scholarly journals Morphological and Molecular Characterization of Endophytic Fungi Associated with Cocoa (Theobroma cacao L.) in India

2020 ◽  
pp. 1-8
Author(s):  
M. Chaithra ◽  
S. Vanitha ◽  
A. Ramanathan ◽  
V. Jegadeeshwari ◽  
V. Rajesh ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Endophytic Fungi ◽  
Tamil Nadu ◽  
Fungal Endophytes ◽  
Molecular Techniques ◽  
Universal Primers ◽  
Malt Extract ◽  
Plant Parts ◽  
Morphological And Molecular Characterization

Aim: To identify cocoa associated endophytic fungi through morphological and molecular techniques. Place and Duration of Study: Department of Plant Pathology, TNAU, Coimbatore, Tamil Nadu from April 2018 to November 2019. Methodology: Endophytic fungal isolates were isolated from different parts of cocoa using Petrini method. Isolated endophytic fungal strains were grown in Malt extract broth and total genomic DNA was isolated and amplified using universal primers ITS1F and ITS4R. Amplified rDNA was visualized and documented. Results: Morphological and molecular characterization of six endophytic fungi revealed that they are from four different taxa viz., Lasiodiplodia pseudotheobromae PAK-7, Arthrinium rasikravindrae P11, Arthrinium rasikravindrae P12, Diaporthe sp. Vef-3, Lasiodiplodia theobromae TN-R-3,  Colletotrichum sp. TN-9-2 belonging to four different families viz., Botryosphaeriaceae, Apiosporaceae, Diaporthaceae and Glomorellaceae under Phylum Ascomycota. Conclusion:  The present study indicates the distribution and diversity of fungal endophytes in different plant parts of the cocoa tree in south India.

scholarly journals Morphological and Molecular Characterization of Vicia faba and Phaseolus vulgaris Seed-born Fungal Endophytes

2016 ◽  
Vol 10 (1) ◽  
pp. 1-16
Author(s):  
K. S. Akutse ◽  
N. K. Maniani ◽  
S. Ekesi ◽  
K. K.M. Fiabo ◽  
J. Van den Be ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Molecular Characterization ◽  
Vicia Faba ◽  
Fungal Endophytes ◽  
Morphological And Molecular Characterization

scholarly journals Morphological and Molecular Characterization of Nothotylenchus medians and N. similis (Nematoda: Anguinidae) from Southern Alberta, Canada

Horticulturae ◽  
2022 ◽  
Vol 8 (1) ◽  
pp. 74
Author(s):  
Maria Munawar ◽  
Atta Ur Rahman ◽  
Pablo Castillo ◽  
Dmytro P. Yevtushenko
Keyword(s):  
Molecular Characterization ◽  
Phylogenetic Analyses ◽  
Original Description ◽  
Distribution Data ◽  
Plant Parts ◽  
Full Diversity ◽  
Canadian Population ◽  
Inventory Survey ◽  
Morphological And Molecular Characterization

The nematode family, Anguinidae, is a diverse group of polyphagous nematodes, generally known as fungal feeders or parasites of aerial plant parts. Here, we present the morphological and molecular characterization of adult females of two Nothotylenchus species, N. medians and N. similis, along with host association and geographical distribution data of the genus. Both species are recorded as new reports from Canada and designated as reference populations for future studies. Morphological or morphometrical variation was not observed in the Canadian population of N. medians and N. similis, in comparison with the original description. Phylogenetic analyses based on 18S and D2–D3 of 28S genes placed both species within Anguinidae. Since the biology of the genus Nothotylenchus has not been rigorously characterized, the habitat and distribution information presented in this study will shed some light on the ecology of these nematodes. Notably, the detection of N. medians and N. similis in our nematode inventory survey indicates that considerable Nothotylenchus diversity is hidden in these soils. Consequently, increased surveys and more in-depth research are needed to explore the full diversity of anguinids inhabiting these cultivated areas.

Characterization of Antibacterial-Producing Endophytic Fungi of Syzygiumpolyanthum Leaves

2020 ◽  
Vol 20 (4) ◽  
pp. 448-454
Author(s):  
Rahmita Burhamzah ◽  
Gemini Alam ◽  
Herlina Rante
Keyword(s):  
Molecular Characterization ◽  
Endophytic Fungi ◽  
Morphological Characterization ◽  
Test Method ◽  
Analysis Method ◽  
Nitrogen Base ◽  
Rdna Genes ◽  
Antibacterial Screening ◽  
Planting Method

Background: Endophytic fungi live in plants’ tissue and can produce the same bioactive compounds as its host plant produces. Syzygiumpolyanthum leaves have known to be one of the antibacterial compound producers. Aim and Objective: This study aimed to characterize morphologically, microscopically, and molecularly the antibacterial-producing endophytic fungi of Syzygiumpolyanthum leaves. Methods: The isolation of endophytic fungi was done by fragment planting method on PDA medium. The antibacterial screening was performed using the antagonistic test as the first screening followed by the disc diffusion test method. The morphological characterization was based on isolate’s mycelia color, growth pattern, margin, and surface texture of the colony, while the microscopic characterization was based on its hyphae characteristics. The molecular characterization of the isolate was done by nitrogen base sequence analysis method on nucleotide constituent of ITS rDNA genes of the isolate. Results: The results found that isolate DF1 has antibacterial activity against E.coli, S.aureus, P.acne, and P.aeruginosa, with the greatest inhibition at 10% concentration of broth fermentation extract on S.aureus with a diameter of inhibition of 13.77 mm. Conclusion: Based on macroscopic, microscopic, and molecular characterization, DF1 isolate is similar to Ceriporialacerate.

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