Impregnation of a Bone Marrow Allograft with an Antibiotic Prepared According to the Marburg System, an Original Method

The aim: To develop an original method of antibiotic impregnation of bone allograft prepared according to the Marburg bone bank system. Methods. The work was divided into three parts (as part of an experimental study). The first part of the study included the collection and preparation of bone allograft, which was carried out on the basis of the Hospital named after professor Makazhanov, where the femoral heads were harvested, removed during arthroplasty and heat-treated according to the Marburg system of the bone bank. The second stage of the study was to select the optimal antibacterial drug for impregnation, by determining their thermal stability. The determination of thermal stability was carried out at the Department of Microbiology of the Karaganda Medical University. The third stage of the study included conducting experimental work on the development of an impregnation method and evaluating the antibacterial effectiveness of impregnated bone allografts. Results. Heat-treated bone allograft, perforated by the original method using a developed perforation device (the latter received a patent), and then soaked in an antibiotic, showed suppression of the bacterial growth zone by more than 2.5 times (p=0.01), in comparison with other groups. Conclusion. The proposed bone allograft is biodegradable and is close to autologous bone in its physical and mechanical properties. The optimal method of impregnation is bone allograft perforation, developed by an original method using a special perforation device. Keywords: bone allograft, Marburg system, bone bank, antibiotics, osteomyelitis, impregnation, osteo-replacement material.

Living donor bone bank: Costa Rican experience

All relevant information was gathered to provide a brief overview of the establishment of a Bone Bank in Costa Rica. First, legal issues were taken into consideration; followed by the definition of the donor exclusion criteria according to international standards. Potential donors were defined as all patients undergoing hip or knee replacement surgeries. In order to select the right donors, blood samples from all potential donors were tested for transmittable diseases. Bone tissues were obtained in the operating room to be processed later, under strict standardized conditions. Once the tissues were processed, allografts were stored at -80C until the transplantat procedures were carried out.

Configuration and Management of a Femoral Heads Bone Bank in a Specialised Tertiary Orthopaedic Hospital in Bucharest

The purpose of the current paper is to describe the functioning protocol of a femoral heads bone bank in a specialised tertiary orthopaedic hospital in Bucharest and the results of its activity between January 2011 and December 2016. Since the initiation of the femoral head bone bank in 2011, we selected and tested 761 donors of femoral heads. Out of them, we implanted 435 grafts to a total of 242 recipient patients. We performed a thorough donor screening process, which resulted in a significant proportion of femoral head allografts discarded, mainly due to a positive hepatitis B or/and C viruses test or a positive result of the bacterial culture from the bone graft (in 3% of the grafts); the offending micro-organism was a Methicillin-Sensible Staphylococcus spp. Our bone bank may serve as a framework for developing similar structures by other hospitals in our country or elsewhere.