Artificial polyhydroxyalkanoate poly[2-hydroxybutyrate-block-3-hydroxybutyrate] elastomer-like material

The first polyhydroxyalkanoate (PHA) block copolymer poly(2-hydroxybutyrate-b-3-hydroxybutyrate) [P(2HB-b-3HB)] was previously synthesized using engineered Escherichia coli expressing a chimeric PHA synthase PhaCAR with monomer sequence-regulating capacity. In the present study, the physical properties of the block copolymer and its relevant random copolymer P(2HB-ran-3HB) were evaluated. Stress–strain tests on the P(88 mol% 2HB-b-3HB) film showed an increasing stress value during elongation up to 393%. In addition, the block copolymer film exhibited slow contraction behavior after elongation, indicating that P(2HB-b-3HB) is an elastomer-like material. In contrast, the P(92 mol% 2HB-ran-3HB) film, which was stretched up to 692% with nearly constant stress, was stretchable but not elastic. The differential scanning calorimetry and wide-angle X-ray diffraction analyses indicated that the P(2HB-b-3HB) contained the amorphous P(2HB) phase and the crystalline P(3HB) phase, whereas P(2HB-ran-3HB) was wholly amorphous. Therefore, the elasticity of P(2HB-b-3HB) can be attributed to the presence of the crystalline P(3HB) phase and a noncovalent crosslinked structure by the crystals. These results show the potential of block PHAs as elastic materials.

Perceptual judgments for the softness of materials under indentation

Humans can judge the softness of elastic materials through only visual cues. However, factors contributing to the judgement of visual softness are not yet fully understood. We conducted a psychophysical experiment to determine which factors and motion features contribute to the apparent softness of materials. Observers watched video clips in which materials were indented from the top surface to a certain depth, and reported the apparent softness of the materials. The depth and speed of indentation were systematically manipulated. As physical characteristics of materials, compliance was also controlled. It was found that higher indentation speeds resulted in larger softness rating scores and the variation with the indentation speed was successfully explained by the image motion speed. The indentation depth had a powerful effect on the softness rating scores whose variation with the indentation depth was consistently explained by motion features related to overall deformation. Higher material compliance resulted in higher rating scores while their effect was not straightforwardly explained by the motion features. We conclude that the brain makes visual judgments about the softness of materials under indentation on the basis of the motion speed and deformation magnitude while motion features related to material compliance require further study.