scholarly journals Transplacental Transmission of Filarial Infections and Its Impact on Oxidative Stress and Immune Responses during Foetal Life in Bamenda, North West Cameroon

2020 ◽  
pp. 26-38
Author(s):  
Lemnui Benardette ◽  
Oumar Mahamat ◽  
Ntonifor Helen Ngum ◽  
Ngwenyi Annie- Dorette ◽  
Theresia Kuo Akenji
Keyword(s):  
Oxidative Stress ◽  
Immune Response ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Colorimetric Method ◽  
Transplacental Transmission ◽  
Oxidative Stress Status ◽  
Total Oxidative Stress ◽  
Umbilical Cords

Background: Women commonly harbour filarial infections during their childbearing years, raising the possibility that the developing foetus may be exposed to filarial antigens in the uterus and thereby have altered immunity and susceptibility to infection during early childhood. However, there are no concrete proofs to justify the risk of infections in infants born from mothers having filarial infections during pregnancy. Aim: The purpose of this study was to assess the prevalence of microfilariae in umbilical cord blood and respective mothers and to evaluate the relationship between the cord blood filarial infection and the oxidative stress status and concentration of IL-2, IL-10, IL-13, INF-γ and IgG in umbilical cord blood. Methods: This was a nested case–control study of cords and mothers of normal gestational age (>250 days of gestation). A total of 316 pairs of umbilical cords and mothers were examined. The presence of microfilariae was assessed by microscopy in mothers and cords. Oxidative stress status (total oxidative stress and total oxidative defence) and nitric oxide of umbilical cord and mother’s blood were investigated by the colorimetric method. ELISA was carried out for IL-2, IL-10, IL-13, INF-γ in umbilical cord and mother’s blood. Equally, umbilical cords were subjected to ELISA for total IgG. Results: Results obtained showed that microfilariae had a prevalence of 32.9% and 29.7% in umbilical cord blood and women at time of delivery respectively. High levels of total oxidative stress (TOS) with low total oxidative defence (TAD) was found in filarial infected (Mf +ve) umbilical cord and mother’s blood compared to controls or uninfected (Mf -ve) cords and mothers blood. IL-2 was lower in the blood of microfilariae infected cords and mothers, while INF-γ, IL-13 and IL-10 were higher as compared to microfilariae negative cords and mothers. Equally, plasma total IgG concentration was higher in microfilariae positive cords compared to the negative cords and positively correlated with IL-10. Conclusions: There is high frequency of transplacental transmission of microfilariae. Cord blood filarial infections were associated with a high TOS, a protective immune response with low IL-2 and high INF-γ, and a typical Th2 immune response that was associated with higher concentration of immune total IgG regulatory cytokine IL-10 and IL-13 in neonate.

Oxidative stress status in umbilical cord blood from neonates born to mothers with atopic asthma

2013 ◽  
Vol 27 (2) ◽  
pp. 192-196 ◽  
Author(s):  
Yang Ling ◽  
Guo Yin-Shi ◽  
Shan Hong-Wei ◽  
Zhou Yan ◽  
Li Qiang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Atopic Asthma ◽  
Oxidative Stress Status

scholarly journals Associations between piglet umbilical blood hematological criteria, birth order, birth interval, colostrum intake, and piglet survival

2020 ◽  
Vol 98 (10) ◽  
Author(s):  
Kiah M Gourley ◽  
Joel M DeRouchey ◽  
Mike D Tokach ◽  
Steve S Dritz ◽  
Robert D Goodband ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Birth Weight ◽  
Cord Blood ◽  
Birth Order ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Birth Interval ◽  
Blood Ph ◽  
Umbilical Cords ◽  
Piglet Survival

Abstract A total of 656 pigs (623 live-born and 33 stillborn) from 43 sows were used to evaluate hematological criteria at birth and their association with piglet survival. At birth of each piglet, birth time and order within the litter, weight, umbilical cord status (intact or ruptured) and whether the pig was live-born or stillborn were recorded. A 200µL sample of blood from the umbilical cord was collected and immediately analyzed for concentrations of glucose, oxygen partial pressure (pO2), carbon dioxide partial pressure (pCO2), pH, base excess (BE), bicarbonate (HCO3), saturated oxygen (sO2), total carbon dioxide (TCO2), sodium, potassium, ionized calcium (iCa), hematocrit (Hct), and hemoglobin (Hb) on a hand held iStat portable clinical analyzer (iStat Alinity, Abbott Point of Care Inc., Princeton, NJ). Piglets were categorized into quartiles based on birth order and cumulative birth interval (CumBI). Live-born pigs had higher (P < 0.01) umbilical cord blood pH, HCO3, BE, sO2, TCO2, and birth weight compared with stillborn pigs, but lower (P < 0.01) pCO2, K, iCa, and glucose compared with stillborn pigs. Pigs with intact umbilical cords at birth were associated with higher (P < 0.01) blood pH, HCO3, BE, and TCO2 compared with piglets born with a ruptured umbilical cord. Pigs with intact umbilical cords were associated with lower (P < 0.01) Hct and Hb concentrations and born earlier (P < 0.01) in the birth order compared with pigs born with a ruptured umbilical cord. Pigs that did not survive to weaning had lower (P < 0.01) umbilical cord blood pH, HCO3, BE, sO2, TCO2, Na, glucose, and birth weight, and 24 hr weight compared with pigs alive at weaning. Pigs born in the first quartile for CumBI had higher (P <0.05) pH compared with pigs in the other three quartiles. Umbilical cord blood HCO3, BE, and TCO2 decreased (P <0.05) with each change in CumBI quartile from first to last. Blood glucose was lowest (P <0.05) in pigs born before 44 min and highest in pigs born after 164 min. Umbilical cord blood pH, HCO3, BE, TCO2, Na, glucose, Hct, and Hb were positively associated (P <0.001) with colostrum intake, indicating increased blood values resulted in higher colostrum intake. Although a pig may be live-born, their survival to 24 hr and to weaning is reduced when blood pH, HCO3, BE, and sO2 are lower reiterating the importance of management practices that can reduce the birth interval between pigs and the number of pigs experiencing moderate to severe hypoxia.

Umbilical cord blood markers of oxidative stress in pregnancies complicated by preterm prelabor rupture of membranes

2015 ◽  
Vol 29 (12) ◽  
pp. 1900-1910 ◽  
Author(s):  
Ivana Musilova ◽  
Lubomira Tothova ◽  
Ramkumar Menon ◽  
Barbora Vlkova ◽  
Peter Celec ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Markers Of Oxidative Stress ◽  
Prelabor Rupture Of Membranes

Correlation between circulating biomarkers of oxidative stress of maternal and umbilical cord blood at birth

2006 ◽  
Vol 40 (6) ◽  
pp. 565-570 ◽  
Author(s):  
Sandro Argüelles ◽  
Maria José Machado ◽  
Antonio Ayala ◽  
Alberto Machado ◽  
Blas Hervías
Keyword(s):  
Oxidative Stress ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Circulating Biomarkers ◽  
Biomarkers Of Oxidative Stress

Maternal Epilepsy and Umbilical Cord Blood Oxidative Stress Level

2021 ◽  
pp. 1-10
Author(s):  
Kadriye Yakut ◽  
Fatma Doğa Öcal ◽  
Cem Sanhal ◽  
Özgür Öcal ◽  
Yüksel Oğuz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Stress Level ◽  
Umbilical Cord Blood ◽  
Oxidative Stress Level

scholarly journals Preclinical Evaluation of the Immunomodulatory Properties of Cardiac Adipose Tissue Progenitor Cells Using Umbilical Cord Blood Mesenchymal Stem Cells: A Direct Comparative Study

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Isaac Perea-Gil ◽  
Marta Monguió-Tortajada ◽  
Carolina Gálvez-Montón ◽  
Antoni Bayes-Genis ◽  
Francesc E. Borràs ◽  
...  
Keyword(s):  
Stem Cells ◽  
Immune Response ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Progenitor Cells ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Dependent Manner ◽  
Immunomodulatory Properties

Cell-based strategies to regenerate injured myocardial tissue have emerged over the past decade, but the optimum cell type is still under scrutiny. In this context, human adult epicardial fat surrounding the heart has been characterized as a reservoir of mesenchymal-like progenitor cells (cardiac ATDPCs) with potential clinical benefits. However, additional data on the possibility that these cells could trigger a deleterious immune response following implantation are needed. Thus, in the presented study, we took advantage of the well-established low immunogenicity of umbilical cord blood-derived mesenchymal stem cells (UCBMSCs) to comparatively assess the immunomodulatory properties of cardiac ATDPCs in anin vitroallostimulatory assay using allogeneic mature monocyte-derived dendritic cells (MDDCs). Similar to UCBMSCs, increasing amounts of seeded cardiac ATDPCs suppressed the alloproliferation of T cells in a dose-dependent manner. Secretion of proinflammatory cytokines (IL6, TNFα, and IFNγ) was also specifically modulated by the different numbers of cardiac ATDPCs cocultured. In summary, we show that cardiac ATDPCs abrogate T cell alloproliferation upon stimulation with allogeneic mature MDDCs, suggesting that they could further regulate a possible harmful immune responsein vivo. Additionally, UCBMSCs can be considered as valuable tools to preclinically predict the immunogenicity of prospective regenerative cells.

scholarly journals Prooxidant-Antioxidant Balance in Umbilical Cord Blood of Infants with Meconium Stained of Amniotic Fluid

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Mohammad Hassan Arjmand ◽  
Farhat Ahmad Shah ◽  
Masoud Saleh Moghadam ◽  
Fatemeh Tara ◽  
Amin Jalili ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Amniotic Fluid ◽  
Cord Blood ◽  
Neonatal Mortality ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Meconium Aspiration Syndrome ◽  
Meconium Aspiration ◽  
Mortality And Morbidity ◽  
Healthy Infants

Objective. Using a novel assay termed prooxidant-antioxidant balance (PAB) assay to determine prooxidant-antioxidant balance in umbilical cord blood of infants with meconium stained of amniotic fluid (MSAF). Passage of meconium in amniotic fluid is associated with increase of neonatal mortality and morbidity. This complication occurs in about 15% of infants and is more widespread in postterm neonates. About 1.5 percent of neonates with MSAF develop meconium aspiration syndrome.Method. Sera of 29 umbilical cord blood of infants with MSAF and 32 healthy infants (HI) were collected. Both groups had nonsmoker and non-alcoholic mothers with no diseases. The PAB was measured.Result. There was a significant increase of PAB value (32.8±15.9 HK) in umbilical cord blood of infants with MSAF in comparison to HI (24.5±12.6 HK) (P<0.05). There was no significant correlation between PAB value and age of mothers.Conclusion. The increased PAB value in infants with MSAF showed that these infants are exposed to oxidative stress. Further research with larger population is needed to demonstrate the oxidative stress in infants with MSAF.

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