scholarly journals First Detection of the Plasmid-Mediated Quinolone Resistance Determinants qnrB, qnrS and aac(6’)-Ib-cr in Extended Spectrum Beta-Lactamases-producing Klebsiella pneumonia in Bouaké, Côte d’Ivoire

2020 ◽  
pp. 55-67
Author(s):  
Marie N. Tuo ◽  
Augustin E. Anoh ◽  
Zéphirin O. Wayoro ◽  
Baba Coulibaly ◽  
Pacome Monemo ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Teaching Hospital ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Quinolone Resistance ◽  
Cote D’Ivoire ◽  
Beta Lactamases ◽  
Extended Spectrum ◽  
Côte D'ivoire ◽  
Extended Spectrum Beta Lactamases

Aims: The aims of the present study were to investigate the presence of Plasmid-Mediated Quinolone Resistance (PMQR) determinants and the association of these determinants with Extended Spectrum Beta-Lactamases (ESBLs) genes in ESBL-producing Klebsiella pneumoniae isolates from Teaching Hospital of Bouaké, Côte d’Ivoire. Study Design: It is a retrospective study. Place of Study: Bacteriology-Virology Laboratory of Teaching Hospital, Bouaké, Côte d'Ivoire. Methodology: From January 2015 to December 2016, 96 ESBL-producing Klebsiella pneumoniae isolates were collected from several specimens. Antimicrobial susceptibility of isolates was tested using the standard disk-diffusion method on Mueller-Hinton and interpretation according to recommendations of the 2017 EUCAST. These isolates analyzed for the detection of ESBL (blaCTX-M, blaTEM and blaSHV) and PMQR genes (aac(6’)-Ib-cr, qnrB and qnrS) using simplex PCR. Results: Of the 96 ESBL-producing strains, 85 (88.55%) harbored at least one of the ESBL genes tested. Out of the 85 strains encoding ESBL genes, 96.47% carried blaCTX-M and 92.94% blaSHV and blaTEM genes. Eighty nine (89.6%) of the 96 ESBL producing-isolates were resistant to ciprofloxacin and 84.4% to norfloxacin. Among the 96 strains, 80 (83.33%) were found harboring at least one PMQR gene consisting of 78 (81.3%) aac(6’)-Ib-cr, 61 (63.5%) qnrB and 15 (15.6%) qnrS. Among the PMQR-positive strains, 68.4% coharbored qnrB+acc(6’)-Ib-cr genes, 10.5% qnrB+qnrS+acc(6’)-Ib-cr and 6.6% qnrS+acc(6’)-Ib-cr. The qnrB gene was always linked to aac(6’)-Ib-cr gene. Aac(6’)-Ib-cr gene showed the highest association with three ESBL genes (87.6%), followed by qnrB gene (70.6%), then qnrS (17.7%). Conclusion: The PMQR genes were highly prevalent in ESBL-producing Klebsiella pneumoniae, primarily the aac(6’)-Ib-cr gene. The high associated was observed between ESBL and PMQR genes, notably with the aac(6’)-Ib-cr gene.

scholarly journals High prevalence of ESBL-producing Klebsiella pneumoniae in clinical samples from central Côte d’Ivoire

2020 ◽  
Vol 91 ◽  
pp. 207-209 ◽  
Author(s):  
Eloise Müller-Schulte ◽  
Marie Nonfra Tuo ◽  
Chantal Akoua-Koffi ◽  
Frieder Schaumburg ◽  
Sören L. Becker
Keyword(s):  
Klebsiella Pneumoniae ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Clinical Samples ◽  
Cote D’Ivoire ◽  
Côte D'ivoire ◽  
High Prevalence

scholarly journals Caroli Disease: A Case Report Observed at the Cocody Teaching Hospital of Abidjan (Cote D’Ivoire)

2017 ◽  
Vol 07 (01) ◽  
pp. 28-31
Author(s):  
A. Ouattara ◽  
S. Koné ◽  
E. Allah-Kouadio ◽  
D. Soro ◽  
A. W. Ndjitoyap ◽  
...  
Keyword(s):  
Case Report ◽  
Teaching Hospital ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Cote D’Ivoire ◽  
Caroli Disease ◽  
Côte D'ivoire

scholarly journals First Case of Qnr B6 and Qnr B7 Genes in Enterobacteriaceae Producing Extended-Spectrum Beta-Lactamases in Abidjan, Côte D'ivoire

2019 ◽  
pp. 1-6
Author(s):  
Gadou Victoire ◽  
Toty Abalé Anatole ◽  
Konan Fernique ◽  
Tiékoura Konan Bertin ◽  
Ouattara Mohamed Baguy ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Fluoroquinolone Resistance ◽  
Diffusion Method ◽  
Pasteur Institute ◽  
Cote D’Ivoire ◽  
Extended Spectrum ◽  
Côte D'ivoire ◽  
First Case

Aims: The aim of this study was to characterize fluoroquinolone resistance genes in enterobacteriaceae that produce extended-spectrum β-lactamases, isolated in Abidjan. Place and Duration of Study: Pasteur Institute of Côte d'Ivoire and research unit on emerging tropical infectious diseases of Aix-Marseille University from January 2017 at July 2017. Methodology: The study included 90 enterobacteriaceae producing extended-spectrum β-lactamases isolated from biological products from various hospital services in Abidjan. These strains have been pre-identified and stored at the Center for Biological Resources (CeReB) of Pasteur Institute of Côte d'Ivoire. The identification of the strains was confirmed using the mass spectrometry MALDI-TOF (MS) and the antibiotic sensitivity test was performed using Müeller Hinton's agar diffusion method. The fluoroquinolone resistant genes were detected by conventional PCR and then, sequenced. Results: The strains studied were Escherichia coli (44), Klebsiella pneumoniae (31) and Enterobacter cloacae (15). High resistance rates to ceftriaxone (96.7%), cefotaxime (95.6%), aztreonam (95.6%) and cefoxitin (72.2%) were observed in all strains producing broad spectrum β-lactamases. The resistance rate to fluororquinolones represented by ciprofloxacin was 86.7%. The fluoroquinolone resistance genes detected were qnr A (3.3%) and qnr B (42.2%). Sequencing identified the qnr A1 (3.3%), qnr B1 (31.1%), qnr B6 (2.2%) and qnr B7 (1.1%) genes. Conclusion: This study made it possible to identify fluoroquinolone resistance genes in enterobacteriaceae producing β-lactamases which have an extended spectrum in Abidjan.

Oropharyngeal candidiasis prevalence among HIV-infected patients at the teaching hospital of Treichville (Abidjan, Côte d’Ivoire)

2017 ◽  
Vol 27 (4) ◽  
pp. 549-553 ◽  
Author(s):  
A. Konaté ◽  
P.C.M. Barro-Kiki ◽  
K.F. Kassi ◽  
K.E. Angora ◽  
H. Vanga-Bosson ◽  
...  
Keyword(s):  
Teaching Hospital ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Oropharyngeal Candidiasis ◽  
Cote D’Ivoire ◽  
Côte D'ivoire
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