Environmental transmission of Pseudogymnoascus destructans to hibernating little brown bats

Pathogens with persistent environmental stages can have devastating effects on wildlife communities. White-nose syndrome (WNS), caused by the fungus Pseudogymnoascus destructans, has caused widespread declines in bat populations of North America. In 2009, during the early stages of the WNS investigation and before molecular techniques had been developed to readily detect P. destructans in environmental samples, we initiated this study to assess whether P. destructans can persist in the hibernaculum environment in the absence of its conclusive bat host and cause infections in naive bats. We transferred little brown bats (Myotis lucifugus) from an unaffected winter colony in northwest Wisconsin to two P. destructans contaminated hibernacula in Vermont where native bats had been excluded. Infection with P. destructans was apparent on some bats within 8 weeks following the introduction of unexposed bats to these environments, and mortality from WNS was confirmed by histopathology at both sites 14 weeks following introduction. These results indicate that environmental exposure to P. destructans is sufficient to cause the infection and mortality associated with WNS in naive bats, which increases the probability of winter colony extirpation and complicates conservation efforts.

Hepatic lipid signatures of little brown bats (Myotis lucifugus) and big brown bats (Eptesicus fuscus) at early stages of white-nose syndrome

White-nose syndrome (WNS) is an emergent wildlife fungal disease of cave-dwelling, hibernating bats that has led to unprecedented mortalities throughout North America. A primary factor in WNS-associated bat mortality includes increased arousals from torpor and premature fat depletion during winter months. Details of species and sex-specific changes in lipid metabolism during WNS are poorly understood and may play an important role in the pathophysiology of the disease. Given the likely role of fat metabolism in WNS and the fact that the liver plays a crucial role in fatty acid distribution and lipid storage, we assessed hepatic lipid signatures of little brown bats (Myotis lucifugus) and big brown bats (Eptesicus fuscus) at an early stage of infection with the etiological agent, Pseudogymnoascus destructans (Pd). Differences in lipid profiles were detected at the species and sex level in the sham-inoculated treatment, most strikingly in higher hepatic triacylglyceride (TG) levels in E. fuscus females compared to males. Interestingly, several dominant TGs (storage lipids) decreased dramatically after Pd infection in both female M. lucifugus and E. fuscus. Increases in hepatic glycerophospholipid (structural lipid) levels were only observed in M. lucifugus, including two phosphatidylcholines (PC [32:1], PC [42:6]) and one phosphatidylglycerol (PG [34:1]). These results suggest that even at early stages of WNS, changes in hepatic lipid mobilization may occur and be species and sex specific. As pre-hibernation lipid reserves may aid in bat persistence and survival during WNS, these early perturbations to lipid metabolism could have important implications for management responses that aid in pre-hibernation fat storage.

Personality affects dynamics of an experimental pathogen in little brown bats

Host behaviour can affect host–pathogen dynamics and theory predicts that certain individuals disproportionately infect conspecifics during an epidemic. Consistent individual differences in behaviour, or personality, could influence this variation with the most exploratory or sociable individuals most likely to spread pathogens. We quantified exploration and sociability in little brown bats ( Myotis lucifugus ) and then experimentally manipulated exposure to a proxy pathogen (i.e. ultraviolet (UV) fluorescent powder) to test two related hypotheses: (i) more sociable and more exploratory individuals would be more likely to transmit infections to other individuals, and (ii) more sociable and more exploratory individuals uninfected with an invading pathogen would be more likely to acquire infections. We captured 10 groups of 16 bats at a time and held each group in an outdoor flight tent equipped with roosting-boxes. We used hole-board and Y-maze tests to quantify exploration and sociability of each bat and randomly selected one individual from each group for ‘infection’ with non-toxic, UV fluorescent powder. Each group of 10 bats was released into the flight tent for 24 h, which represented an experimental infection trial. After 24 h, we removed bats from the trial, photographed each individual under UV light and quantified infection intensity from digital photographs. As predicted, the exploratory behaviour of the experimentally infected individual was positively correlated with infection intensity in their group-mates, while more exploratory females had higher pathogen acquisition. Our results highlight the potential influence of host personality and sex on pathogen dynamics in wildlife populations.

Exceptional Longevity in Little Brown Bats Still Occurs, despite Presence of White-Nose Syndrome

White-nose syndrome is an introduced fungal disease that has reduced the size of hibernating populations of little brown bats Myotis lucifugus by 90% across much of eastern North America since 2007. Herein, we report the recapture of eight banded little brown bats, all males, with minimum ages of 18.6–25.6 y. The recaptures occurred during winter 2019–2020, at a hibernaculum in Michigan where white-nose syndrome likely has been present since 2013–2014, indicating that these old and apparently healthy males are in their seventh season of exposure to the disease. Hence, our data suggest that a long life in little brown bats and existence of white-nose syndrome are not necessarily incompatible.

Little Brown Bats Utilize Multiple Maternity Roosts Within Foraging Areas: Implications for Identifying Summer Habitat

Identifying habitat features that may influence the survival and fitness of threatened species is often constrained by a lack of information about the appropriate scale for habitat conservation efforts. Canada's Species at Risk Act lists little brown bats Myotis lucifugus as Endangered and there is a need to determine the scale for delineating important summer habitat features that should be protected. We used a 19-y dataset of banded little brown bats in a 15,000-km2 area of southern Yukon, Canada, to examine fidelity to roost sites and potential foraging areas. We captured and banded 4,349 bats during 208 live-trapping sessions at maternity roosts. Adult females used multiple roosts during the maternity period, separated by up to 6.1 km, within foraging areas, to which individuals exhibited fidelity. Our fidelity rates (≤ 60.5%) are the lowest, and roost-switching rates (≤ 35.5%) the greatest, reported for little brown bats. A small percentage (14.0–20.7%) of females banded as juveniles returned to their natal roosts or foraging areas as adults. We infrequently observed long-distance (25–200 km) switching to novel foraging areas (< 1% of banded bats). We established bat houses to mitigate the loss of a cabin roost; 46.3% of the bats banded at the cabin occupied these houses. The longest documented period of roost fidelity was 18 y, by a female banded as an adult. Roost fidelity by returning adult females declined annually by 3.8–5.3% due to natural mortality, roost switching, or dispersal. Having a choice of multiple maternity roosts within a foraging area may permit little brown bats to select optimal microclimatic conditions throughout the maternity season. Given that fidelity to foraging areas may be higher than to specific roost sites for little brown bats, identification of summer habitat based on foraging areas may be a more effective conservation strategy than relying solely on roost sites.