Molecular Detection of the Arr-2 Gene in Escherichia coli and Klebsiella pneumoniae Resistant to Rifampicin in Abidjan, Côte D'Ivoire

This study examined the distribution of Escherichia coli (E. coli) resistant to eleven antibiotics and possible relationships among physico-chemical parameters and these resistances. It was carried out over two years during four climatic seasons in five lakes of the Yamoussoukro lake system in Côte d'Ivoire. The physicochemical parameters of the lakes studied are temperature, pH, dissolved oxygen (DO), dissolved organic carbon (DOC), turbidity, nitrates, orthophosphates and ammoniacal nitrogen. A total of 744 water samples were collected from five (5) lakes belonging to the lake system, over a two-year period, during four dry and four wet seasons. Escherichia coli loadings were evaluated after isolation on specific chromogenic medium COMPASS ECC Agar and the susceptibility to antibiotics by the agar diffusion method. Physico-chemical parameters were evaluated according to international standards. The physico-chemical results showed that the nutrient contents evolved generally from upstream to downstream of the lake system, independently of the sources of pollution of five lakes of system. However, the lake B, in upstream of the lake system, showed levels of nitrate, orthophosphate and ammoniacal nitrogen often similar to the downstream lakes. This demonstrates a source of chemical pollution in this lake, which flows to lakes D and E, which are themselves subject to other pollution. Bacterial loads and bacterial resistance to antibiotics have strictly evolved from upstream to downstream of the system. Most isolates were resistant to ampicillin (16.46%), tetracycline (12.87%), ciprofloxacin (12.86%) and sulfamethoxazole (10.14%). Antibiotic resistance patterns of E. coli isolates were similar in both years studied, but higher during rainy seasons (GRS and SRS). The spearman rank correlations and the principal component analysis (PCA) revealed significant correlations between bacterial resistance to antibiotics in lakes and water physico-chemical parameters. Turbidity and DOC can help to determine the main resistances in the lake D. Orthophosphates can help to understand the resistance of chloramphenicol and the nitrates can help to understand the resistance of ampicillin in the lake B. Resistance in the lakes A, B and C can be influenced by dissolved oxygen. Overall, this study provided baseline information on bacterial resistance in the lakes of Yamoussoukro in Côte d'Ivoire, showing that these lakes could be reservoirs of antibiotic resistance, potentially dangerous to public health.

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First Detection of the Plasmid-Mediated Quinolone Resistance Determinants qnrB, qnrS and aac(6’)-Ib-cr in Extended Spectrum Beta-Lactamases-producing Klebsiella pneumonia in Bouaké, Côte d’Ivoire

Microbiology Research Journal International ◽

10.9734/mrji/2020/v30i630232 ◽

2020 ◽

pp. 55-67

Author(s):

Marie N. Tuo ◽

Augustin E. Anoh ◽

Zéphirin O. Wayoro ◽

Baba Coulibaly ◽

Pacome Monemo ◽

...

Keyword(s):

Klebsiella Pneumoniae ◽

Teaching Hospital ◽

Cote D'ivoire ◽

Côte D’Ivoire ◽

Quinolone Resistance ◽

Cote D’Ivoire ◽

Beta Lactamases ◽

Extended Spectrum ◽

Côte D'ivoire ◽

Extended Spectrum Beta Lactamases

Aims: The aims of the present study were to investigate the presence of Plasmid-Mediated Quinolone Resistance (PMQR) determinants and the association of these determinants with Extended Spectrum Beta-Lactamases (ESBLs) genes in ESBL-producing Klebsiella pneumoniae isolates from Teaching Hospital of Bouaké, Côte d’Ivoire. Study Design: It is a retrospective study. Place of Study: Bacteriology-Virology Laboratory of Teaching Hospital, Bouaké, Côte d'Ivoire. Methodology: From January 2015 to December 2016, 96 ESBL-producing Klebsiella pneumoniae isolates were collected from several specimens. Antimicrobial susceptibility of isolates was tested using the standard disk-diffusion method on Mueller-Hinton and interpretation according to recommendations of the 2017 EUCAST. These isolates analyzed for the detection of ESBL (blaCTX-M, blaTEM and blaSHV) and PMQR genes (aac(6’)-Ib-cr, qnrB and qnrS) using simplex PCR. Results: Of the 96 ESBL-producing strains, 85 (88.55%) harbored at least one of the ESBL genes tested. Out of the 85 strains encoding ESBL genes, 96.47% carried blaCTX-M and 92.94% blaSHV and blaTEM genes. Eighty nine (89.6%) of the 96 ESBL producing-isolates were resistant to ciprofloxacin and 84.4% to norfloxacin. Among the 96 strains, 80 (83.33%) were found harboring at least one PMQR gene consisting of 78 (81.3%) aac(6’)-Ib-cr, 61 (63.5%) qnrB and 15 (15.6%) qnrS. Among the PMQR-positive strains, 68.4% coharbored qnrB+acc(6’)-Ib-cr genes, 10.5% qnrB+qnrS+acc(6’)-Ib-cr and 6.6% qnrS+acc(6’)-Ib-cr. The qnrB gene was always linked to aac(6’)-Ib-cr gene. Aac(6’)-Ib-cr gene showed the highest association with three ESBL genes (87.6%), followed by qnrB gene (70.6%), then qnrS (17.7%). Conclusion: The PMQR genes were highly prevalent in ESBL-producing Klebsiella pneumoniae, primarily the aac(6’)-Ib-cr gene. The high associated was observed between ESBL and PMQR genes, notably with the aac(6’)-Ib-cr gene.

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