scholarly journals DNA Barcoding Reveals Cryptic Diversity in the Genus Triplophysa (Cypriniformes: Cobitidae, Nemacheilinae) from the Northeastern Qinghai-Tibet Plateau

2020 ◽  
Author(s):  
tai wang ◽  
Yanping Zhang ◽  
Zhuoyu Yang ◽  
Zhe Liu ◽  
Yanyan Du
Keyword(s):  
New Species ◽  
Cryptic Species ◽  
Dna Barcoding ◽  
Related Species ◽  
Native Species ◽  
Molecular Methods ◽  
Tibet Plateau ◽  
Ecological Value ◽  
Closely Related Species ◽  
Qinghai Tibet Plateau

Abstract Background: The northeastern part of the Qinghai-Tibet Plateau (QTP) is one of the areas where the number of species of plateau loach is the largest. As one of the three major groups of fishes distributed on the QTP, plateau loach have very important ecological value. However, their taxonomy and systematics are still controversial, and a large number of new species have been reported. The reason for this phenomenon is that the degree of morphological variation is low, the phylogenetic information provided by morphological and anatomical features used for species identification is relatively poor, and there are many cryptic species. Based on the high-density sampling points from the biodiversity hotspots surveyed, this study aims to evaluate the taxonomic characteristics of the plateau loach by means of morphology, DNA barcoding and multiple species demarcation methods to accurately describe species and allocate taxonomic units to unknown specimens. Results: After careful identification and comparison of the morphology and DNA barcoding of 1,630 specimens, 22 species were identified, 20 of which were considered valid local species and two of which were new species that had not been described. Based on the combination of morphological and molecular methods, a total of 24 native species have been found, two of which are cryptic species: Triplophysa robusta sp1 and Triplophysa minxianensis sp1. Fourteen of the 24 species form clusters of barcodes, which allow them to be reliably identified. The remaining cases involved 10 closely related species, some of which were rapidly differentiated, had a disputed taxonomic status, or showed introgressions.Conclusions: The results highlight the need to combine traditional taxonomies with molecular methods to correctly identify species, especially in closely related species such as the plateau loach. This study provides a basis for protecting the biodiversity of plateau loach.

scholarly journals DNA barcoding reveals cryptic diversity in the underestimated genus Triplophysa (Cypriniformes: Cobitidae, Nemacheilinae) from the northeastern Qinghai-Tibet Plateau

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Tai Wang ◽  
Yan-ping Zhang ◽  
Zhuo-yu Yang ◽  
Zhe Liu ◽  
Yan-yan Du
Keyword(s):  
New Species ◽  
Cryptic Species ◽  
Dna Barcoding ◽  
Related Species ◽  
Native Species ◽  
Molecular Methods ◽  
Tibet Plateau ◽  
Closely Related Species ◽  
Northeastern Part ◽  
Qinghai Tibet Plateau

Abstract Background The northeastern part of the Qinghai-Tibet Plateau (QTP) presents a high number of plateau loach species. As one of the three major groups of fishes distributed on the QTP, plateau loach has high ecological value. However, the taxonomy and systematics of these fish are still controversial, and a large number of new species have been reported. The reason for this phenomenon is that the degree of morphological variation is low, the phylogenetic information provided by morphological and anatomical features used for species identification is relatively poor, and many cryptic species are observed. Based on the high-density sampling points from the biodiversity hotspots surveyed, this study aims to evaluate the biodiversity of plateau loach in the northeastern part of the QTP and reveal the hidden diversity by comparing morphological species with molecular operational taxonomic units (MOTUs). Results After careful identification and comparison of the morphology and DNA barcoding of 1630 specimens, 22 species were identified, with 20 considered valid local species and two identified as new species that had not been previously described. Based on the combination of morphological and molecular methods, a total of 24 native species were found, two of which were cryptic species: Triplophysa robusta sp1 and Triplophysa minxianensis sp1. Fourteen of the 24 species form clusters of barcodes that allow them to be reliably identified. The remaining cases involved 10 closely related species, including rapidly differentiated species and species that seemed to have experienced incomplete lineage sorting or showed introgressions. Conclusions The results highlight the need to combine traditional taxonomies with molecular methods to correctly identify species, especially closely related species, such as the plateau loach. This study provides a basis for protecting the biodiversity of plateau loach.

scholarly journals DNA Barcoding Reveals Cryptic Diversity in the Underestimated Genus Triplophysa (Cypriniformes: Cobitidae, Nemacheilinae) from the Northeastern Qinghai-Tibet Plateau

2020 ◽  
Author(s):  
tai wang ◽  
Yanping Zhang ◽  
Zhuoyu Yang ◽  
Zhe Liu ◽  
Yanyan Du
Keyword(s):  
New Species ◽  
Cryptic Species ◽  
Dna Barcoding ◽  
Related Species ◽  
Native Species ◽  
Incomplete Lineage Sorting ◽  
Molecular Methods ◽  
Tibet Plateau ◽  
Closely Related Species ◽  
Qinghai Tibet Plateau

Abstract Background: The northeastern part of the Qinghai-Tibet Plateau (QTP) is one of the areas where the number of species of plateau loach is the largest. As one of the three major groups of fishes distributed on the QTP, plateau loach have very important ecological value. However, their taxonomy and systematics are still controversial, and a large number of new species have been reported. The reason for this phenomenon is that the degree of morphological variation is low, the phylogenetic information provided by morphological and anatomical features used for species identification is relatively poor, and there are many cryptic species. Based on the high-density sampling points from the biodiversity hotspots surveyed, this study aims to evaluate the biodiversity of plateau loach in the northeastern of QTP and reveal the hidden diversity by comparing morphologic species with molecular operational taxonomic units (MOTUs). Results: After careful identification and comparison of the morphology and DNA barcoding of 1,630 specimens, 22 species were identified, 20 of which were considered valid local species and two of which were new species that had not been described. Based on the combination of morphological and molecular methods, a total of 24 native species have been found, two of which are cryptic species: Triplophysa robusta sp1 and Triplophysa minxianensis sp1. Fourteen of the 24 species form clusters of barcodes, which allow them to be reliably identified. The remaining cases involved 10 closely related species, some of which were rapidly differentiated, seemed to have experienced incomplete lineage sorting, or showed introgressions.Conclusions: The results highlight the need to combine traditional taxonomies with molecular methods to correctly identify species, especially in closely related species such as the plateau loach. This study provides a basis for protecting the biodiversity of plateau loach.

scholarly journals DNA Barcoding Reveals Cryptic Diversity in the Underestimated Genus Triplophysa (Cypriniformes: Cobitidae, Nemacheilinae) from the Northeastern Qinghai-Tibet Plateau

2020 ◽  
Author(s):  
Tai Wang ◽  
Yanping Zhang ◽  
Zhuoyu Yang ◽  
Zhe Liu ◽  
Yanyan Du
Keyword(s):  
New Species ◽  
Cryptic Species ◽  
Dna Barcoding ◽  
Related Species ◽  
Native Species ◽  
Molecular Methods ◽  
Tibet Plateau ◽  
Closely Related Species ◽  
Northeastern Part ◽  
Qinghai Tibet Plateau

Abstract Background: The northeastern part of the Qinghai-Tibet Plateau (QTP) presents a high number of plateau loach species. As one of the three major groups of fishes distributed on the QTP, plateau loach has high ecological value. However, the taxonomy and systematics of these fish are still controversial, and a large number of new species have been reported. The reason for this phenomenon is that the degree of morphological variation is low, the phylogenetic information provided by morphological and anatomical features used for species identification is relatively poor, and many cryptic species are observed. Based on the high-density sampling points from the biodiversity hotspots surveyed, this study aims to evaluate the biodiversity of plateau loach in the northeastern part of the QTP and reveal the hidden diversity by comparing morphological species with molecular operational taxonomic units (MOTUs).Results: After careful identification and comparison of the morphology and DNA barcoding of 1,630 specimens, 22 species were identified, with 20 considered valid local species and two identified as new species that had not been previously described. Based on the combination of morphological and molecular methods, a total of 24 native species were found, two of which were cryptic species: Triplophysa robusta sp1 and Triplophysa minxianensis sp1. Fourteen of the 24 species form clusters of barcodes that allow them to be reliably identified. The remaining cases involved 10 closely related species, including rapidly differentiated species and species that seemed to have experienced incomplete lineage sorting or showed introgressions.Conclusions: The results highlight the need to combine traditional taxonomies with molecular methods to correctly identify species, especially closely related species, such as the plateau loach. This study provides a basis for protecting the biodiversity of plateau loach.

scholarly journals DNA Barcoding Reveals Cryptic Diversity in the Underestimated Genus Triplophysa (Cypriniformes: Cobitidae, Nemacheilinae) from the Northeastern Qinghai-Tibet Plateau

2020 ◽  
Author(s):  
tai wang ◽  
Yanping Zhang ◽  
Zhuoyu Yang ◽  
Zhe Liu ◽  
Yanyan Du
Keyword(s):  
New Species ◽  
Cryptic Species ◽  
Dna Barcoding ◽  
Related Species ◽  
Native Species ◽  
Molecular Methods ◽  
Tibet Plateau ◽  
Closely Related Species ◽  
Northeastern Part ◽  
Qinghai Tibet Plateau

Abstract Background: The northeastern part of the Qinghai-Tibet Plateau (QTP) presents a high number of plateau loach species. As one of the three major groups of fishes distributed on the QTP, plateau loach has high ecological value. However, the taxonomy and systematics of these fish are still controversial, and a large number of new species have been reported. The reason for this phenomenon is that the degree of morphological variation is low, the phylogenetic information provided by morphological and anatomical features used for species identification is relatively poor, and many cryptic species are observed. Based on the high-density sampling points from the biodiversity hotspots surveyed, this study aims to evaluate the biodiversity of plateau loach in the northeastern part of the QTP and reveal the hidden diversity by comparing morphological species with molecular operational taxonomic units (MOTUs). Results: After careful identification and comparison of the morphology and DNA barcoding of 1,630 specimens, 22 species were identified, with 20 considered valid local species and two identified as new species that had not been previously described. Based on the combination of morphological and molecular methods, a total of 24 native species were found, two of which were cryptic species: Triplophysa robusta sp1 and Triplophysa minxianensis sp1. Fourteen of the 24 species form clusters of barcodes that allow them to be reliably identified. The remaining cases involved 10 closely related species, including rapidly differentiated species and species that seemed to have experienced incomplete lineage sorting or showed introgressions.Conclusions: The results highlight the need to combine traditional taxonomies with molecular methods to correctly identify species, especially closely related species, such as the plateau loach. This study provides a basis for protecting the biodiversity of plateau loach.

Description and DNA barcoding assessment of the new species Deutonura gibbosa (Collembola: Neanuridae: Neanurinae), a common springtail of Alps and Jura

Zootaxa ◽  
2010 ◽  
Vol 2639 (1) ◽  
pp. 59 ◽  
Author(s):  
DAVID PORCO ◽  
LOUIS DEHARVENG
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Related Species ◽  
Closely Related Species ◽  
The Alps ◽  
A New Species

Deutonura gibbosa, a new species of the phlegraea group, is described. It is characterized by a large elongate uneven tubercle Di on Abd. V, with chaetae Di1 and Di2 shift backward. It has otherwise the same chaetotaxic pattern as the subspecies sylvatica of D. deficiens. Deutonura gibbosa sp. nov. is the most widespread Deutonura in the Alps and southern Jura. Its barcode sequence is provided and compared to those of four other Deutonura of the phlegraea group; it unambiguously differs from them, including from D. deficiens sylvatica, its closest relative according to current taxonomy. This is the most convincing example so far that barcode may help to discriminate taxa among closely related species in Collembola.

A new cryptic frog species of the Mantidactylus boulengeri group with a divergent vocal sac structure

2002 ◽  
Vol 23 (3) ◽  
pp. 293-304 ◽  
Author(s):  
Miguel Vences ◽  
Frank Glaw
Keyword(s):  
Sexual Selection ◽  
New Species ◽  
Cryptic Species ◽  
Related Species ◽  
Consistent Pattern ◽  
Water Bodies ◽  
Closely Related Species ◽  
Advertisement Calls ◽  
External Structure ◽  
Frog Species

AbstractWe describe a new frog species of the Mantidactylus boulengeri group (Amphibia: Anura: Mantellidae) in the subgenus Gephyromantis from south-eastern Madagascar. It is morphologically similar to M. eiselti and M. thelenae but differs in its advertisement calls with a distinctly shorter note duration, and in its bilobate (not single) subgular vocal sac. Like M. eiselti and M. thelenae the new species Mantidactylus enki is predominantly diurnal and calling males do not aggregate close to water bodies, indicating direct development as it has been demonstrated previously in M. eiselti. The advertisement calls of all three species are described in detail. A comparison with a further pair of cryptic species with bilobate vs. single subgular vocal sac (Mantidactylus tschenki — M. cornutus) did not reveal any consistent pattern of call variation correlated with vocal sac structure. Therefore, sexual selection may also be considered to explain differences in the colour and external structure of vocal sacs among closely related species.

scholarly journals A new black fly species from Brazil, closely related to Simulium guianense Wise (Diptera, Simuliidae), revealed by morphology and DNA barcoding

Zootaxa ◽  
2010 ◽  
Vol 2428 (1) ◽  
pp. 22 ◽  
Author(s):  
NEUSA HAMADA ◽  
MATEUS PEPINELLI ◽  
ALINE MATTOS-GLÓRIA ◽  
SERGIO LUIZ BESSA LUZ
Keyword(s):  
New Species ◽  
Cytochrome Oxidase ◽  
Dna Barcoding ◽  
Related Species ◽  
Cytochrome Oxidase I ◽  
Closely Related Species ◽  
Hydrographic Basin ◽  
Female Pupa ◽  
Gill Filaments ◽  
Nucleotide Divergence

The male, female, pupa and larva of Simulium litobranchium n. sp. are described and illustrated. This new species has 12 gill filaments, as do S. duodenicornium Pepinelli, Hamada & Trivinho-Strixino, S. guianense Wise, S. hirtipupa Lutz, S. perplexum Shelley, Maia-Herzog, Lunas Dias & Couch and S. scutistriatum Lutz, but it can be distinguished from these other species by a combination of characters observed at the pupal and adult stages. DNA barcoding showed that Simulium litobranchium n. sp. exhibits more than 4% nucleotide divergence in cytochrome oxidase I from three other closely related species in the Neotropical subgenus S. (Thyrsopelma). The new species was collected in the Brazilian states of Goiás and Minas Gerais and inhabits rivers 30–40 m in width in the Paraná River hydrographic basin. Females were not observed engaging in anthropophilic behavior.

Description of Rhamma dawkinsi (Lepidoptera: Lycaenidae) a new mountain butterfly from Colombia

Zootaxa ◽  
2017 ◽  
Vol 4338 (3) ◽  
pp. 587 ◽  
Author(s):  
CARLOS PRIETO ◽  
JADWIGA LORENC-BRUDECKA
Keyword(s):  
New Species ◽  
Cryptic Species ◽  
Related Species ◽  
Preliminary Assessment ◽  
Closely Related Species ◽  
A New Species

A preliminary assessment of barcode data for the genus Rhamma suggests the presence of several cryptic species in Colombia. Based on morphology and barcodes, we were able to diagnose one of these lineages as a new species that is described herein as Rhamma dawkinsi, sp. nov. Adult specimens and the genitalia of both sexes are illustrated and compared with R. adunca (Draudt, 1919) and R. comstocki (Johnson, 1992), the most closely related species based on phenotypic appearance. 

scholarly journals Ancyronyx lianlabangorum sp. nov., a new spider riffle beetle from Sarawak, and new distribution records for A. pulcherrimus Kodada, Jäch & Čiampor based on DNA barcodes (Coleoptera, Elmidae)

ZooKeys ◽  
2020 ◽  
Vol 1003 ◽  
pp. 31-55
Author(s):  
Ján Kodada ◽  
Manfred A. Jäch ◽  
Hendrik Freitag ◽  
Zuzana Čiamporová-Zaťovičová ◽  
Katarína Goffová ◽  
...  
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Related Species ◽  
Morphological Characters ◽  
Nucleotide Sequences ◽  
Dna Barcodes ◽  
Closely Related Species ◽  
Diagnostic Characters ◽  
First Time ◽  
New Distribution

Ancyronyx lianlabangorumsp. nov. (Coleoptera, Elmidae), a new spider riffle beetle from the Kelabit Highlands (Sarawak, northern Borneo), is described. Illustrations of the habitus and diagnostic characters of the new species and the similar, polymorphic A. pulcherrimus Kodada et al. are presented. Differences to closely related species, based on COI nucleotide sequences and morphological characters, are discussed. Ancyronyx pulcherrimus is here recorded from Sarawak for the first time, based on DNA barcoding.

A morphometric comparison of three closely related species of Gentiana (Gentianaceae), endemic to the region of the Qinghai–Tibet Plateau

Botany ◽  
2018 ◽  
Vol 96 (3) ◽  
pp. 209-215
Author(s):  
Liang-Hong Ni ◽  
Zhi-Li Zhao
Keyword(s):  
Species Diversity ◽  
Morphometric Analysis ◽  
Related Species ◽  
Tibet Plateau ◽  
Morphological Similarity ◽  
Closely Related Species ◽  
Corolla Length ◽  
Flower Shape ◽  
Qinghai Tibet Plateau ◽  
Gentiana Crassicaulis

Gentiana section Cruciata (Gentianaceae) is mainly found on alpine mountains across Eurasia, with the greatest species diversity occurring on the Qinghai–Tibet Plateau. Gentiana crassicaulis Duthie ex Burk. and the closely related species Gentiana robusta King ex Hook. f. and Gentiana tibetica King ex Hook. f. in sect. Cruciata show extremely high morphological similarity, which could be one of the main reasons for their misidentification. This study targets the authentication of the three closely related species in section Cruciata using morphometric analysis of flower shape. We collected 130 individuals in 14 populations of G. crassicaulis, 38 individuals in 12 populations of G. tibetica, and 45 individuals in 13 populations of G. robusta throughout the respective geographical ranges of the species. We used the parameters for bract length (l1), bract width (w1), inflorescence circumference (c), inflorescence width (w2), calyx length (l2), and corolla length (l3) to quantify the flower shape. The analyses showed that the parameters Rb (l1/w1), Ri (w1/w2), and l3 were significantly different between two of the species: G. crassicaulis and G. tibetica could be identified through corolla length, and although very similar to G. crassicaulis and G. tibetica, G. robusta is distinguishable using the parameters Rb and Ri.

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