Histological evaluation of bone matrix from bone bank allograft used in maxillofacial reconstruction

Background: Mandibular reconstruction, after extensive resection of the mandible for the treatment of oral cancer, is a well-known procedure, however, relatively little is known about bone integration into the titanium implant after reconstruction with a temporary plastic implant. The main goal of this experimental study was to study the process of osseous integration into the titanium implant in an in vivo experiment following prior mandibular reconstruction with a temporary plastic implant. Materials and Methods: Four ewes initially underwent a partial one-sided resection of the mandible, with the formation of an approximately 3 × 1 cm defect. All of the subjects received reconstruction with an implantation of a plastic plate (3 cm). The plastic plate was removed and replaced by a titanium implant at 1, 3, 6, and 12 months, accordingly. Both plastic and titanium implants were made via 3D-printing technology and personalized modeling. A total of 6 months after titanium implantation, a histological evaluation of biointegration was performed. Results: All surgeries were uncomplicated. The integration of osseous tissue into the titanium implant was seen in all cases. Histologically, each case showed variable integration of dense fibrotic tissue with fibroblasts and non-mature bone tissue with a definitive layer of bone matrix with many osteoblasts on the periphery. The prior implantation of the plastic plate did not interfere with bone integration into the titanium implant. Conclusion: Preliminary results demonstrated that a temporary plastic implant for mandibular reconstruction does not interfere with the consequent osseous biointegration of a permanent titanium implant. This shows that temporary reconstruction is a safe solution when delayed mandibular reconstruction is required due to disease severity.

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Histological Comparison in Rats between Carbonate Apatite Fabricated from Gypsum and Sintered Hydroxyapatite on Bone Remodeling

BioMed Research International ◽

10.1155/2015/579541 ◽

2015 ◽

Vol 2015 ◽

pp. 1-7 ◽

Cited By ~ 17

Author(s):

Yasunori Ayukawa ◽

Yumiko Suzuki ◽

Kanji Tsuru ◽

Kiyoshi Koyano ◽

Kunio Ishikawa

Keyword(s):

Bone Remodeling ◽

Cellular Response ◽

Bone Matrix ◽

Fibrous Tissue ◽

Bone Defects ◽

Histological Evaluation ◽

Precipitation Reaction ◽

Carbonate Apatite ◽

Osteoclastic Resorption ◽

Low Crystalline

Carbonate apatite (CO3Ap), the form of apatite found in bone, has recently attracted attention. The purpose of the present study was to histologically evaluate the tissue/cellular response toward the low-crystalline CO3Ap fabricated using a dissolution-precipitation reaction with set gypsum as a precursor. When set gypsum was immersed in a 100°C 1 mol/L Na3PO4aqueous solution for 24 h, the set gypsum transformed into CO3Ap. Both CO3Ap and sintered hydroxyapatite (s-HAp), which was used as a control, were implanted into surgically created tibial bone defects of rats for histological evaluation. Two and 4 weeks after the implantation, histological sections were created and observed using light microscopy. The CO3Ap granules revealed both direct apposition of the bone matrix by osteoblasts and osteoclastic resorption. In contrast, the s-HAp granules maintained their contour even after 4 weeks following implantation which implied that there was a lack of replacement into the bone. The s-HAp granules were sometimes encapsulated with fibrous tissue, and macrophage polykaryon was occasionally observed directly apposed to the implanted granules. From the viewpoint of bone remodeling, the CO3Ap granules mimicked the bone matrix, suggesting that CO3Ap may be an appropriate bone substitute.

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Radiographic and histological evaluation of ectopic application of deproteinized bovine bone matrix

Annals of Maxillofacial Surgery ◽

10.4103/2231-0746.186150 ◽

2016 ◽

Vol 6 (1) ◽

pp. 9 ◽

Cited By ~ 4

Author(s):

CarlaCastiglia Gonzaga ◽

RodrigoCarlos da Silva ◽

VivianeRozeira Crivellaro ◽

AllanFernando Giovanini ◽

Rafaela Scariot ◽

...

Keyword(s):

Bone Matrix ◽

Histological Evaluation ◽

Bovine Bone ◽

Deproteinized Bovine Bone

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Novel methods for demonstrating osseointegration of hydroxylapatite-coated titanium hip prostheses

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100084478 ◽

1991 ◽

Vol 49 ◽

pp. 34-35 ◽

Cited By ~ 1

Author(s):

P. Frayssinet ◽

J. Hanker ◽

D. Hardy ◽

B. Giammara

Keyword(s):

Hip Prosthesis ◽

Ethanol Concentration ◽

Bone Matrix ◽

Electron Microscopic Examination ◽

Electron Microscopic ◽

Hip Prostheses ◽

Cellular Inclusions ◽

Microscopic Studies ◽

Diamond Saw ◽

Plasma Sprayed

Prostheses implanted in hard tissues cannot be processed for electron microscopic examination or microanalysis in the same way as those in other tissues. For these reasons, we have developed methods allowing light and electron microscopic studies as well as microanalysis of the interface between bone and a metal biomaterial coated by plasma-sprayed hydroxylapatite(HA) ceramic.An HA-coated titanium hip prosthesis (Corail, Landos, France), which had been implanted for two years, was removed after death (unrelated to the orthopaedic problem). After fixation it was dehydrated in solutions of increasing ethanol concentration prior to embedment in polymethylmethacrylate(PMMA). Transverse femur sections were obtained with a diamond saw and the sections then carefully ground to a thickness of 200 microns. Plastic-embedded sections were stained for calcium with a silver methenamine modification of the von Kossa method for calcium staining and coated by carbon. They have been examined by back-scatter SEM on an ISI-SS60 operated at 25 KV. EDAX has been done on cellular inclusions and extracellular bone matrix.

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Histological evaluation of cochlear hair cell damage from noise-induced hearing loss in chinchillas

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100159217 ◽

1990 ◽

Vol 48 (3) ◽

pp. 332-333

Author(s):

R.V. Harrison ◽

R.J. Mount ◽

P. White ◽

N. Fukushima

Keyword(s):

Hair Cell ◽

Evoked Potential ◽

Cell Damage ◽

Acoustic Trauma ◽

Histological Evaluation ◽

Cell Integrity ◽

Functional Changes ◽

Cochlear Hair Cell ◽

Left And Right ◽

Contralateral Ear

In studies which attempt to define the influence of various factors on recovery of hair cell integrity after acoustic trauma, an experimental and a control ear which initially have equal degrees of damage are required. With in a group of animals receiving an identical level of acoustic trauma there is more symmetry between the ears of each individual, in respect to function, than between animals. Figure 1 illustrates this, left and right cochlear evoked potential (CAP) audiograms are shown for two chinchillas receiving identical trauma. For this reason the contralateral ear is used as control.To compliment such functional evaluations we have devised a scoring system, based on the condition of hair cell stereocilia as revealed by scanning electron microscopy, which permits total stereociliar damage to be expressed numerically. This quantification permits correlation of the degree of structural pathology with functional changes. In this paper wereport experiments to verify the symmetry of stereociliar integrity between two ears, both for normal (non-exposed) animals and chinchillas in which each ear has received identical noise trauma.

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Clinical and histological evaluation of thermal injury thresholds in human teeth: a preliminary study

Journal of Oral Rehabilitation ◽

10.1046/j.1365-2842.1997.00566.x ◽

1997 ◽

Vol 24 (11) ◽

pp. 791-801 ◽

Cited By ~ 91

Author(s):

P. BALDISSARA ◽

S. CATAPANO ◽

R. SCOTTI

Keyword(s):

Thermal Injury ◽

Histological Evaluation ◽

Human Teeth ◽

Preliminary Study

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The Effect of Dietary Glutathione and Coenzyme Q10 on the Prevention and Treatment of Inflammatory Bowel Disease in Mice

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831.74.1.74 ◽

2004 ◽

Vol 74 (1) ◽

pp. 74-85 ◽

Cited By ~ 5

Author(s):

Liu ◽

Russell ◽

Smith ◽

Bronson ◽

Milbury ◽

...

Keyword(s):

Inflammatory Bowel Disease ◽

Bowel Disease ◽

Dextran Sulfate ◽

Coenzyme Q ◽

Histological Evaluation ◽

Therapeutic Effects ◽

Prevention Study ◽

Prevention And Treatment ◽

Inflammatory Bowel ◽

Pre Treatment

Because reactive oxygen species have been implicated as mediators of inflammatory bowel disease (IBD), we evaluated the potential preventive and therapeutic effects of two dietary antioxidants, glutathione (GSH) and coenzyme Q10 (CoQ10) on dextran sulfate sodium (DSS)-induced colitis in mice. Fifty female 8-wk old Swiss-Webster mice were randomly assigned to 4 groups for a pre-treatment 'prevention' study: (1) GSH (1% of diet); (2) CoQ10 (200 mg/kg/d); (3) DSS only (3% of drinking water); (4) control (no treatment). The mice in groups 1 and 2 were fed with GSH or CoQ10 for 21 wks, and the mice in groups 1, 2 and 3 were provided DSS from wk 7 for 4 cycles (1 cycle = 1 wk DSS followed by 2-wk water). Another 50 mice were randomly assigned to 4 groups for a 21-wk 'treatment' study where the mice in groups 1, 2, and 3 were administered DSS for 6 cycles (18 wks) to induce colitis. GSH and CoQ10 were added from wk 7 until the completion of the protocol. Loose stools and hemocult positivity were modestly but significantly reduced with GSH or CoQ10 at several periods during the intervention in both the prevention and treatment studies. In contrast, histological evaluation revealed increases in colonic dysplasia and ulceration with GSH or CoQ10. Thus, in this mouse model, GSH and CoQ10 appear to have a beneficial effect on acute signs of IBD, but may have an adverse impact on the chronic pathophysiology of the disease. Further studies using additional animal models are required to determine whether GSH or CoQ10 provide a favorable or unfavorable benefit:risk ratio in the prevention or treatment of IBD.

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An unusual case of spinal cord restricted mycobacteriosis in a European mink

Tierärztliche Praxis Ausgabe K Kleintiere / Heimtiere ◽

10.1055/s-0038-1623679 ◽

2013 ◽

Vol 41 (01) ◽

pp. 63-66

Author(s):

D. Schaudien ◽

C. Flieshardt ◽

I. Moser ◽

H. Hotzel ◽

A. Tipold ◽

...

Keyword(s):

Spinal Cord ◽

Unusual Case ◽

Histological Evaluation ◽

Mustela Lutreola ◽

Chain Reaction ◽

European Mink ◽

Pcr Product ◽

Granulomatous Lesions ◽

The Central Nervous System ◽

Polymerase Chain

SummaryGranulomatous myelitis due to infection with Mycobacterium avium was diagnosed in a 4-year-old male neutered European mink (Mustela lutreola). The causative agent was detected by an acid-fast stain and further characterized by polymerase chain reaction and DNA sequencing of the PCR product. A thorough histological evaluation of the remaining organs revealed no granulomatous lesions or detectable acid-fast organisms. Although minks are generally highly susceptible for mycobacteria, localised infections, especially of the central nervous system, are unusual and may represent an atypical chronic form of the disease.

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