scholarly journals Identification and Biotyping Enterotoxigenic Escherichia coli Strains from Tuna Loins and Flakes Produced in Côte d’Ivoire

2018 ◽  
Vol 29 (1) ◽  
pp. 1-7
Author(s):  
Andrée Emmanuelle Sika ◽  
Beugré Léonce Kadji ◽  
Kambire Ollo ◽  
Yolande Aké-Assi ◽  
Rose Koffi-Nevry ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Enterotoxigenic Escherichia Coli ◽  
Cote D’Ivoire ◽  
Côte D'ivoire

scholarly journals Effect of Lactic Acid on Inactivation of Enterotoxigenic Escherichia Coli (ETEC) Isolated from Tuna Loins Produced in Côte D’Ivoire

2020 ◽  
pp. 41-50
Author(s):  
Andree Emmanuelle Sika ◽  
Yolande Ake-Assi ◽  
Therese Anoman ◽  
Ollo Kambire ◽  
Rose Koffi-Nevry ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Liquid Medium ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Enterotoxigenic Escherichia Coli ◽  
Cote D’Ivoire ◽  
E Coli ◽  
Côte D'ivoire ◽  
Study Laboratory

Aims: The aim of this work was to study the effect of lactic acid on the growth of pathogenic strains of Escherichia coli (ETEC) isolated from tuna loins. Study Design: Bacteriological study. Place and Duration of Study: Laboratory of Microbiology of the Central Laboratory of Food hygiene and Agrobusiness (LCHAI), Abidjan, Côte d’Ivoire between September 2014 and December 2014. Methodology: Enterotoxigenic Escherichia coli (ETEC) strains were isolated from tuna loins. Lactic acid (LA) 1%, 2% and 3% were tested in pathogenic strains in liquid medium (brain heart infusion broth, BHI) and in tuna loins. Results: At lactic acid 1%, the bacterial loads decreased during the first two days and then stabilized. E. coli strains in tuna loins were higher (1.25 to 0.9 log CFU/g) than E. coli in liquid medium (0.69 to 0.3 log CFU/g). No bacterial growth was observed in the tuna loins and in BHI for concentrations of 2% and 3% of lactic acid. Conclusion: Lactic acid has an inhibitory effect at 1% and bactericidal effect at 2% and 3% on the growth of E. coli. The use of lactic acid as a preservative could be a solution for the preservation of these products.

scholarly journals Growth and Survival of Enterotoxigenic Escherichia coli (ETEC) Isolated from Tuna Loins Produced in Côte D’ivoire

2020 ◽  
pp. 1-11
Author(s):  
Andrée Emmanuelle Sika ◽  
Lacinan Ouattara ◽  
Dezay Thierry Yoro ◽  
Rose Koffi- Nevry ◽  
Koffi Marcellin Djé
Keyword(s):  
Escherichia Coli ◽  
Liquid Medium ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Enterotoxigenic Escherichia Coli ◽  
Cote D’Ivoire ◽  
Bacteriological Study ◽  
E Coli ◽  
Côte D'ivoire ◽  
Study Laboratory

Aims: The aim of this study was to study the growth of pathogenic strains of Escherichia coli (ETEC) in Tuna loins. Study Design: Bacteriological study. Place and Duration of Study: Laboratory of Microbiology of the Central Laboratory of Food hygiene and Agro-business (LCHAI), Abidjan, Côte d’Ivoire between September 2014 and December 2014. Methodology: Three strains of E. coli (enterotoxigenic strain of E. coli (ETEC), possessing both "elt" and "est" virulence genes resistant to amoxicillin from Tuna loins; E. coli reference strain (ATCC 25992); strain of E. coli (KO 13) from water with the virulence gene "elt") were inoculated in brain heart infusion broth (BHI) and in tuna loins for 120 hours. pH and bacterial loads of E. coli were measured to 0; 3; 6; 12; 24; 48; 72; 96 and 120 hours respectively. Results: The results showed that the three strains of E. coli used in this work survived in liquid medium (BHI) and in Tuna loins even after 5 days (120 hours). The growth curves of these three strains evolved in the same way in liquid medium and in Tuna loins. However, the growth rate of strains of E. coli inoculated in liquid media (BHI) was higher than that of strains inoculated in Tuna loins. Conclusion: Pathogenic strains of E. coli isolated from Tuna loins are a hazard to be considered in the microbiological risk assessment of the consumption of these Tuna products.

scholarly journals Relationship between the Phylogenetic Group of Escherichia coli Strains Isolated in Water and Fish in Fresco Lagoon (Côte d’Ivoire)

2016 ◽  
Vol 5 (10) ◽  
pp. 413-423 ◽  
Author(s):  
Nirupa Nadège Kouadio-Ngbesso ◽  
Nazaire Kouassi ◽  
Kouadio Florent N’guessan ◽  
Ama Adingra ◽  
Bassa Antoine Yobouet ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Phylogenetic Group ◽  
Cote D’Ivoire ◽  
Côte D'ivoire

scholarly journals Antimicrobial resistance of Escherichia coli isolated from irrigation waters in selected urban fields around Yamoussoukro lakes in Côte d'Ivoire

2020 ◽  
Vol 2020 ◽  
Author(s):  
Tchimonbié Messikely Anoman ◽  
Aka Niangoran Marie Stéphanie Kouadio ◽  
Dabé Doga ◽  
Konan Samuel Ahoudjo ◽  
Adolphe Zeze
Keyword(s):  
Escherichia Coli ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Bacterial Resistance ◽  
Chemical Parameters ◽  
Cote D’Ivoire ◽  
E Coli ◽  
Lake System ◽  
Côte D'ivoire ◽  
Physico Chemical

This study examined the distribution of Escherichia coli (E. coli) resistant to eleven antibiotics and possible relationships among physico-chemical parameters and these resistances. It was carried out over two years during four climatic seasons in five lakes of the Yamoussoukro lake system in Côte d'Ivoire. The physicochemical parameters of the lakes studied are temperature, pH, dissolved oxygen (DO), dissolved organic carbon (DOC), turbidity, nitrates, orthophosphates and ammoniacal nitrogen. A total of 744 water samples were collected from five (5) lakes belonging to the lake system, over a two-year period, during four dry and four wet seasons. Escherichia coli loadings were evaluated after isolation on specific chromogenic medium COMPASS ECC Agar and the susceptibility to antibiotics by the agar diffusion method. Physico-chemical parameters were evaluated according to international standards. The physico-chemical results showed that the nutrient contents evolved generally from upstream to downstream of the lake system, independently of the sources of pollution of five lakes of system. However, the lake B, in upstream of the lake system, showed levels of nitrate, orthophosphate and ammoniacal nitrogen often similar to the downstream lakes. This demonstrates a source of chemical pollution in this lake, which flows to lakes D and E, which are themselves subject to other pollution. Bacterial loads and bacterial resistance to antibiotics have strictly evolved from upstream to downstream of the system. Most isolates were resistant to ampicillin (16.46%), tetracycline (12.87%), ciprofloxacin (12.86%) and sulfamethoxazole (10.14%). Antibiotic resistance patterns of E. coli isolates were similar in both years studied, but higher during rainy seasons (GRS and SRS). The spearman rank correlations and the principal component analysis (PCA) revealed significant correlations between bacterial resistance to antibiotics in lakes and water physico-chemical parameters. Turbidity and DOC can help to determine the main resistances in the lake D. Orthophosphates can help to understand the resistance of chloramphenicol and the nitrates can help to understand the resistance of ampicillin in the lake B. Resistance in the lakes A, B and C can be influenced by dissolved oxygen. Overall, this study provided baseline information on bacterial resistance in the lakes of Yamoussoukro in Côte d'Ivoire, showing that these lakes could be reservoirs of antibiotic resistance, potentially dangerous to public health.

scholarly journals Complete Genome Sequence of Ebrios, a Novel T7virus Isolated from the Ebrie Lagoon in Abidjan, Côte d’Ivoire

2018 ◽  
Vol 6 (25) ◽  
Author(s):  
Solange Ngazoa-Kakou ◽  
Cécile Philippe ◽  
Denise M. Tremblay ◽  
Stéphanie Loignon ◽  
Aristide Koudou ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cote D'ivoire ◽  
Complete Genome Sequence ◽  
Stenotrophomonas Maltophilia ◽  
Côte D’Ivoire ◽  
Open Reading Frames ◽  
Cote D’Ivoire ◽  
Content Type ◽  
Côte D'ivoire ◽  
Reading Frames

ABSTRACT The lytic Escherichia coli phage Ebrios was isolated from a water sample collected in Ebrie Lagoon on the Adiopodoumé River in Abidjan (Republic of Côte d’Ivoire, West Africa). The linear genome of this Podoviridae family member contains 39,752 bp, has a G+C content of 52.9%, is composed of 53 open reading frames, and is related to the Stenotrophomonas maltophilia phage IME15.

Molecular Detection of the Arr-2 Gene in Escherichia coli and Klebsiella pneumoniae Resistant to Rifampicin in Abidjan, Côte D'Ivoire

2018 ◽  
Vol 23 (4) ◽  
pp. 1-8 ◽  
Author(s):  
Victoire Gadou ◽  
Nathalie Guessennd ◽  
Abalé Toty ◽  
Fernique Konan ◽  
Mohamed Ouattara ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Molecular Detection ◽  
Cote D’Ivoire ◽  
Côte D'ivoire

scholarly journals Gut microbiota related to Giardia duodenalis, Entamoeba spp. and Blastocystis hominis infections in humans from Côte d’Ivoire

2016 ◽  
Vol 10 (09) ◽  
pp. 1035-1041 ◽  
Author(s):  
Valerio Iebba ◽  
Floriana Santangelo ◽  
Valentina Totino ◽  
Fabrizio Pantanella ◽  
Anatole Monsia ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bacterial Community ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Giardia Duodenalis ◽  
Fecal Microbiota ◽  
Blastocystis Hominis ◽  
Cote D’Ivoire ◽  
Côte D'ivoire ◽  
Microbiota Structure

Introduction: Literature data provide little information about protozoa infections and gut microbiota compositional shifts in humans. This preliminary study aimed to describe the fecal bacterial community composition of people from Côte d’Ivoire harboring Giardia duodenalis, Entamoeba spp., and Blastocystis hominis, in trying to discover possible alterations in their fecal microbiota structure related to the presence of such parasites. Methodology: Twenty fecal samples were collected from people inhabiting three different localities of Côte d’Ivoire for copromicroscopic analysis and molecular identification of G. duodenalis, Entamoeba spp., and B. hominis. Temporal temperature gradient gel electrophoresis (TTGE) was used to obtain a fingerprint of the overall bacterial community; quantitative polymerase chain reaction (qPCR) was used to define the relative abundances of selected bacterial species/group, and multivariate statistical analyses were employed to correlate all data. Results: Cluster analysis revealed a significant separation of TTGE profiles into four clusters (p < 0.0001), with a marked difference for G. duodenalis-positive samples in relation to the others (p = 5.4×10-6). Interestingly, qPCR data showed how G. duodenalis-positive samples were related to a dysbiotic condition that favors potentially harmful species (such as Escherichia coli), while Entamoeba spp./B. hominis-positive subjects were linked to a eubiotic condition, as shown by a significantly higher Faecalibacterium prausnitzii-Escherichia coli ratio. Conclusions: This preliminary investigation demonstrates a differential fecal microbiota structure in subjects infected with G. duodenalis or Entamoeba spp./B. hominis, paving the way for using further next-generation DNA technologies to better understand host-parasite-bacteria interactions, aimed at identifying potential indicators of microbiota changes.

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