scholarly journals Amylase Production by Solid State Fermentation of Agro-industrial Wastes Using Bacillus species

2019 ◽  
pp. 408-414 ◽  
Author(s):  
C. N. Obi ◽  
O. Okezie ◽  
A. N. Ezugwu
Keyword(s):  
Bacillus Subtilis ◽  
Solid State ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Industrial Wastes ◽  
Bacillus Species ◽  
Potato Peel ◽  
Amylase Production ◽  
Study Support ◽  
High Production

This study evaluated amylase production by Bacillus species employing the solid state fermentation (SSF) method using five agro-industrial wastes namely corn cobs, potato peel and maize straw, groundnut husk and corn chaff. Five Bacillus species were tested for amylase production abilities and Bacillus subtilis showed the highest amylase production ability after incubation. Corn chaff gave maximum enzyme production (3.25 U/ml) while the least enzyme was recorded on groundnut husk (2.35 U/ml) at 25. Potato peel had maximum enzyme production by Bacillus subtilis (3.05 U/ml) at pH 7.0 while the least enzyme production was from groundnut husk (2.84 U/ml) at pH 4.0.Thus there was an increase in enzyme production with corresponding increase in substrate concentration. The results obtained in this study support the suitability of using agro-industrial wastes as solid state fermentation substrates for high production of amylase. It’s also a means of solving pollution problems thus making solid state fermentation an attractive method.

scholarly journals Comparative Evaluation of Agroindustrial Byproducts for the Production of Alkaline Protease by Wild and Mutant Strains ofBacillus subtilisin Submerged and Solid State Fermentation

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Hamid Mukhtar ◽  
Ikramul Haq
Keyword(s):  
Bacillus Subtilis ◽  
Solid State ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Soybean Meal ◽  
Alkaline Protease ◽  
Enzyme Production ◽  
Enhanced Production ◽  
Industrial Byproducts ◽  
Agroindustrial Byproducts

The present study describes the screening of different agroindustrial byproducts for enhanced production of alkaline protease by a wild and EMS induced mutant strain ofBacillus subtilisIH-72EMS8. During submerged fermentation, different agro-industrial byproducts were tested which include defatted seed meals of rape, guar, sunflower, gluten, cotton, soybean, and gram. In addition to these meals, rice bran, wheat bran, and wheat flour were also evaluated for protease production. Of all the byproducts tested, soybean meal at a concentration of 20 g/L gave maximum production of the enzyme, that is, 5.74  ±  0.26 U/mL from wild and 11.28  ±  0.45 U/mL from mutant strain, during submerged fermentation. Different mesh sizes (coarse, medium, and fine) of the soybean meal were also evaluated, and a finely ground soybean meal (fine mesh) was found to be the best. In addition to the defatted seed meals, their alkali extracts were also tested for the production of alkaline protease byBacillus subtilis, but these were proved nonsignificant for enhanced production of the enzyme. The production of the enzyme was also studied in solid state fermentation, and different agro-industrial byproducts were also evaluated for enzyme production. Wheat bran partially replaced with guar meal was found as the best substrate for maximum enzyme production under solid state fermentation conditions.

scholarly journals Solid state fermentation of amylase production from Bacillus subtilis D19 using agro-residues

2020 ◽  
Vol 32 (2) ◽  
pp. 1555-1561 ◽  
Author(s):  
Taghreed N. Almanaa ◽  
P. Vijayaraghavan ◽  
Naiyf S. Alharbi ◽  
Shine Kadaikunnan ◽  
Jamal M. Khaled ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Solid State ◽  
Solid State Fermentation ◽  
Amylase Production ◽  
Agro Residues

scholarly journals Characterization of Extracellular Dextranase from a Novel HalophilicBacillus subtilis NRC-B233ba Mutagenic Honey Isolate under Solid State Fermentation

2012 ◽  
Vol 9 (3) ◽  
pp. 1494-1510 ◽  
Author(s):  
Mona A. Esawy ◽  
Sara H. Mansour ◽  
Eman F. Ahmed ◽  
Naziha M. Hassanein ◽  
Hesham A. El Enshasy
Keyword(s):  
Bacillus Subtilis ◽  
Solid State ◽  
Solid State Fermentation ◽  
Crude Extract ◽  
Enzyme Production ◽  
Culture Conditions ◽  
Maximum Activity ◽  
Honey Sample ◽  
Uv Mutation ◽  
Sugar Cane Juice

Bacillus subtilis NRC-B233bwas isolated from Libyan honey sample proved to be a potent dextranase producer by applying solid state fermentation and utilizing corn flour as the sole carbon source. The optimized culture conditions for dextranase productions were 37°C, pH 10, 32 h, and 20% (v/w) moisture content. A unique character of this isolate is its ability to produce steady dextranase irrespective to the presence of NaCl in the medium. The addition of 0.175 Mm CrCl3 increased the enzyme production by about 4.5 fold. Further improvement in enzyme production was achieved by simple UV mutation which increased the enzyme production up to about 2842 U/g. The crude extract has been partially purified about 112-fold from crude extract by only two purification steps involving ultra-filtration. The partially purified dextranase showed its maximum activity at pH 9.2 and 70°C. It retained full activity (100%) at 75°C for one hour. Dextranase activity increased about 4 fold in the presence of 10% NaCl. This enzyme showed variable degradation effect on different types of dextran and its derivatives. The treatment of viscous sugar cane juice with the enzyme preparation resulted in clear visual dextran hydrolysis. These results suggest that the dextranase produced byBacillus subtilis NRC-B233bis industrially applicable.

scholarly journals Polygalacturonase production by Aspergillus nomius MR103 in solid state fermentation using Agro-industrial wastes

2019 ◽  
Vol 11 (2) ◽  
pp. 305-310 ◽  
Author(s):  
Ravi Ketipally ◽  
G. Kranthi Kumar ◽  
M. Raghu Ram
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Orange Peel ◽  
Nitrogen Sources ◽  
Industrial Wastes ◽  
Citrus Peel ◽  
Aspergillus Nomius ◽  
Cost Efficient ◽  
Polygalacturonase Production

The present study was aimed at polygalacturonase production from Aspergillus nomius MR103 under solid state fermentation. A total of 57 fungal strains were obtained from mangrove soils collected from Gilakaladindi and Malakayalanka of Krishna District Andhra Pradesh. For the isolation of fungi these Soil samples were serially diluted and plated on pectin agar media plates.  Among them, the isolate which showed maximum polygalacturonase activity was selected for this study. This strain was identified as A. nomius MR 103 by 18S rRNA sequences analysis. Pectin rich agro-industrial wastes like apple peel, citrus peel, orange peel, wheat bran, rice bran and sugarcane bagasse were used as substrates for polygalacturonase production by A. nomius MR 103. This strain was inoculated into the nutrient broth containing agro industrial wastes under solid state fermentation and amount of Polygalacturonase production was estimated. Maximum enzyme production of 4.83 IU/mg was recorded at pH 7.0 and temperature 35?C after 7 days of incubation, when orange peels were used as substrate.  Addition of carbon and nitrogen sources to orange peel media improved the Polygalcturonase production. Sucrose as carbon and peptone as nitrogen sources were proved  to be the best for  maximum production of Polygalcturonase by A. nomius MR 103 on orange peel substrate. Utilization of agro-industrial by-products provided the establishment of a cost-efficient and sustainable process for enzyme production. 

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