analyses showed several differences between taxa that are known to be sister species or closely related to each other: B. luciae has a much higher number of oral papillae than B. sibilans, B. roseipalmatus has a slightly lower number of papillae and possibly a stronger caudal musculature than B. madagascariensis, and B. elenae has a distinctly lower number of oral papillae and keratodonts in the first upper keratodont row than B. sandrae. This indicates that tadpole characters might, in some cases, provide good taxonomic characters in Boophis, a genus in which adults of closely related species are often morphologically extremely similar.

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Description and DNA barcoding assessment of the new species Deutonura gibbosa (Collembola: Neanuridae: Neanurinae), a common springtail of Alps and Jura

Zootaxa ◽

10.11646/zootaxa.2639.1.6 ◽

2010 ◽

Vol 2639 (1) ◽

pp. 59 ◽

Cited By ~ 9

Author(s):

DAVID PORCO ◽

LOUIS DEHARVENG

Keyword(s):

New Species ◽

Dna Barcoding ◽

Related Species ◽

Closely Related Species ◽

The Alps ◽

A New Species

Deutonura gibbosa, a new species of the phlegraea group, is described. It is characterized by a large elongate uneven tubercle Di on Abd. V, with chaetae Di1 and Di2 shift backward. It has otherwise the same chaetotaxic pattern as the subspecies sylvatica of D. deficiens. Deutonura gibbosa sp. nov. is the most widespread Deutonura in the Alps and southern Jura. Its barcode sequence is provided and compared to those of four other Deutonura of the phlegraea group; it unambiguously differs from them, including from D. deficiens sylvatica, its closest relative according to current taxonomy. This is the most convincing example so far that barcode may help to discriminate taxa among closely related species in Collembola.

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DNA barcoding in closely related species: A case study of Primula L. sect. Proliferae Pax (Primulaceae) in China

Journal of Systematics and Evolution ◽

10.1111/j.1759-6831.2011.00115.x ◽

2011 ◽

Vol 49 (3) ◽

pp. 225-236 ◽

Cited By ~ 26

Author(s):

Hai-Fei YAN ◽

Gang HAO ◽

Chi-Ming HU ◽

Xue-Jun GE

Keyword(s):

Dna Barcoding ◽

Related Species ◽

Closely Related Species

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DNA Barcoding Reveals Cryptic Diversity in the Genus Triplophysa (Cypriniformes: Cobitidae, Nemacheilinae) from the Northeastern Qinghai-Tibet Plateau

10.21203/rs.3.rs-42121/v1 ◽

2020 ◽

Author(s):

tai wang ◽

Yanping Zhang ◽

Zhuoyu Yang ◽

Zhe Liu ◽

Yanyan Du

Keyword(s):

New Species ◽

Cryptic Species ◽

Dna Barcoding ◽

Related Species ◽

Native Species ◽

Molecular Methods ◽

Tibet Plateau ◽

Ecological Value ◽

Closely Related Species ◽

Qinghai Tibet Plateau

Abstract Background: The northeastern part of the Qinghai-Tibet Plateau (QTP) is one of the areas where the number of species of plateau loach is the largest. As one of the three major groups of fishes distributed on the QTP, plateau loach have very important ecological value. However, their taxonomy and systematics are still controversial, and a large number of new species have been reported. The reason for this phenomenon is that the degree of morphological variation is low, the phylogenetic information provided by morphological and anatomical features used for species identification is relatively poor, and there are many cryptic species. Based on the high-density sampling points from the biodiversity hotspots surveyed, this study aims to evaluate the taxonomic characteristics of the plateau loach by means of morphology, DNA barcoding and multiple species demarcation methods to accurately describe species and allocate taxonomic units to unknown specimens. Results: After careful identification and comparison of the morphology and DNA barcoding of 1,630 specimens, 22 species were identified, 20 of which were considered valid local species and two of which were new species that had not been described. Based on the combination of morphological and molecular methods, a total of 24 native species have been found, two of which are cryptic species: Triplophysa robusta sp1 and Triplophysa minxianensis sp1. Fourteen of the 24 species form clusters of barcodes, which allow them to be reliably identified. The remaining cases involved 10 closely related species, some of which were rapidly differentiated, had a disputed taxonomic status, or showed introgressions.Conclusions: The results highlight the need to combine traditional taxonomies with molecular methods to correctly identify species, especially in closely related species such as the plateau loach. This study provides a basis for protecting the biodiversity of plateau loach.

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First records raise questions: DNA barcoding of Odonata in the middle of the Mediterranean

Genome ◽

10.1139/gen-2019-0226 ◽

2020 ◽

pp. 1-11 ◽

Cited By ~ 1

Author(s):

Tomasz Rewicz ◽

Arnold Móra ◽

Grzegorz Tończyk ◽

Ada Szymczak ◽

Michal Grabowski ◽

...

Keyword(s):

Dna Barcoding ◽

Related Species ◽

Dna Barcode ◽

Taxonomic Status ◽

Index Number ◽

Reference Library ◽

Nuclear Markers ◽

Closely Related Species ◽

Maltese Islands ◽

Morphological Species

We present the results of the first-ever DNA barcoding study of odonates from the Maltese Islands. In total, 10 morphologically identified species were collected during a two-week long expedition in 2018. Eighty cytochrome c oxidase subunit I (COI) barcodes were obtained from the collected specimens. Intra- and interspecific distances ranged from 0.00% to 2.24% and 0.48% to 17.62%, respectively. Successful species identification based on ascribing a single morphological species to a single Barcode Index Number (BIN) was achieved for eight species (80%). In the case of two species, Ischnura genei and Anax parthenope, BINs were shared with other closely related species. The taxonomic status of I. genei is questionable and the phylogenetic relationship between A. imperator/parthenope is not clear. Further studies involving a series of adult specimens collected in a wide spatial range and nuclear markers are necessary to resolve these cases. Therefore, this dataset serves as an initial DNA barcode reference library for Maltese odonates, within a larger project: Aquatic Macroinvertebrates DNA Barcode Library of Malta.

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