scholarly journals High prevalence of ESBL-producing Klebsiella pneumoniae in clinical samples from central Côte d’Ivoire

2020 ◽  
Vol 91 ◽  
pp. 207-209 ◽  
Author(s):  
Eloise Müller-Schulte ◽  
Marie Nonfra Tuo ◽  
Chantal Akoua-Koffi ◽  
Frieder Schaumburg ◽  
Sören L. Becker
Keyword(s):  
Klebsiella Pneumoniae ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Clinical Samples ◽  
Cote D’Ivoire ◽  
Côte D'ivoire ◽  
High Prevalence

scholarly journals High prevalence of Schistosoma haematobium × Schistosoma bovis hybrids in schoolchildren in Côte d'Ivoire

Parasitology ◽  
2019 ◽  
Vol 147 (3) ◽  
pp. 287-294 ◽  
Author(s):  
Etienne K. Angora ◽  
Jean-François Allienne ◽  
Olivier Rey ◽  
Hervé Menan ◽  
André O. Touré ◽  
...  
Keyword(s):  
Cote D'ivoire ◽  
Schistosoma Haematobium ◽  
Côte D’Ivoire ◽  
Sub Saharan Africa ◽  
Cote D’Ivoire ◽  
Mitochondrial Cox1 ◽  
Fta Cards ◽  
Côte D'ivoire ◽  
High Prevalence ◽  
Sub Saharan

AbstractSchistosomiasis is a neglected tropical disease, though it is highly prevalent in many parts of sub-Saharan Africa. While Schistosoma haematobium-bovis hybrids have been reported in West Africa, no data about Schistosoma hybrids in humans are available from Côte d'Ivoire. This study aimed to identify and quantify S. haematobium-bovis hybrids among schoolchildren in four localities of Côte d'Ivoire. Urine samples were collected and examined by filtration to detect Schistosoma eggs. Eggs were hatched and 503 miracidia were individually collected and stored on Whatman® FTA cards for molecular analysis. Individual miracidia were molecularly characterized by analysis of mitochondrial cox1 and nuclear internal transcribed spacer 2 (ITS 2) DNA regions. A mitochondrial cox1-based diagnostic polymerase chain reaction was performed on 459 miracidia, with 239 (52.1%) exhibiting the typical band for S. haematobium and 220 (47.9%) the S. bovis band. The cox1 and ITS 2 amplicons were Sanger sequenced from 40 randomly selected miracidia to confirm species and hybrids status. Among the 33 cox1 sequences analysed, we identified 15 S. haematobium sequences (45.5%) belonging to seven haplotypes and 18 S. bovis sequences (54.5%) belonging to 12 haplotypes. Of 40 ITS 2 sequences analysed, 31 (77.5%) were assigned to pure S. haematobium, four (10.0%) to pure S. bovis and five (12.5%) to S. haematobium-bovis hybrids. Our findings suggest that S. haematobium-bovis hybrids are common in Côte d'Ivoire. Hence, intense prospection of domestic and wild animals is warranted to determine whether zoonotic transmission occurs.

scholarly journals First Detection of the Plasmid-Mediated Quinolone Resistance Determinants qnrB, qnrS and aac(6’)-Ib-cr in Extended Spectrum Beta-Lactamases-producing Klebsiella pneumonia in Bouaké, Côte d’Ivoire

2020 ◽  
pp. 55-67
Author(s):  
Marie N. Tuo ◽  
Augustin E. Anoh ◽  
Zéphirin O. Wayoro ◽  
Baba Coulibaly ◽  
Pacome Monemo ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Teaching Hospital ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Quinolone Resistance ◽  
Cote D’Ivoire ◽  
Beta Lactamases ◽  
Extended Spectrum ◽  
Côte D'ivoire ◽  
Extended Spectrum Beta Lactamases

Aims: The aims of the present study were to investigate the presence of Plasmid-Mediated Quinolone Resistance (PMQR) determinants and the association of these determinants with Extended Spectrum Beta-Lactamases (ESBLs) genes in ESBL-producing Klebsiella pneumoniae isolates from Teaching Hospital of Bouaké, Côte d’Ivoire. Study Design: It is a retrospective study. Place of Study: Bacteriology-Virology Laboratory of Teaching Hospital, Bouaké, Côte d'Ivoire. Methodology: From January 2015 to December 2016, 96 ESBL-producing Klebsiella pneumoniae isolates were collected from several specimens. Antimicrobial susceptibility of isolates was tested using the standard disk-diffusion method on Mueller-Hinton and interpretation according to recommendations of the 2017 EUCAST. These isolates analyzed for the detection of ESBL (blaCTX-M, blaTEM and blaSHV) and PMQR genes (aac(6’)-Ib-cr, qnrB and qnrS) using simplex PCR. Results: Of the 96 ESBL-producing strains, 85 (88.55%) harbored at least one of the ESBL genes tested. Out of the 85 strains encoding ESBL genes, 96.47% carried blaCTX-M and 92.94% blaSHV and blaTEM genes. Eighty nine (89.6%) of the 96 ESBL producing-isolates were resistant to ciprofloxacin and 84.4% to norfloxacin. Among the 96 strains, 80 (83.33%) were found harboring at least one PMQR gene consisting of 78 (81.3%) aac(6’)-Ib-cr, 61 (63.5%) qnrB and 15 (15.6%) qnrS. Among the PMQR-positive strains, 68.4% coharbored qnrB+acc(6’)-Ib-cr genes, 10.5% qnrB+qnrS+acc(6’)-Ib-cr and 6.6% qnrS+acc(6’)-Ib-cr. The qnrB gene was always linked to aac(6’)-Ib-cr gene. Aac(6’)-Ib-cr gene showed the highest association with three ESBL genes (87.6%), followed by qnrB gene (70.6%), then qnrS (17.7%). Conclusion: The PMQR genes were highly prevalent in ESBL-producing Klebsiella pneumoniae, primarily the aac(6’)-Ib-cr gene. The high associated was observed between ESBL and PMQR genes, notably with the aac(6’)-Ib-cr gene.

scholarly journals High Prevalence of Shared International Type 53 among Mycobacterium tuberculosis Complex Strains in Retreated Patients from Côte d’Ivoire

PLoS ONE ◽  
2012 ◽  
Vol 7 (9) ◽  
pp. e45363 ◽  
Author(s):  
Timothée Ouassa ◽  
Emanuele Borroni ◽  
Guillaume Yao Loukou ◽  
Hortense Faye-Kette ◽  
Jacquemin Kouakou ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Mycobacterium Tuberculosis Complex ◽  
Tuberculosis Complex ◽  
Cote D’Ivoire ◽  
Côte D'ivoire ◽  
High Prevalence

High Prevalence of Strongyloides stercoralis Among School Children in Rural Côte d'Ivoire

2010 ◽  
Vol 96 (2) ◽  
pp. 431-433 ◽  
Author(s):  
Dominik Glinz ◽  
Nicaise A. N'Guessan ◽  
Jürg Utzinger ◽  
Eliézer K. N'Goran
Keyword(s):  
School Children ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Strongyloides Stercoralis ◽  
Cote D’Ivoire ◽  
Côte D'ivoire ◽  
High Prevalence

Molecular Detection of the Arr-2 Gene in Escherichia coli and Klebsiella pneumoniae Resistant to Rifampicin in Abidjan, Côte D'Ivoire

2018 ◽  
Vol 23 (4) ◽  
pp. 1-8 ◽  
Author(s):  
Victoire Gadou ◽  
Nathalie Guessennd ◽  
Abalé Toty ◽  
Fernique Konan ◽  
Mohamed Ouattara ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Molecular Detection ◽  
Cote D’Ivoire ◽  
Côte D'ivoire

scholarly journals Antifungal Resistance Profile for Clinical Isolates of Candida spp. at Institute Pasteur of Côte d’Ivoire

2021 ◽  
Vol 4 (2) ◽  
Author(s):  
Offianan Andre TOURE
Keyword(s):  
Cote D'ivoire ◽  
Candida Species ◽  
Côte D’Ivoire ◽  
Vaginal Swab ◽  
Clinical Samples ◽  
Identification System ◽  
Cote D’Ivoire ◽  
Côte D'ivoire ◽  
Sensitivity Rate ◽  
Susceptibility Patterns

Background: The aim of this study was to identify Candida species isolated from clinical samples and detect their susceptibility patterns to antifungal agents. Methodology: This study was conducted on clinical samples collected from patients with suspected candida infection referring to mycology laboratory of Institute Pasteur of Côte d’Ivoire for diagnosis. Candida species were isolated and identified using conventional and the innovative VITEK 2 microbial identification system. Antifungal susceptibility test for Amphotericin-B, 5-Flucytosine, Fluconazole, Itraconazole and Voriconazole were performed using ATB Fungus 3® of Biomerieux. Results: Most of the isolates were obtained from vaginal swabs 806 (64.8%), followed by Nail scrapings 49 (5.67%). Overall C. albicans counting was 58.9% (732/1234) of the infections. Sensitivity rates of C. albicans species from vaginal swab to 5-FC, AMB, FCZ, VCR and ITR were 97.26, 94.34, 94.89, 95.98 and 88.69 respectively. Resistance rates of C. albicans from vaginal swab were observed with AMB (5.66%) and ITR (5.29%). Sensitivity rate of C. glabrata was 100% with 5-FC, AMB and VCR. All C.krusei species were resistant to fluconazole. The sensitivity rate of C. tropicalis to the antifungal drugs tested varied between 91.66% with ITR to 100 with VCR. Conclusion: C. albicans was the most common of the candida species isolated in this study and remains sensitive to the drugs tested. The study showed resistance of all C. krusei strains to fluconazole. Knowledge about etiologic agents and their susceptibility patterns is helpful for successful treatment of the patients.

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