In Vitro Cytotoxicity of Bacterial Cellulose Scaffolds Used for Tissue-engineered Bone

2009 ◽  
Vol 24 (1_suppl) ◽  
pp. 137-145 ◽  
Author(s):  
Y.M. Chen ◽  
Tingfei Xi ◽  
Yudong Zheng ◽  
Tingting Guo ◽  
Jiaquan Hou ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Degradation Products ◽  
Culture Cell ◽  
In Vitro Cytotoxicity ◽  
Reducing Sugar ◽  
Fibroblast Cells ◽  
In Vitro Degradation ◽  
Culture Cell Line ◽  
Potential Applications

The in vitro degradation and cytotoxicity of bacterial cellulose (BC) and its degradation products were studied for potential applications in bone tissue engineering. Emission scanning electron microscope was used to observe the morphology of original materials and their degradation products. The degradation was evaluated by measuring the concentration of reducing sugar by using ultraviolet spectrophotometer. Bone forming osteoblast (OB) cells and infinite culture cell line L929 fibroblasts were used to measure the cytotoxicity of materials using the MTT assay. Both types of cells proliferated normally with the BC and its degradation products with a cytotoxicity graded of 0–1. Nevertheless, the bone-forming target OB cells were more susceptible to cytotoxicity than the infinite culture fibroblast cells L929 fibroblasts. The results indicate that the BC is not very cytotoxic and that tissue functional cells are more suitable for evaluating the cytotoxicity of biomedical materials.

Calcium hypochlorite on mouse embryonic fibroblast cells (NIH3T3) in vitro cytotoxicity and genotoxicity: MTT and comet assay

2020 ◽  
Vol 47 (7) ◽  
pp. 5377-5383
Author(s):  
Şehnaz Yilmaz ◽  
Oguz Yoldas ◽  
Aysin Dumani ◽  
Gizem Guler ◽  
Seda Ilgaz ◽  
...  
Keyword(s):  
Comet Assay ◽  
Mouse Embryonic Fibroblast ◽  
In Vitro Cytotoxicity ◽  
Fibroblast Cells ◽  
Calcium Hypochlorite ◽  
Embryonic Fibroblast

In Vitro Cytotoxicity of Injectable and Biodegradable Poly(propylene fumarate)-Based Networks:  Unreacted Macromers, Cross-Linked Networks, and Degradation Products

2003 ◽  
Vol 4 (4) ◽  
pp. 1026-1033 ◽  
Author(s):  
Mark D. Timmer ◽  
Heungsoo Shin ◽  
R. Adam Horch ◽  
Catherine G. Ambrose ◽  
Antonios G. Mikos
Keyword(s):  
Degradation Products ◽  
In Vitro Cytotoxicity ◽  
Biodegradable Poly ◽  
Poly Propylene

In Vitro Cytotoxicity Study of the Nano-Hydroxyapatite/Bacterial Cellulose Nanocomposites

2009 ◽  
Vol 610-613 ◽  
pp. 1011-1016 ◽  
Author(s):  
Yan Mei Chen ◽  
Ting Fei Xi ◽  
Yu Dong Zheng ◽  
Yi Zao Wan
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bacterial Cellulose ◽  
Bone Tissue ◽  
Biological Response ◽  
Biological Properties ◽  
In Vitro Cytotoxicity ◽  
National Standard ◽  
Target Cells

The nanocomposite of nano-hydroxyapatite/bacterial cellulose (nHA/BC) obtained by depositing in simulated body fluid (SBF), incorporating their excellent mechanical and biological properties, is expected to have potential applications in bone tissue engineering. However, the biological response evaluation of biomaterials is required to provide useful information to improve their design and application. In this article, the in vitro cytotoxicity of composites nHA/BC as well as its degradation residues was studied. Scanning electron microscopy (SEM) was used to observe the morphology of original materials and their degradation residues. The degree of degradation was evalued by measuring the concentration of reducing sugar (glucose) by ultraviolet spectrophotometer. Bone-forming osteoblasts (OB) and infinite culture cell line L929 fibroblasts were used to measure the cytotocixity of materials with MTT assay. Both kinds of cells in infusion proliferate greatly in a normal form and their relative growth rate (RGR) exceeds by 75%, which shows the cytotoxicity of materials is graded as 0~1, according to the national standard. Nevertheless, bone-forming OB cells, as a kind of target cells, are more susceptive on the cytotoxicity than infinite culture fibroblast cells L929. The results suggest the nanocomposite of nHA/BC without cytotoxicity is greatly promising as a kind of scaffold materials for bone tissue engineering and tissue functional cells are more suited to evaluate the cytotoxicity of biomedical materials.

scholarly journals Preparation and evaluation of chitosan based thermoreversible gels for intraperitoneal delivery of 5-fluorouracil (5-FU)

2013 ◽  
Vol 63 (4) ◽  
pp. 479-491 ◽  
Author(s):  
Bhavesh P. Depani ◽  
Anuja A. Naik ◽  
Hema A. Nair
Keyword(s):  
Antineoplastic Agent ◽  
In Vitro Cytotoxicity ◽  
In Vivo Studies ◽  
Comparable Efficacy ◽  
Prolonged Release ◽  
Fibroblast Cells ◽  
Glycerol Phosphate ◽  
Drug Free

Abstract Sterile thermoreversibly gelling systems based on chitosan- glycerol phosphate were developed for intraperitoneal delivery of the antineoplastic agent 5-FU. The formulation was evaluated for gelling characteristics and in vitro drug release. Drug free gels were evaluated for in vitro cytotoxicity in L-929 mouse fibroblast cells. Drug loaded gels were subjected to acute toxicity studies in Swiss albino mice via intraperitoneal route and efficacy studies via intratumoral injections in subcutaneous colon carcinoma bearing BALB/c mice. The formulations gelled reversibly in 8 min at 37 °C and provided prolonged release of the drug. Drug free systems showed dose dependent cytotoxicity in fibroblast cells, while in vivo studies revealed a 2.8-fold increase in LD50 of 5-FU administered intraperitoneally as the developed system. Tumor volume measurements showed comparable efficacy of 5-FU administered as gel and commercial injection with a greatly improved safety profile of the former as adjudged from mortality and body weight measurements.

scholarly journals Identification, isolation, structural characterization, in silico toxicity prediction and in vitro cytotoxicity assay of simeprevir acidic and oxidative degradation products

RSC Advances ◽  
2020 ◽  
Vol 10 (70) ◽  
pp. 42816-42826
Author(s):  
Rasha M. Ahmed ◽  
Marwa A. A. Fayed ◽  
Mohammed F. El-Behairy ◽  
Inas A. Abdallah
Keyword(s):  
Chronic Hepatitis ◽  
Degradation Products ◽  
Antiviral Drug ◽  
Oxidative Degradation ◽  
In Vitro Cytotoxicity ◽  
Toxicity Prediction ◽  
Direct Acting Antiviral ◽  
In Silico Toxicity ◽  
Direct Acting

Simeprevir is a new direct-acting antiviral drug used for the treatment of chronic hepatitis C.

Preparation, Cytotoxicity and Degradability of Chitosan-Based Thermosensitive Hydrogels as Drug Delivery System

2009 ◽  
Vol 79-82 ◽  
pp. 1531-1534 ◽  
Author(s):  
Hui Yun Zhou ◽  
Jian Wang ◽  
Wei Fen Zhang ◽  
Ye Wang ◽  
Xi Guang Chen
Keyword(s):  
In Vitro Cytotoxicity ◽  
Embryonic Fibroblasts ◽  
Thermosensitive Hydrogel ◽  
In Vitro Degradation ◽  
Relative Growth ◽  
Mtt Method ◽  
Accepted Standard ◽  
Thermosensitive Hydrogels ◽  
Model Drug

A new thermosensitive hydrogel had been prepared that could be transformed into gel at 37 °C from chitosan and a mixture of α- and β-glycerophosphate (αβ-GP). The appearance of hydrogel was compact and corrugated. There was little granule in the appearance of gel loaded with adriamycin and the granules might be crystals of the added model drug. In vitro cytotoxicity of the hydrogel was tested by the MTT method using mouse embryonic fibroblasts (MEF). MEF cultured with leachates of CS-αβ-GP were investigated and the relative growth rate (RGR) was calculated and the cytotoxicity was graded by generally accepted standard. The study of in vitro degradation of CS-αβ-GP hydrogels included hydrolysis and degradation by lysozyme. The CS-αβ-GP thermosensitive hydrogel was degradable in vitro and the degradation rate was faster in lysozyme solution than that in the medium of PBS. So the CS-αβ-GP system had good cell biocompatibility and biodegradability which provided possibilities and foundations for the further research.

scholarly journals In Vitro Degradation of Poly(caprolactone)/nHA Composites

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Esperanza Díaz ◽  
Iban Sandonis ◽  
María Blanca Valle
Keyword(s):  
Mechanical Properties ◽  
Degradation Products ◽  
Degradation Process ◽  
Porous Scaffolds ◽  
Degradation Behavior ◽  
Compressive Properties ◽  
Composite Scaffolds ◽  
In Vitro Degradation ◽  
Poly Caprolactone

The degradation behavior and mechanical properties of polycaprolactone/nanohydroxyapatite composite scaffolds are studied in phosphate buffered solution (PBS), at 37°C, over 16 weeks. Under scanning electron microscopy (SEM), it was observed that the longer the porous scaffolds remained in the PBS, the more significant the thickening of the pore walls of the scaffold morphology was. A decrease in the compressive properties, such as the modulus and the strength of the PCL/nHA composite scaffolds, was observed as the degradation experiment progressed. Samples with high nHA concentrations degraded more significantly in comparison to those with a lower content. Pure PCL retained its mechanical properties comparatively well in the study over the period of degradation. After the twelfth week, the results obtained by GPC analysis indicated a significant reduction in their molecular weight. The addition of nHA particles to the scaffolds accelerated the weight loss of the composites and increased their capacity to absorb water during the initial degradation process. The addition of these particles also affected the degradation behavior of the composite scaffolds, although they were not effective at compensating the decrease in pH prompted by the degradation products of the PCL.

Sign in / Sign up

Export Citation Format

Share Document