In vitro Comparative Tests About the Biocompatibility of Some Dental Alloys

We evaluated the biocompatibility of four types of commercial alloys (two CoCr alloys and two NiCr alloys) used to make dental bridges. For the cell biology tests, a human osteosarcome type culture cell line MG63 (American Type Culture Collection) was used. Taking into account the results obtained, it can be said that the best results in terms of cell proliferation were observed for the Ni-Cr / Solibond N alloy closely followed by Co-Cr / Heraenium CE, then Co-Cr / Solibond C and Ni-Cr / Kera N, while cell viability tests revealed that the Co-Cr / Heraenium CE alloy exhibits the best biocompatibility, followed by Ni-Cr / Kera N, Co-Cr / Solibond C and Ni-Cr / Solibond N.

In Vitro Cytotoxicity of Bacterial Cellulose Scaffolds Used for Tissue-engineered Bone

The in vitro degradation and cytotoxicity of bacterial cellulose (BC) and its degradation products were studied for potential applications in bone tissue engineering. Emission scanning electron microscope was used to observe the morphology of original materials and their degradation products. The degradation was evaluated by measuring the concentration of reducing sugar by using ultraviolet spectrophotometer. Bone forming osteoblast (OB) cells and infinite culture cell line L929 fibroblasts were used to measure the cytotoxicity of materials using the MTT assay. Both types of cells proliferated normally with the BC and its degradation products with a cytotoxicity graded of 0–1. Nevertheless, the bone-forming target OB cells were more susceptible to cytotoxicity than the infinite culture fibroblast cells L929 fibroblasts. The results indicate that the BC is not very cytotoxic and that tissue functional cells are more suitable for evaluating the cytotoxicity of biomedical materials.