scholarly journals Central neural circuitry mediating courtship song perception in male Drosophila

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Chuan Zhou ◽  
Romain Franconville ◽  
Alexander G Vaughan ◽  
Carmen C Robinett ◽  
Vivek Jayaraman ◽  
...  
Keyword(s):  
Calcium Imaging ◽  
Behavioral Response ◽  
Projection Neuron ◽  
Neural Circuitry ◽  
Courtship Song ◽  
Third Order ◽  
Genetic Inactivation ◽  
Song Perception ◽  
Male Specific ◽  
Specific Pathway

Animals use acoustic signals across a variety of social behaviors, particularly courtship. In Drosophila, song is detected by antennal mechanosensory neurons and further processed by second-order aPN1/aLN(al) neurons. However, little is known about the central pathways mediating courtship hearing. In this study, we identified a male-specific pathway for courtship hearing via third-order ventrolateral protocerebrum Projection Neuron 1 (vPN1) neurons and fourth-order pC1 neurons. Genetic inactivation of vPN1 or pC1 disrupts song-induced male-chaining behavior. Calcium imaging reveals that vPN1 responds preferentially to pulse song with long inter-pulse intervals (IPIs), while pC1 responses to pulse song closely match the behavioral chaining responses at different IPIs. Moreover, genetic activation of either vPN1 or pC1 induced courtship chaining, mimicking the behavioral response to song. These results outline the aPN1-vPN1-pC1 pathway as a labeled line for the processing and transformation of courtship song in males.

scholarly journals Author response: Central neural circuitry mediating courtship song perception in male Drosophila

2015 ◽  
Author(s):  
Chuan Zhou ◽  
Romain Franconville ◽  
Alexander G Vaughan ◽  
Carmen C Robinett ◽  
Vivek Jayaraman ◽  
...  
Keyword(s):  
Neural Circuitry ◽  
Courtship Song ◽  
Author Response ◽  
Song Perception

scholarly journals Neural Changes Underlying Rapid Fly Song Evolution

2017 ◽  
Author(s):  
Yun Ding ◽  
Joshua L. Lillvis ◽  
Jessica Cande ◽  
Gordon J. Berman ◽  
Benjamin J. Arthur ◽  
...  
Keyword(s):  
Nervous System ◽  
Drosophila Melanogaster ◽  
Experimental Approach ◽  
Neural Circuits ◽  
Neural Circuitry ◽  
Courtship Song ◽  
Neural Basis ◽  
Electrophysiological Properties ◽  
Song Types ◽  
Behavioural Diversity

AbstractThe neural basis for behavioural evolution is poorly understood. Functional comparisons of homologous neurons may reveal how neural circuitry contributes to behavioural evolution, but homologous neurons cannot be identified and manipulated in most taxa. Here, we compare the function of homologous courtship song neurons by exporting neurogenetic reagents that label identified neurons in Drosophila melanogaster to D. yakuba. We found a conserved role for a cluster of brain neurons that establish a persistent courtship state. In contrast, a descending neuron with conserved electrophysiological properties drives different song types in each species. Our results suggest that song evolved, in part, due to changes in the neural circuitry downstream of this descending neuron. This experimental approach can be generalized to other neural circuits and therefore provides an experimental framework for studying how the nervous system has evolved to generate behavioural diversity.

scholarly journals Distinct neuropeptide-receptor modules regulate a sex-specific behavioral response to a pheromone

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Douglas K. Reilly ◽  
Emily J. McGlame ◽  
Elke Vandewyer ◽  
Annalise N. Robidoux ◽  
Caroline S. Muirhead ◽  
...  
Keyword(s):  
Motor Neurons ◽  
Behavioral Response ◽  
Receptor Expression ◽  
Specific Response ◽  
Sensory Cues ◽  
Animal Kingdom ◽  
Dioecious Species ◽  
C Elegans ◽  
Biochemical Studies ◽  
Male Specific

AbstractDioecious species are a hallmark of the animal kingdom, with opposing sexes responding differently to identical sensory cues. Here, we study the response of C. elegans to the small-molecule pheromone, ascr#8, which elicits opposing behavioral valences in each sex. We identify a novel neuropeptide-neuropeptide receptor (NP/NPR) module that is active in males, but not in hermaphrodites. Using a novel paradigm of neuropeptide rescue that we established, we leverage bacterial expression of individual peptides to rescue the sex-specific response to ascr#8. Concurrent biochemical studies confirmed individual FLP-3 peptides differentially activate two divergent receptors, NPR-10 and FRPR-16. Interestingly, the two of the peptides that rescued behavior in our feeding paradigm are related through a conserved threonine, suggesting that a specific NP/NPR combination sets a male state, driving the correct behavioral valence of the ascr#8 response. Receptor expression within pre-motor neurons reveals novel coordination of male-specific and core locomotory circuitries.

scholarly journals The Neural Circuitry Mediating Shifts in Behavioral Response and Cognitive Set in Autism

2008 ◽  
Vol 63 (10) ◽  
pp. 974-980 ◽  
Author(s):  
Keith M. Shafritz ◽  
Gabriel S. Dichter ◽  
Grace T. Baranek ◽  
Aysenil Belger
Keyword(s):  
Behavioral Response ◽  
Neural Circuitry ◽  
Cognitive Set

scholarly journals Decoding thalamic afferent input using microcircuit spiking activity

2015 ◽  
Vol 113 (7) ◽  
pp. 2921-2933 ◽  
Author(s):  
Audrey J. Sederberg ◽  
Stephanie E. Palmer ◽  
Jason N. MacLean
Keyword(s):  
Calcium Imaging ◽  
Activity Pattern ◽  
Behavioral Response ◽  
Relevant Information ◽  
Afferent Input ◽  
Small Population ◽  
Two Photon ◽  
Collective Activity ◽  
Cortical Connections ◽  
Cortical Microcircuit

A behavioral response appropriate to a sensory stimulus depends on the collective activity of thousands of interconnected neurons. The majority of cortical connections arise from neighboring neurons, and thus understanding the cortical code requires characterizing information representation at the scale of the cortical microcircuit. Using two-photon calcium imaging, we densely sampled the thalamically evoked response of hundreds of neurons spanning multiple layers and columns in thalamocortical slices of mouse somatosensory cortex. We then used a biologically plausible decoder to characterize the representation of two distinct thalamic inputs, at the level of the microcircuit, to reveal those aspects of the activity pattern that are likely relevant to downstream neurons. Our data suggest a sparse code, distributed across lamina, in which a small population of cells carries stimulus-relevant information. Furthermore, we find that, within this subset of neurons, decoder performance improves when noise correlations are taken into account.

scholarly journals Optogenetic Stimulation of Lateral Orbitofronto-Striatal Pathway Suppresses Compulsive Behaviors

Science ◽  
2013 ◽  
Vol 340 (6137) ◽  
pp. 1243-1246 ◽  
Author(s):  
Eric Burguière ◽  
Patrícia Monteiro ◽  
Guoping Feng ◽  
Ann M. Graybiel
Keyword(s):  
Response Inhibition ◽  
Behavioral Response ◽  
Repetitive Behavior ◽  
Projection Neuron ◽  
Repetitive Behaviors ◽  
Neuron Activity ◽  
Down Regulation ◽  
Delay Conditioning ◽  
Optogenetic Stimulation ◽  
Stimulation Of

Dysfunctions in frontostriatal brain circuits have been implicated in neuropsychiatric disorders, including those characterized by the presence of repetitive behaviors. We developed an optogenetic approach to block repetitive, compulsive behavior in a mouse model in which deletion of the synaptic scaffolding gene, Sapap3, results in excessive grooming. With a delay-conditioning task, we identified in the mutants a selective deficit in behavioral response inhibition and found this to be associated with defective down-regulation of striatal projection neuron activity. Focused optogenetic stimulation of the lateral orbitofrontal cortex and its terminals in the striatum restored the behavioral response inhibition, restored the defective down-regulation, and compensated for impaired fast-spiking neuron striatal microcircuits. These findings raise promising potential for the design of targeted therapy for disorders involving excessive repetitive behavior.

scholarly journals Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry

2020 ◽  
Author(s):  
Che-Hang Yu ◽  
Jeffrey N. Stirman ◽  
Yiyi Yu ◽  
Riichiro Hira ◽  
Spencer L. Smith
Keyword(s):  
Adaptive Optics ◽  
Calcium Imaging ◽  
High Speed ◽  
Optical Design ◽  
Neural Circuitry ◽  
Brain Regions ◽  
Field Of View ◽  
Large Field ◽  
Two Photon ◽  
Subcellular Resolution

AbstractImaging the activity of neurons that are widely distributed across brain regions deep in scattering tissue at high speed remains challenging. Here, we introduce an open-source system with Dual Independent Enhanced Scan Engines for Large Field-of-view Two-Photon imaging (Diesel2p). Combining novel optical design, adaptive optics, and temporal multiplexing, the system offers subcellular resolution over a large field-of-view (∼ 25 mm2) with independent scan engines. We demonstrate the flexibility and various use cases of this system for calcium imaging of neurons in the living brain.

scholarly journals Distinct Neuropeptide-Receptor Modules Regulate a Sex-Specific Behavioral Response to a Pheromone

2020 ◽  
Author(s):  
Douglas K. Reilly ◽  
Emily J. McGlame ◽  
Elke Vandewyer ◽  
Annalise M. Robidoux ◽  
Haylea T. Northcott ◽  
...  
Keyword(s):  
Motor Neurons ◽  
Behavioral Response ◽  
Receptor Expression ◽  
Specific Response ◽  
Sensory Cues ◽  
Animal Kingdom ◽  
Dioecious Species ◽  
C Elegans ◽  
Biochemical Studies ◽  
Male Specific

AbstractDioecious species are a hallmark of the animal kingdom, with opposing sexes responding differently to identical sensory cues. Here, we study the response of C. elegans’ to the small-molecule pheromone, ascr#8, which elicits opposing behavioral valences in each sex. We identify a novel neuropeptide-neuropeptide receptor (NP/NPR) module that is active in males, but not in hermaphrodites. Using a novel paradigm of neuropeptide rescue that we established, we leverage bacterial expression of individual peptides to rescue the sex-specific response to ascr#8. Concurrent biochemical studies confirmed individual FLP-3 peptides differentially activate two divergent receptors, NPR-10 and FRPR-16. Interestingly, the two of the peptides that rescued behavior in our feeding paradigm are related through a conserved threonine, suggesting that a specific NP/NPR combination sets a male state, driving the correct behavioral valence of the ascr#8 response. Receptor expression within pre-motor neurons reveals novel coordination of male-specific and core locomotory circuitries.

hermaphrodite and doublesex function both dependently and independently to control various aspects of sexual differentiation in Drosophila

Development ◽  
1998 ◽  
Vol 125 (14) ◽  
pp. 2641-2651 ◽  
Author(s):  
H. Li ◽  
B.S. Baker
Keyword(s):  
Sexual Differentiation ◽  
Target Genes ◽  
Terminal Differentiation ◽  
Anal Plate ◽  
Downstream Target ◽  
Specific Differentiation ◽  
Female Specific ◽  
Male Specific ◽  
Specific Pathway

The hermaphrodite (her) gene is necessary for sexual differentiation in Drosophila. Our characterization of her's zygotic function suggests that one set of female-specific terminal differentiation genes, the yolk protein (yp) genes, is transcriptionally activated by two separate pathways. One is a female-specific pathway, which is positively regulated by the female-specific doublesex protein (DSXF). The other is a non-sex-specific pathway, that is positively regulated by HER. The HER pathway is prevented from functioning in males by the action of the male-specific doublesex protein (DSXM). The HER and DSX pathways also function independently to control downstream target genes in the precursor cells that give rise to the vaginal teeth and dorsal anal plate of females, and the lateral anal plates of males. However, a female-specific pathway that is dependent on both DSXF and HER controls the female-specific differentiation of the foreleg bristles and tergites 5 and 6, and the male-specific differentiation of these tissues does not require the suppression of HER's function by DSXM.

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