Preliminary Phytochemical Screening on the Glycosides of Leaf Extracts of Medicinally Important Plant Holoptelea integrifolia (roxb.) Planch Using High Performance Thin Layer Chromatography

2021 ◽  
pp. 27-34
Author(s):  
Ravindra C. Sutar
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Phytochemical Screening ◽  
Leaf Extracts ◽  
Layer Chromatography

scholarly journals PHYTOCHEMICAL STUDIES AND HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY ANALYSIS OF CALAMUS ROTANG LINN LEAF EXTRACTS

2018 ◽  
Vol 11 (2) ◽  
pp. 269
Author(s):  
Pallavi Y ◽  
Hemalatha Kpj
Keyword(s):  
Thin Layer ◽  
Gallic Acid ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Leaf Extract ◽  
Research Work ◽  
Phytochemical Screening ◽  
Leaf Extracts ◽  
Layer Chromatography ◽  
Ethanolic Leaf Extract

 Objective: The present study was aimed at phytochemical screening, quantification, and high-performance thin-layer chromatography (HPTLC) analysis of hexane, chloroform and ethanol leaf extracts of Calamus rotang.Methods: Leaf extracts were prepared according to the polarity of the solvents, i.e., hexane, chloroform, and ethanol. Preliminary phytochemical screening involved the qualitative methods to detect the presence of alkaloids, phenols, flavonoids, saponins, steroids, etc. Quantitative estimation of alkaloids using boldine as standard, phenols using gallic acid as standard, and flavonoids using quercetin as standard were done. HPTLC analysis was done with all three extracts along with quercetin and rutin standards using mobile phase for flavonoids, i.e., 90:10 ratio of chloroform and methanol solvents.Results: Phytochemical screening showed the presence of phenols, flavonoids, alkaloids, etc. Hence, quantification was done for these phytochemicals. Alkaloids were present significantly more in hexane leaf extract, i.e., 2.54±0.216mg boldine equivalents/g. Phenols were present significantly more in ethanolic leaf extract, i.e., 49.04±0.364 mg gallic acid equivalents)/g. Flavonoids were present in significant amount in ethanolic leaf extract, i.e., 458.85±5.74 mg quercetin equivalents/g. HPTLC analysis of hexane, chloroform, and ethanolic extracts showed the presence of flavonoids such as quercetin, rutin, and some unknown flavonoid compounds.Conclusion: Ethanolic leaf extract showed a high amount of phenols and flavonoids. Hence, the extract can be further exploited further for in vitro and in vivo research work.

Phytochemical screening and high performance thin layer chromatography finger printing analysis of green hull of Juglans regia (walnut)

2014 ◽  
Vol 2 (3) ◽  
pp. 235 ◽  
Author(s):  
Pardeep Sharma ◽  
Duraisamy Gomathi ◽  
Ganesan Ravikumar ◽  
Manokaran Kalaiselvi ◽  
Uma Chandrasekaran
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Juglans Regia ◽  
Phytochemical Screening ◽  
Finger Printing ◽  
Layer Chromatography

scholarly journals Phytochemical Screening and High-performance Thin-layer Chromatography Quantification of Vitex trifolia Leaves Hydro-alcoholic Extract: Potential Anti-inflammatory Properties

2021 ◽  
pp. 111-121
Author(s):  
Ahmad Tamim Ghafari ◽  
Aisyah Hasyila Jahidin ◽  
Yuslina Zakaria ◽  
Mizaton Hazizul Hasan
Keyword(s):  
Thin Layer ◽  
Gallic Acid ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Phenolic Content ◽  
Alcoholic Extract ◽  
Phytochemical Screening ◽  
Anti Inflammatory ◽  
Layer Chromatography ◽  
Hptlc Method

Aims: The present study aims to evaluate the phytochemical composition of Vitex trifolia (V. trifolia) leaves hydro-alcoholic extract and to report for the first time, its phenolic content using a validated high-performance thin-layer chromatography (HPTLC) method. Study Design:  Qualitative phytochemical analysis and HTLC densitometric quantitative analysis. Place and Duration of Study: The study was carried out at the Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Malaysia, from March 2020 to December 2020. Methodology:  The preliminary phytochemical screening was carried out qualitatively. The HPTLC analysis was performed on glass-backed 60 F254 silica gel plates using a two steps gradient elution method of the mobile phase. In the first step, methanol was used to develop the plates until 40 mm of developing distance, while in the second step, plates were developed with n-hexane:ethyl acetate:acetic acid (20:9:1, v/v/v) until 80 mm of developing distance. Detection and quantification were performed by densitometric analysis at 254 nm. The method was validated as per the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use (ICH) guideline in terms of linearity, precision, accuracy, the limit of detection (LOD), and the limit of quantification (LOQ). Results: The preliminary phytochemical screening of V. trifolia leaves hydro-alcoholic extract showed the presence of alkaloids, flavonoids, phenols, phytosterols, and terpenoids. The developed HPTLC method was proved to be linear, precise, and accurate. The LOD and LOQ of the method were determined to be 2.01 µg/band and 6.08 µg/band, respectively. The total phenolic content of the extract was calculated from the standard gallic acid calibration plot and found to be 136.94 ± 4.02 mg gallic acid equivalent (GAE)/g of dried extract. Conclusion:  This preliminary study revealed that V. trifolia has a considerable amount of phenolic compounds, which can potentially contribute to its anti-inflammatory, antioxidant, and anticancer activities. Further pharmacological investigations are being carried out to support the folkloric claims.

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