Conservation of the Gharial (Gavialis gangeticus) at the Madras Crocodile Bank Trust, Chennai, India

Gavialis gangeticus are in serious decline in the wild. In this short article we provide an overview of Gharial ecology and current conservation measures taking place at the Madras Crocodile Bank Trust in India.

Anthropogenic restocking of gharial individuals prevents genetic isolation of gharial population in Girwa River, India by geographic barriers imposed by a barrage

Gharial (Gavialis gangeticus) is a critically endangered fresh water crocodile endemic to the Indian subcontinent. The species has suffered > 95% decline in population and habitat size. A small population of gharials comprising of 50 breeding adults is resident in a 20 Km stretch of the River Girwa in Katerniaghat wildlife sanctuary, India. Gharials in this 20 Km stretch have been genetically isolated since 1976 by a barrage that functions as a barrier to gene flow. A captive rear and release program has been initiated since 1979 under Project Crocodile for restocking declining wild gharial populations. Thousands of gharial eggs were collected from gharial populations at Girwa and Chambal Rivers. Hatchlings from the collected eggs were captive reared at a common location in Kukrail Gharial Centre, India and released back to multiple gharial populations including the isolated population at Girwa. This restocking programme was not preceded by a genetic screening of captive animals or wild populations to identify genetic diversity and genetic structure of both captive and wild animals. In this study we investigate whether release of captive reared gharials into the resident population at Girwa River has prevented genetic isolation caused due to barriers imposed by the barrage. Using a combination of empirical analysis using microsatellite markers and a systematic review of data from previous workers on molecular characterization of gharial populations, we analysed genetic differentiation in gharial populations at Girwa and Chambal. We found similar genetic variability in gharial populations of Girwa and Chambal. There was low inter population genetic differentiation and evidences of genetic migration between the two populations. Our findings indicate that anthropogenic intervention via release of captive animals has compensated for the genetic isolation in Girwa population caused by the barrage.

Phylogenetic analysis of a new morphological dataset elucidates the evolutionary history of Crocodylia and resolves the long-standing gharial problem

First appearing in the latest Cretaceous, Crocodylia is a clade of semi-aquatic, predatory reptiles, defined by the last common ancestor of extant alligators, caimans, crocodiles, and gharials. Despite large strides in resolving crocodylian interrelationships over the last three decades, several outstanding problems persist in crocodylian systematics. Most notably, there has been persistent discordance between morphological and molecular datasets surrounding the affinities of the extant gharials, Gavialis gangeticus and Tomistoma schlegelii. Whereas molecular data consistently support a sister taxon relationship, in which they are more closely related to crocodylids than to alligatorids, morphological data indicate that Gavialis is the sister taxon to all other extant crocodylians. Here we present a new morphological dataset for Crocodylia based on a critical reappraisal of published crocodylian character data matrices and extensive firsthand observations of a global sample of crocodylians. This comprises the most taxonomically comprehensive crocodylian dataset to date (144 OTUs scored for 330 characters) and includes a new, illustrated character list with modifications to the construction and scoring of characters, and 46 novel characters. Under a maximum parsimony framework, our analyses robustly recover Gavialis as more closely related to Tomistoma than to other extant crocodylians for the first time based on morphology alone. This result is recovered regardless of the weighting strategy and treatment of quantitative characters. However, analyses using continuous characters and extended implied weighting (with high k-values) produced the most resolved, well-supported, and stratigraphically congruent topologies overall. Resolution of the gharial problem reveals that: (1) several gavialoids lack plesiomorphic features that formerly drew them towards the stem of Crocodylia; and (2) more widespread similarities occur between species traditionally divided into tomistomines and gavialoids, with these interpreted here as homology rather than homoplasy. There remains significant temporal incongruence regarding the inferred divergence timing of the extant gharials, indicating that several putative gavialids (‘thoracosaurs’) are incorrectly placed and require future re-appraisal. New alligatoroid interrelationships include: (1) support for a North American origin of Caimaninae in the latest Cretaceous; (2) the recovery of the early Paleogene South American taxon Eocaiman as a ‘basal’ alligatoroid; and (3) the paraphyly of the Cenozoic European taxon Diplocynodon. Among crocodyloids, notable results include modifications to the taxonomic content of Mekosuchinae, including biogeographic affinities of this clade with latest Cretaceous–early Paleogene Asian crocodyloids. In light of our new results, we provide a comprehensive review of the evolutionary and biogeographic history of Crocodylia, which included multiple instances of transoceanic and continental dispersal.

Microsatellite analysis reveals low genetic diversity in managed populations of the critically endangered gharial (Gavialis gangeticus) in India

The gharial (Gavialis gangeticus) is a critically endangered crocodylian, endemic to the Indian subcontinent. The species has experienced severe population decline during the twentieth century owing to habitat loss, poaching, and mortalities in passive fishing. Its extant populations have largely recovered through translocation programmes initiated in 1975. Understanding the genetic status of these populations is crucial for evaluating the effectiveness of the ongoing conservation efforts. This study assessed the genetic diversity, population structure, and evidence of genetic bottlenecks of the two managed populations inhabiting the Chambal and Girwa Rivers, which hold nearly 80% of the global gharial populations. We used seven polymorphic nuclear microsatellite loci and a 520 bp partial fragment of the mitochondrial control region (CR). The overall mean allelic richness (Ar) was 2.80 ± 0.40, and the observed (Ho) and expected (He) heterozygosities were 0.40 ± 0.05 and 0.39 ± 0.05, respectively. We observed low levels of genetic differentiation between populations (FST = 0.039, P < 0.05; G’ST = 0.058, P < 0.05 Jost’s D = 0.016, P < 0.05). The bottleneck analysis using the M ratio (Chambal = 0.31 ± 0.06; Girwa = 0.41 ± 0.12) suggested the presence of a genetic bottleneck in both populations. The mitochondrial CR also showed a low level of variation, with two haplotypes observed in the Girwa population. This study highlights the low level of genetic diversity in the two largest managed gharial populations in the wild. Hence, it is recommended to assess the genetic status of extant wild and captive gharial populations for planning future translocation programmes to ensure long-term survival in the wild.

Mitochondrial DNA analysis reveals extremely low genetic diversity in a managed population of the Critically Endangered Gharial (Gavialis gangeticus, Gmelin, 1789)

A decline in the numbers of threatened species is often reversed by reintroduction with the aim of repopulating or strengthening the population to reduce the risk of extinction. The success of reintroduction programs is associated with demographic and genetic monitoring of the reintroduced populations. We undertook a genetic assessment of the Critically Endangered gharial (Gavialis gangeticus) to assess the current level of genetic variation using three partial mitochondrial (mt) DNA regions: cytochrome b, cytochrome c oxidase subunit-I and the control region. We sequenced 103 samples collected across 14 nesting sites. A low level of mtDNA variation was observed in the sampled population (hd = 0.462 ± 0.048; Pi = 0.00029 ± 0.00004). Only five distinct haplotypes were observed in three segregating sites. This is the first assessment of the genetic variation in the wild gharial population to be made using mtDNA. Homogeneity in the 520 bp hypervariable control region of the crocodilian mtDNA is reported here for the first time. The low mitochondrial diversity and no genetic structure in the sampled population is indicative of a genetic bottleneck, the founder effect and probably associated with humanassisted augmentation of the population of the gharial. An extremely low level of genetic variation in the largest gharial population highlights the vulnerability of the gharial population in the wild and calls for immediate genetic assessment of other gharial populations so that a robust conservation plan focusing on connectivity and enhanced protection can be developed for the long-term persistence of the gharial in the wild.