Salmonella organism transmission in hatching broiler egggs

The vertical transmission of Salmonella organism in hatching broiler eggs were investigated in selected states in South-Western Nigeria. Hatching eggs were obtained from fifteen major hatcheries. five (hatchery A, B, C, D and E from each State [Lagos (LA), Oyo (OY) and Ogun (OG)]. A total of 300 hatchable eggs of 20 eggs from each hatchery were collected. Individual egg was broken and separated into Shell, albumin and yolk. The samples were incubated into buffer peptone water and incubated at 37°C for 24 hours. Typical colonies of salmonella grown on Xylose lysine deoxycholate agar were seen as lining black center and lightly transparent zone of reddish colour which were further cultured on nutrient agar plates for confirmatory test. The biochemical characterization was carried out using rapid kit for the identification of members of Enterobacteria family. The study revealed that Salmonella pullorum was isolated from the albumin in hatchery (OG-A) and hatchery (LA-A), Salmonella arizorae was also isolated in the Yolk from the hatchery (OG-C), Salmonella gallinarum was found in the albumin in Ogun hatchery (OG-A), however, Salmonella paratyphi A was isolated in the albumin in Oyo hatchery B (OY-B). It is evident from the study that salmonella organism reside in various fractions of the egg like albumin, shell and yolk.

Akurasi Perhitungan Bakteri pada Daging Sapi Menggunakan Metode Hitung Cawan

Daging sapi merupakan salah satu bahan makanan yang mengandung protein tinggi dan termasuk media yang baik bagi pertembuhan mikroorganisme seperti bakteri. Salah satu metode yang dapat digunakan untuk menghitung jumlah bakteri pada daging sapi yaitu metode hitung cawan. Beberapa cara yang dapat dilakukan pada metode hitung cawan yaitu metode pour plate, spread plate, dan drop plate. Penggunaan cara hitung cawan yang berbeda seta larutan pengencer yang berbeda menyebabkan hasil perhitungan yang berbeda juga. Tujuan dari penelitian ini yaitu untuk mengetahui perbedaan cara preparasi sampel serta larutan pengencer yang digunakan terhadap hasil hitung cawan bakteri pada sampel daging sapi. Metode hitung cawan yang digunakan pada penelitian ini yaitu pour plate, spread plate, dan drop plate dengan 3 jenis pelarut yaitu akuades steril, garam fisiologis 0,85% dan larutan Buffer Peptone Water (BPW) yang ditambahkan 1,25% Na Sitrat.Berdasarkan hasil penelitiandiketahui bahwa cara preparasi sampel daging sapi yang lebih efisien yaitu dengan cara dihaluskan ditunjukkan dengan hasil hitung cawan yaitu 106 CFU/gram dan hasil hitung tidak bervariasi dibandingkan daging sapi yang dipotong (105 CFU/gram) dengan hasil hitung yang bervariasi. Hasil analisis data menunjukkan bahwa penggunaan cara preparasi sampel yang berbeda berpengaruh pada hasil perhitungan jumlah koloni disetiap metode hitung cawan. Larutan buffer peptone water dan garam fisiologis 0,85% merupakan larutan pengencer yang lebih baik dibandingkan dengan larutan akuades. Hal ini ditunjukkan dengan hasil hitung yang tidak bervariasi disetiap metode hitung cawan yang digunakan. Berdasarkan hasil hitung yang didapatkan, larutan buffer peptone water menghasilkan hasil perhitungan jumlah koloni terbesar.

Detection of Drug-resistant S. aureus from Poultry Samples Collected from Different Areas of Bangladesh

Staphylococcus aureus is gram-positive cocci that can cause foodborne illness which can be transmitted by chicken meat, both raw and undercooked; consumption of which may cause infection and/or toxicity in consumers. This current study was conducted for the detection of the prevalence of S. aureus in three types of poultry samples which included chicken meat, chicken eggs and droppings. Samples were aseptically collected from different rural and urban areas of 8 districts of Bangladesh in triplicate collection method which was conducted in the Centre for Excellence, Department of Microbiology, Primeasia University, Dhaka, Bangladesh to detect the Multi Drug-Resistant (MDR) S. aureus. Isolation was done by using 7.5% Sodium Chloride broth for enrichment and Mannitol salt agar after enriching samples in Buffer Peptone water. Antibiogram was done by using Kirby Bauer Method on Muller Hinton agar. The current study revealed 68% (17/25), 84% (21/25) and 52% (13/25) of S. aureus in chicken meat, eggs and dropping, respectively. Around 75.47% isolates (40/53) showed 100% similarity with S. aureus from the generated Dendrogram based on biochemical data. Cefixime (62.67%) showed the highest sensitivity against all of the isolates while Penicillin (86.62%) exhibited the highest resistance. Moreover, 59.62% of isolates were resistant to 5 or more drugs (Multidrug-Resistant). Poultry products such as meat and eggs were contaminated with drug-resistant S. aureus which can cause serious health effect to the consumer. The drug-resistant pathogen in droppings helps to indicate the spread of drug-resistant isolates in the environment. Therefore, proper sanitation measures should be taken to ensure the quality of the products during slaughtering, collecting eggs or disposing of poultry litter.