scholarly journals Isolation, Identification and Production of Biobutanol by Different Clostridium species Isolated from Soil Using Waste Paper and Sugar Cane Molasses

2018 ◽  
pp. 1-9
Author(s):  
G. Owuna ◽  
M. D. Makut ◽  
I. K. Ekeleme ◽  
S. O. Obiekezie
Keyword(s):  
16S Rrna ◽  
Sugar Cane ◽  
Uv Light ◽  
Waste Paper ◽  
Clostridium Species ◽  
Paper Substrate ◽  
Fuel Component ◽  
Cane Molasses ◽  
Induce Mutation ◽  
Microbiological Methods

Butanol as fuel component has some advantages compared to ethanol. The Study was aimed at isolation of different types of Clostridium species from the soil and screening for their ability to produce bio-butanol. Soil samples were collected from three different areas in keffi. Clostridium species were isolated using Reinforce Clostridial media and identified using standard microbiological methods, mutation was induced in the Clostridium isolates and 16s rRNA identification technique was used to identify the isolates. Quantitative screening of the Clostridium species isolates capacity in bio-butanol was carried out using sugar cane molasses and waste paper substrate. The result of 16s rRNA of the Clostridium isolated from old barracks was C. acetobutylicium the one isolated from Angwan kwara was C. perfringenes and C. difficile was isolated from Angwan Jaba. Screening for bio-butanol producing ability of the clostridium isolates, shows that C. acetobutylicium produce highest bio-butanol before been exposed to UV light to induce mutation with 9.61± 0.94 mg/l using sugar cane molasses and 4.89± 0.19 mg/l using waste paper followed by C. perfringenes 6.24± 0.61 mg/l using molasses and 3.21±0.11 mg/l using waste paper. After exposing to UV light to induce mutation C. acetobutylicium produced 18.03 ± 0.17 mg/l using molasses and 8.63± 0.06 mg/l using waste paper substrate follow by C. perfringenes 14.19 ± 0.11 mg/l and 5.99 ± 0.31 mg/l. From this study it was observed that C. acetobutylicium has the capacity to produce bio-butanol and also inducing mutation will enhance the capacity of Clostridium species in the production of bio-butanol.

scholarly journals Effects of Sugar Cane Molasses Addition on the Fermentation Quality, Microbial Community, and Tastes of Alfalfa Silage

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 355
Author(s):  
Runbo Luo ◽  
Yangdong Zhang ◽  
Fengen Wang ◽  
Kaizhen Liu ◽  
Guoxin Huang ◽  
...  
Keyword(s):  
Sugar Cane ◽  
Ph Value ◽  
Electronic Tongue ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Cane Molasses ◽  
Fermentation Quality ◽  
Alfalfa Silage ◽  
Additive Control

The objective was to study the effects of sugar cane molasses addition on the fermentation quality and tastes of alfalfa silage. Fresh alfalfa was ensiled with no additive (Control), 1% molasses (M1), 2% molasses (M2), and 3% molasses (M3) for 206 days. The chemical composition and fermentation characteristics of the alfalfa silages were determined, the microbial communities were described by 16S rRNA sequencing, and the tastes were evaluated using an electronic tongue sensing system. With the amount of added molasses (M), most nutrition (dry matter and crude protein) was preserved and water-soluble carbohydrates (WSC) were sufficiently used to promote the fermentation, resulting in a pH reduction from 5.16 to 4.48. The lactic acid (LA) content and LA/acetic acid (AA) significantly increased, indicating that the fermentation had turned to homofermentation. After ensiling, Enterococcus and Lactobacillus were the dominant genus in all treatments and the undesirable microbes were inhibited, resulting in lower propionic acid (PA), butyric acid (BA), and NH3-N production. In addition, bitterness, astringency, and sourness reflected tastes of alfalfa silage, while umami and sourness changed with the amount of added molasses. Therefore, molasses additive had improved the fermentation quality and tastes of alfalfa silage, and the M3 group obtained the ideal pH value (below 4.5) and the best condition for long-term preservation.

Production of ethanol from sugar cane molasses by Zymomonas mobilis

1982 ◽  
Vol 4 (4) ◽  
pp. 253-256 ◽  
Author(s):  
H. J. J. van Vuuren ◽  
Lourine Meyer
Keyword(s):  
Sugar Cane ◽  
Zymomonas Mobilis ◽  
Cane Molasses

Color Standards for Sugar Cane Sirup and Edible Sugar Cane Molasses

1952 ◽  
Vol 24 (5) ◽  
pp. 832-834 ◽  
Author(s):  
C. B. Broeg ◽  
C. F. Walton
Keyword(s):  
Sugar Cane ◽  
Cane Molasses

Identification and Semiquantitative Estimation of Antibiotics Added to Complete Feeds, Premixes, and Concentrates

1999 ◽  
Vol 82 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Jean-Louis Gafner
Keyword(s):  
Staphylococcus Aureus ◽  
Quality Assurance ◽  
Uv Light ◽  
Acid Extraction ◽  
Antimicrobial Compounds ◽  
Routine Method ◽  
Agar Diffusion ◽  
Microbiological Methods ◽  
Layer Chromatography ◽  
Feed Samples

Abstract Classical microbiological methods for determining antimicrobial compounds in feeds are nonspecific. Thus, there is a need to identify biological activity, and bioautography is used for thispurpose. A routine method for detecting the following antimicrobial sub stances in feeds is described: avilamycin, avoparcin, Zn-bacitracin, erythromycin, flavomycin, furazolidone, lasalocid, monensin, narasin, penicillin, salinomycin, spiramycin, tetracyclines, tylosin, and virginiamycin. Carbadox can be detected by UV light examination of the plates prior to bioautography. Semiquantitative estimations of antibiotic content are compared with quantitative determinations of the above mentioned sub stances in feeds, except erythromycin, penicillin, and tetracyclines. Detection limits range from 0.1 mg/kg (chlortetracycline) to 20 mg/kg (lasalocid). The method involves agar diffusion of buffered samples, a neutral extraction of polyether antibiotics followed bythin-layer chromatography (TLC), and an acid extraction for other antibiotics followed by TLC. Five test bacteria were used for the main detection by agar diffusion: Micrococcus luteusATCC 9341, Staphylococcus aureus ATCC 6538P, Corynebacterium xerosis NCTC 9755, Bacillus cereus ATCC 11778, and B. subtilis ATCC 6633. Identification after TLC was achieved by bioautography with the most sensitive microorganism(s). This method allows one laboratory technician to analyze up to 30 feed samples within 2.5 working days, provided that feeds of the same category are analyzed in the same run, and that labels of additives are available. Qualitative and semiquantitative information are valuable when performing a quantitative antibiotic determination and it provides proof that the activity determined is due to the tested substance. This last feature is essential from the perspective of quality assurance of results.

Strategies for the development of a side stream process for polyhydroxyalkanoate (PHA) production from sugar cane molasses

2007 ◽  
Vol 130 (4) ◽  
pp. 411-421 ◽  
Author(s):  
M.G.E. Albuquerque ◽  
M. Eiroa ◽  
C. Torres ◽  
B.R. Nunes ◽  
M.A.M. Reis
Keyword(s):  
Sugar Cane ◽  
Cane Molasses ◽  
Side Stream ◽  
Pha Production

Use of a packed-bed reactor for anaerobic treatment of stillage of sugar cane molasses

1982 ◽  
Vol 4 (2) ◽  
pp. 127-132 ◽  
Author(s):  
F. S�nchez Riera ◽  
S. Valz-Gianinet ◽  
D. Callieri ◽  
F. Si�eriz
Keyword(s):  
Sugar Cane ◽  
Packed Bed ◽  
Anaerobic Treatment ◽  
Packed Bed Reactor ◽  
Cane Molasses

Production of polyhydroxyalkanoates from fermented sugar cane molasses by a mixed culture enriched in glycogen accumulating organisms

2010 ◽  
Vol 145 (3) ◽  
pp. 253-263 ◽  
Author(s):  
Simon Bengtsson ◽  
Ana R. Pisco ◽  
Maria A.M. Reis ◽  
Paulo C. Lemos
Keyword(s):  
Mixed Culture ◽  
Sugar Cane ◽  
Cane Molasses
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