Is It Speech or Song? Effect of Melody Priming on Pitch Perception of Modified Mandarin Speech

Tonal languages make use of pitch variation for distinguishing lexical semantics, and their melodic richness seems comparable to that of music. The present study investigated a novel priming effect of melody on the pitch processing of Mandarin speech. When a spoken Mandarin utterance is preceded by a musical melody, which mimics the melody of the utterance, the listener is likely to perceive this utterance as song. We used functional magnetic resonance imaging to examine the neural substrates of this speech-to-song transformation. Pitch contours of spoken utterances were modified so that these utterances can be perceived as either speech or song. When modified speech (target) was preceded by a musical melody (prime) that mimics the speech melody, a task of judging the melodic similarity between the target and prime was associated with increased activity in the inferior frontal gyrus (IFG) and superior/middle temporal gyrus (STG/MTG) during target perception. We suggest that the pars triangularis of the right IFG may allocate attentional resources to the multi-modal processing of speech melody, and the STG/MTG may integrate the phonological and musical (melodic) information of this stimulus. These results are discussed in relation to subvocal rehearsal, a speech-to-song illusion, and song perception.

Auditory experience controls the maturation of song discrimination and sexual response in Drosophila

In birds and higher mammals, auditory experience during development is critical to discriminate sound patterns in adulthood. However, the neural and molecular nature of this acquired ability remains elusive. In fruit flies, acoustic perception has been thought to be innate. Here we report, surprisingly, that auditory experience of a species-specific courtship song in developing Drosophila shapes adult song perception and resultant sexual behavior. Preferences in the song-response behaviors of both males and females were tuned by social acoustic exposure during development. We examined the molecular and cellular determinants of this social acoustic learning and found that GABA signaling acting on the GABAA receptor Rdl in the pC1 neurons, the integration node for courtship stimuli, regulated auditory tuning and sexual behavior. These findings demonstrate that maturation of auditory perception in flies is unexpectedly plastic and is acquired socially, providing a model to investigate how song learning regulates mating preference in insects.

Song discrimination by nestling collared flycatchers during early development

Pre-zygotic isolation is often maintained by species-specific signals and preferences. However, in species where signals are learnt, as in songbirds, learning errors can lead to costly hybridization. Song discrimination expressed during early developmental stages may ensure selective learning later in life but can be difficult to demonstrate before behavioural responses are obvious. Here, we use a novel method, measuring changes in metabolic rate, to detect song perception and discrimination in collared flycatcher embryos and nestlings. We found that nestlings as early as 7 days old respond to song with increased metabolic rate, and, by 9 days old, have increased metabolic rate when listening to conspecific when compared with heterospecific song. This early discrimination between songs probably leads to fewer heterospecific matings, and thus higher fitness of collared flycatchers living in sympatry with closely related species.

Central neural circuitry mediating courtship song perception in male Drosophila

Animals use acoustic signals across a variety of social behaviors, particularly courtship. In Drosophila, song is detected by antennal mechanosensory neurons and further processed by second-order aPN1/aLN(al) neurons. However, little is known about the central pathways mediating courtship hearing. In this study, we identified a male-specific pathway for courtship hearing via third-order ventrolateral protocerebrum Projection Neuron 1 (vPN1) neurons and fourth-order pC1 neurons. Genetic inactivation of vPN1 or pC1 disrupts song-induced male-chaining behavior. Calcium imaging reveals that vPN1 responds preferentially to pulse song with long inter-pulse intervals (IPIs), while pC1 responses to pulse song closely match the behavioral chaining responses at different IPIs. Moreover, genetic activation of either vPN1 or pC1 induced courtship chaining, mimicking the behavioral response to song. These results outline the aPN1-vPN1-pC1 pathway as a labeled line for the processing and transformation of courtship song in males.