scholarly journals Effects of Thymus schimperi and Moringa stenopetala Leaf Extracts on Lipid Peroxidation and Total Antioxidant Status in Pre-eclampsia Rat Models

2020 ◽  
pp. 1-7
Author(s):  
Kumlachew Mergiaw ◽  
Yoseph A. Mengesha ◽  
Tesfaye Tolessa ◽  
Eyasu Makonnen ◽  
Solomon Genet ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Lipid Peroxide ◽  
Total Antioxidant Status ◽  
Lipid Peroxides ◽  
Therapeutic Effects ◽  
Dependent Manner ◽  
Leaf Extracts ◽  
Moringa Stenopetala ◽  
Rat Models ◽  
Total Antioxidant

Background: Hypertensive disorders of pregnancy (HDP) are common pregnancy complications, with a cumulative incidence of 7%. Pre-eclampsia (PE) is the most common clinical type of HDP and one of the five top leading causes of maternal mortality worldwide. There is imbalance between lipid peroxides and antioxidant system in PE. Established PE is associated with increased concentrations of oxidative stress markers including lipid peroxidation products, and a reduction in antioxidant concentrations. Methods: A case control experimental method was employed on Wistar rats with induced pre-eclampsia using nitric oxide-nitro-L-arginine methyl ester (L-NAME). Lipid peroxide content was estimated according to the method of Ohkawa et al. 1979. Total antioxidant capacity was assayed using colorimetric azinobis 2, 2′3-ethyl-benzothiazoline-6-sulfonate (ABTS) radical cathion decolorization assay. Results: Lipid peroxides of untreated PE rat models were significantly (p<0.01) higher (0.57±0.08 nmol of malondyaldehide (MDA) per gram tissue weight) compared to normal pregnant controls (0.11±0.03 nmol). PE rat models that received aqueous leaf extracts of Thymus schimperi (ALETS) had (0.09±0.01, 0.07±0.002 and 0.02±0.002 nmol) (p<0.05) while, those PE rat models that received aqueous leaf extracts of Moringa stenopetala (ALEMS) had (0.36±0.08, 0.20±0.003 and 0.13±0.02 nmol) (p<0.05) with daily doses of 250 mg/kg, 500 mg/kg and 1000 mg/kg respectively. On the other hand, untreated PE rat models had significantly (p<0.01) lower levels of serum total anti-oxidants (24.5±0.9 μg/ml of ascorbic acid equivalent) compared to normal pregnant controls (28.1±0.4 μg/ml). ALETS or ALEMS treated PE rat models had significantly (p<0.01) higher levels of serum total anti-oxidants in a dose dependent manner compared to untreated PE controls; (27.6±0.3, 29.5±0.3, 31.2±0.4 μg/ml and 29.2±0.3, 29.7±0.3, 30.6±0.4 μg/ml) with daily doses of 250 mg/kg, 500 mg/kg and 1000 mg/kg respectively. ALETS treated PE rat models had significantly (p<0.05) reduced total lipid peroxides compared to ALEMS treated counterparts. Conclusion: ALETS and ALEMS might have significant therapeutic effects against PE syndrome through reducing lipid peroxides and increasing total anti-oxidants.

scholarly journals Protective Effects of Thymus schimperi and Moringa stenopetala Leaf Extracts on Arterial Blood Pressure and Urine Protein Level in Pre-eclampsia Rat Models

2020 ◽  
pp. 31-37
Author(s):  
Kumlachew Mergiaw ◽  
Yoseph A. Mengesha ◽  
Tesfaye Tolessa ◽  
Eyasu Makonnen ◽  
Solomon Genet ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Arterial Blood Pressure ◽  
Hypertensive Disorder ◽  
Dependent Manner ◽  
Leaf Extracts ◽  
Moringa Stenopetala ◽  
Plant Materials ◽  
Rat Models ◽  
Arterial Blood ◽  
Dose Dependent

Background: Pre-eclampsia (PE) is a common hypertensive disorder during pregnancy and one of the leading causes of perinatal mortality and morbidity. There is no curative modern drug to treat PE. Therefore, researches done on traditional medications have paramount importance in discovering therapeutic and /or protective agents from plant materials. In Ethiopia, there are claims that some indigenous medicinal plants such as Thymus and Moringa can have potential protective functions against PE. The aim of this study was to prove the claims. Methods: Experiments were performed to investigate the effects of aqueous leaf extracts of Thymus schimperi (ALETS) and Moringa stenopetala (ALEMS) on PE rat models induced by nitro-L-arginine methyl ester (L-NAME); a nitric oxide synthase inhibitor. A non-invasive tail cuff blood pressure recorder (Model 179 Amplifier, IITC INC, Life Science Instruments, Woodland Hills, California) was used to determine the arterial blood pressure from rat tail. Urine  analysis  to determine protein levels  was  performed  using  a  dipstick,  semi-qualitative  method  as  per  manufacturer’s instructions (CYBO DFI Korea). Results: ALETS treated PE rat models showed significantly reduced mean arterial blood pressure (MAP) in mmHg; 108±3 (P<0.05), 105±1 (P<0.01) and 99±2 (P<0.01); also the same pattern of results were seen in ALEMS treated PE groups with MAP of 106±1 (P<0.05), 103±1 (P<0.05) and 101±1 (P<0.05) at daily doses of 250 mg/kg, 500 mg/kg and 1000 mg/kg, respectively, compared to untreated case group that had 123±3.7 mmHg at gestation day (GD) 19 . Untreated PE rat models showed significant proteinuria (2000±20 mg/L) throughout the gestation period; while PE rat models treated with either ALETS or ALEMS had significantly reduced (p<0.05) proteinuria in dose dependent pattern (150±15 mg/L) at a dose of 1 gm/kg for each plant extract at GD 19. Conclusion: Aqueous leaf extracts of either Thymus schimperi or Moringa stenopetala might reduce and control arterial pressure in PE rat models in a dose dependent manner.  The extracts also could reduce level of proteinuria in the same pattern. Further investigation should, however, be carried out to confirm their uses in prevention and/or treatment of pregnancy-induced hypertension among human cases.

Differential expression of lipid peroxidation and total antioxidant status in Indian patients with cardiovascular and cerebrovascular disease

2014 ◽  
Vol 92 (7) ◽  
pp. 592-597 ◽  
Author(s):  
Seema Bhargava ◽  
Arif Ali ◽  
Mamta Kankra ◽  
Sabari Das ◽  
Anjali Manocha ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Cerebrovascular Disease ◽  
Vascular Disease ◽  
Vascular Remodeling ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Lipid Peroxides ◽  
Vein Thrombosis ◽  
Occlusive Vascular Disease ◽  
Total Antioxidant

Data from studies examining lipid peroxidation as a mechanism involved with hyperhomocysteinemia (HHcy)-induced vascular remodeling in patients with occlusive vascular disease have been contradictory. It has not yet been studied in Indians within the context of atherogenesis. Therefore, we measured the levels of homocysteine (Hcy), malondialdehyde (MDA) as a measure of lipid peroxides (LPOs), and total antioxidant status (TAS) in the serum of 167 patients with occlusive vascular disease [coronary artery disease (CAD) = 43; cerebrovascular disease (CVD) = 82; peripheral vascular disease (PVD) = 42]. Each of these groups was further divided into groups of individuals with or without HHcy. In the case of CAD and CVD, patients with HHcy had significantly higher LPOs than those without HHcy (p = 0.009, 0.001, respectively). TAS was significantly lower in CVD patients with HHcy than in those without (p = 0.014). In patients with CAD or CVD, Hcy directly correlated with LPOs (p = 0.002, 0.001, respectively). Lipid peroxidation is a significant mechanism in HHcy-induced vascular remodeling in CAD and CVD, but not in PVD, probably because it is not relevant in thrombosis (38 of 42 patients of PVD had deep-vein thrombosis). To explain the significantly lower TAS in CVD, we hypothesized that CVD patients present very early with grave symptoms, whereas CAD and PVD occur over a longer period of time. Therefore, when CVD presents, TAS is still overwhelmed by HHcy-induced oxidative stress. Hence, adjuvant therapy with antioxidants would benefit patients with CVD.

Lipid peroxidation and total antioxidant status in patients with breast cancer

2007 ◽  
Vol 25 (4) ◽  
pp. 377-382 ◽  
Author(s):  
Derya Erten Şener ◽  
Aymelek Gönenç ◽  
Melih Akıncı ◽  
Meral Torun
Keyword(s):  
Breast Cancer ◽  
Lipid Peroxidation ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Total Antioxidant

Prevention of lipid peroxidation does not prevent oxidant-induced myocardial contractile dysfunction

1994 ◽  
Vol 267 (6) ◽  
pp. H2371-H2377 ◽  
Author(s):  
Y. Kong ◽  
E. J. Lesnefsky ◽  
J. Ye ◽  
L. D. Horwitz
Keyword(s):  
Lipid Peroxidation ◽  
Coronary Flow ◽  
Systolic Function ◽  
Lipid Peroxide ◽  
Left Ventricular Pressure ◽  
Lipid Peroxides ◽  
Left Ventricular ◽  
Contractile Dysfunction ◽  
Myocardial Contractile ◽  
Generating System

We tested whether, with exposure to an extraneous iron-catalyzed free radical-generating system, prevention of lipid peroxidation with U74006F, a 21-aminosteroid, could also prevent myocardial contractile dysfunction. Rabbits received either U74006F (10 mg/kg iv) or vehicle (V). Thirty minutes later the hearts were excised and perfused by a non-recirculating Langendorff technique. Six U74006F- and six V-treated hearts were exposed for 7.5 min to a .OH-generating system (H2O2 and Fe(2+)-ADP chelate). Myocardial lipid peroxides were measured by glutathione peroxidase-catalyzed oxidation of exogenous glutathione. With exposure to .OH, cytosolic lipid peroxide levels were increased threefold in V-treated hearts, but there was no increase in U74006F-treated hearts. After 30 min of recovery, developed pressure and maximum first derivative of left ventricular pressure were greater in U74006F-treated hearts than in V-treated hearts but were still 50 and 44% of levels in saline hearts, respectively. Coronary flow was markedly reduced after exposure to free radicals and was only slightly less depressed when U74006F was administered. When coronary flow following oxidant exposure was increased by nitroglycerin, U74006F again only modestly improved systolic function. Thus, although U74006F blocked lipid peroxidation, it only slightly improved the ventricular dysfunction caused by .OH. Therefore, factors other than lipid peroxidation play a major role in oxidant-induced myocardial stunning.

Changes in lipid peroxidation and free radical scavengers in the brain of hyper- and hypothyroid aged rats

1995 ◽  
Vol 147 (2) ◽  
pp. 361-365 ◽  
Author(s):  
T Mano ◽  
R Sinohara ◽  
Y Sawai ◽  
N Oda ◽  
Y Nishida ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Lipid Peroxide ◽  
Lipid Peroxides ◽  
Thiobarbituric Acid ◽  
Free Radical Scavengers ◽  
Aged Rats ◽  
Radical Scavengers ◽  
Old Rats ◽  
The Brain

Abstract To determine how lipid peroxides and free radical scavengers are changed in the brain of hyper- or hypothyroid rats, we examined the behavior of lipid peroxide and free radical scavengers in the cerebral cortex of aged (1·5 years old) rats that had been made hyper- or hypothyroid by the administration of thyroxine or methimazol for 4 weeks. Concentrations of catalase, Mn-superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) were increased in hyperthyroid rats compared with euthyroid rats. Concentrations of total SOD, Cu,Zn-SOD and GSH-PX were increased but that of Mn-SOD was decreased in hypothyroid animals. There were no differences among hyperthyroid, hypothyroid and euthyroid rats in the levels of coenzymes 9 or 10. The concentration of lipid peroxides, determined indirectly by the measurement of thiobarbituric acid reactants, was decreased in hyperthyroid rats but not in hypothyroid rats when compared with euthyroid animals. These findings suggest that free radicals and lipid peroxides are scavenged to compensate for the changes induced by hyper- or hypothyroidism. Journal of Endocrinology (1995) 147, 361–365

Sign in / Sign up

Export Citation Format

Share Document